Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level

Dmitriy V. Sotnikov, Nadezhda A. Byzova, Anatoly V. Zherdev, Saule Z. Eskendirova, Kairat K. Baltin, Kasim K. Mukanov, Erlan M. Ramankulov, Elchin G. Sadykhov, Boris B. Dzantiev

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An immunochromatographic test system was developed for rapid detection of the levels of specific IgG antibodies to Brucella abortus lipopolysaccharide, as a tool for diagnosis of brucellosis in cattle. The pilot test strips were examined using blood sera from sick (78 samples) and healthy (35 samples) cows. The results obtained by immunochromatographic assay, using a portable optical densitometer for digital video detection, correlate well with the results obtained by immunoenzyme assay and are in agreement with the results of the disease diagnosis. The new test system allows detection of antibodies within 10 min and can be proposed as an alternative to the methods available for serodiagnosis of brucellosis.

Original languageEnglish
Pages (from-to)80-90
Number of pages11
JournalJournal of Immunoassay and Immunochemistry
Volume36
Issue number1
DOIs
Publication statusPublished - Jan 2 2015
Externally publishedYes

Fingerprint

Brucella abortus
Brucellosis
Assays
Immunochromatography
Densitometers
Antibodies
Serologic Tests
Serum
Lipopolysaccharides
Blood
Immunoglobulin G

Keywords

  • brucellosis
  • diagnostics
  • immunochromatographic assay
  • rapid test

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Medicine(all)

Cite this

Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level. / Sotnikov, Dmitriy V.; Byzova, Nadezhda A.; Zherdev, Anatoly V.; Eskendirova, Saule Z.; Baltin, Kairat K.; Mukanov, Kasim K.; Ramankulov, Erlan M.; Sadykhov, Elchin G.; Dzantiev, Boris B.

In: Journal of Immunoassay and Immunochemistry, Vol. 36, No. 1, 02.01.2015, p. 80-90.

Research output: Contribution to journalArticle

Sotnikov, Dmitriy V. ; Byzova, Nadezhda A. ; Zherdev, Anatoly V. ; Eskendirova, Saule Z. ; Baltin, Kairat K. ; Mukanov, Kasim K. ; Ramankulov, Erlan M. ; Sadykhov, Elchin G. ; Dzantiev, Boris B. / Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level. In: Journal of Immunoassay and Immunochemistry. 2015 ; Vol. 36, No. 1. pp. 80-90.
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