Abstract
An immunochromatographic test system was developed for rapid detection of the levels of specific IgG antibodies to Brucella abortus lipopolysaccharide, as a tool for diagnosis of brucellosis in cattle. The pilot test strips were examined using blood sera from sick (78 samples) and healthy (35 samples) cows. The results obtained by immunochromatographic assay, using a portable optical densitometer for digital video detection, correlate well with the results obtained by immunoenzyme assay and are in agreement with the results of the disease diagnosis. The new test system allows detection of antibodies within 10 min and can be proposed as an alternative to the methods available for serodiagnosis of brucellosis.
| Original language | English |
|---|---|
| Pages (from-to) | 80-90 |
| Number of pages | 11 |
| Journal | Journal of Immunoassay and Immunochemistry |
| Volume | 36 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2 2015 |
| Externally published | Yes |
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Keywords
- brucellosis
- diagnostics
- immunochromatographic assay
- rapid test
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Clinical Biochemistry
- Medical Laboratory Technology
- Medicine(all)
Cite this
Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level. / Sotnikov, Dmitriy V.; Byzova, Nadezhda A.; Zherdev, Anatoly V.; Eskendirova, Saule Z.; Baltin, Kairat K.; Mukanov, Kasim K.; Ramankulov, Erlan M.; Sadykhov, Elchin G.; Dzantiev, Boris B.
In: Journal of Immunoassay and Immunochemistry, Vol. 36, No. 1, 02.01.2015, p. 80-90.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level
AU - Sotnikov, Dmitriy V.
AU - Byzova, Nadezhda A.
AU - Zherdev, Anatoly V.
AU - Eskendirova, Saule Z.
AU - Baltin, Kairat K.
AU - Mukanov, Kasim K.
AU - Ramankulov, Erlan M.
AU - Sadykhov, Elchin G.
AU - Dzantiev, Boris B.
PY - 2015/1/2
Y1 - 2015/1/2
N2 - An immunochromatographic test system was developed for rapid detection of the levels of specific IgG antibodies to Brucella abortus lipopolysaccharide, as a tool for diagnosis of brucellosis in cattle. The pilot test strips were examined using blood sera from sick (78 samples) and healthy (35 samples) cows. The results obtained by immunochromatographic assay, using a portable optical densitometer for digital video detection, correlate well with the results obtained by immunoenzyme assay and are in agreement with the results of the disease diagnosis. The new test system allows detection of antibodies within 10 min and can be proposed as an alternative to the methods available for serodiagnosis of brucellosis.
AB - An immunochromatographic test system was developed for rapid detection of the levels of specific IgG antibodies to Brucella abortus lipopolysaccharide, as a tool for diagnosis of brucellosis in cattle. The pilot test strips were examined using blood sera from sick (78 samples) and healthy (35 samples) cows. The results obtained by immunochromatographic assay, using a portable optical densitometer for digital video detection, correlate well with the results obtained by immunoenzyme assay and are in agreement with the results of the disease diagnosis. The new test system allows detection of antibodies within 10 min and can be proposed as an alternative to the methods available for serodiagnosis of brucellosis.
KW - brucellosis
KW - diagnostics
KW - immunochromatographic assay
KW - rapid test
UR - http://www.scopus.com/inward/record.url?scp=84906691048&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84906691048&partnerID=8YFLogxK
U2 - 10.1080/15321819.2014.896266
DO - 10.1080/15321819.2014.896266
M3 - Article
VL - 36
SP - 80
EP - 90
JO - Journal of Immunoassay and Immunochemistry
JF - Journal of Immunoassay and Immunochemistry
SN - 1532-1819
IS - 1
ER -