Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level

Dmitriy V. Sotnikov; Nadezhda A. Byzova; Anatoly V. Zherdev; Saule Z. Eskendirova; Kairat K. Baltin; Kasim K. Mukanov; Erlan M. Ramankulov; Elchin G. Sadykhov; Boris B. Dzantiev

doi:10.1080/15321819.2014.896266

Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level

Dmitriy V. Sotnikov, Nadezhda A. Byzova, Anatoly V. Zherdev, Saule Z. Eskendirova, Kairat K. Baltin, Kasim K. Mukanov, Erlan M. Ramankulov, Elchin G. Sadykhov, Boris B. Dzantiev

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

An immunochromatographic test system was developed for rapid detection of the levels of specific IgG antibodies to Brucella abortus lipopolysaccharide, as a tool for diagnosis of brucellosis in cattle. The pilot test strips were examined using blood sera from sick (78 samples) and healthy (35 samples) cows. The results obtained by immunochromatographic assay, using a portable optical densitometer for digital video detection, correlate well with the results obtained by immunoenzyme assay and are in agreement with the results of the disease diagnosis. The new test system allows detection of antibodies within 10 min and can be proposed as an alternative to the methods available for serodiagnosis of brucellosis.

Original language	English
Pages (from-to)	80-90
Number of pages	11
Journal	Journal of Immunoassay and Immunochemistry
Volume	36
Issue number	1
DOIs	https://doi.org/10.1080/15321819.2014.896266
Publication status	Published - Jan 2 2015
Externally published	Yes

Keywords

brucellosis
diagnostics
immunochromatographic assay
rapid test

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Medical Laboratory Technology

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Sotnikov, D. V., Byzova, N. A., Zherdev, A. V., Eskendirova, S. Z., Baltin, K. K., Mukanov, K. K., Ramankulov, E. M., Sadykhov, E. G., & Dzantiev, B. B. (2015). Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level. Journal of Immunoassay and Immunochemistry, 36(1), 80-90. https://doi.org/10.1080/15321819.2014.896266

Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level. / Sotnikov, Dmitriy V.; Byzova, Nadezhda A.; Zherdev, Anatoly V.; Eskendirova, Saule Z.; Baltin, Kairat K.; Mukanov, Kasim K.; Ramankulov, Erlan M.; Sadykhov, Elchin G.; Dzantiev, Boris B.

In: Journal of Immunoassay and Immunochemistry, Vol. 36, No. 1, 02.01.2015, p. 80-90.

Research output: Contribution to journal › Article › peer-review

Sotnikov, DV, Byzova, NA, Zherdev, AV, Eskendirova, SZ, Baltin, KK, Mukanov, KK, Ramankulov, EM, Sadykhov, EG & Dzantiev, BB 2015, 'Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level', Journal of Immunoassay and Immunochemistry, vol. 36, no. 1, pp. 80-90. https://doi.org/10.1080/15321819.2014.896266

Sotnikov, Dmitriy V. ; Byzova, Nadezhda A. ; Zherdev, Anatoly V. ; Eskendirova, Saule Z. ; Baltin, Kairat K. ; Mukanov, Kasim K. ; Ramankulov, Erlan M. ; Sadykhov, Elchin G. ; Dzantiev, Boris B. / Express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level. In: Journal of Immunoassay and Immunochemistry. 2015 ; Vol. 36, No. 1. pp. 80-90.

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