Expression of auxin-binding protein1 during plum fruit ontogeny supports the potential role of auxin in initiating and enhancing climacteric ripening

I. El-Sharkawy, S. Sherif, A. Mahboob, K. Abubaker, M. Bouzayen, S. Jayasankar

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Auxin-binding protein1 (ABP1) is an active element involved in auxin signaling and plays critical roles in auxin-mediated plant development. Here, we report the isolation and characterization of a putative sequence from Prunus salicina L., designated PslABP1. The expected protein exhibits a similar molecular structure to that of well-characterized maize-ABP1; however, PslABP1 displays more sequence polarity in the active-binding site due to substitution of some crucial amino-acid residues predicted to be involved in auxin-binding. Further, PslABP1 expression was assessed throughout fruit ontogeny to determine its role in fruit development. Comparing the expression data with the physiological aspects that characterize fruit-development stages indicates that PslABP1 up-regulation is usually associated with the signature events that are triggered in an auxin-dependent manner such as floral induction, fruit initiation, embryogenesis, and cell division and elongation. However, the diversity in PslABP1 expression profile during the ripening process of early and late plum cultivars seems to be due to the variability of endogenous auxin levels among the two cultivars, which consequently can change the levels of autocatalytic ethylene available for the fruit to co-ordinate ripening. The effect of auxin on stimulating ethylene production and in regulating PslABP1 was investigated. Our data suggest that auxin is involved in the transition of the mature green fruit into the ripening phase and in enhancing the ripening process in both auxin- and ethylene-dependent manners thereafter.

Original languageEnglish
Pages (from-to)1911-1921
Number of pages11
JournalPlant Cell Reports
Volume31
Issue number10
DOIs
Publication statusPublished - Oct 2012
Externally publishedYes

Fingerprint

plums
ontogeny
auxins
ripening
fruits
fruiting
ethylene
Prunus salicina
cultivars
ethylene production
chemical structure
plant development
cell division
binding sites
cell growth
embryogenesis
amino acids
corn

Keywords

  • Auxin
  • Fruit development and ripening
  • Protein structure
  • Subcellular localization

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Expression of auxin-binding protein1 during plum fruit ontogeny supports the potential role of auxin in initiating and enhancing climacteric ripening. / El-Sharkawy, I.; Sherif, S.; Mahboob, A.; Abubaker, K.; Bouzayen, M.; Jayasankar, S.

In: Plant Cell Reports, Vol. 31, No. 10, 10.2012, p. 1911-1921.

Research output: Contribution to journalArticle

El-Sharkawy, I. ; Sherif, S. ; Mahboob, A. ; Abubaker, K. ; Bouzayen, M. ; Jayasankar, S. / Expression of auxin-binding protein1 during plum fruit ontogeny supports the potential role of auxin in initiating and enhancing climacteric ripening. In: Plant Cell Reports. 2012 ; Vol. 31, No. 10. pp. 1911-1921.
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