Abstract
Auxin-binding protein1 (ABP1) is an active element involved in auxin signaling and plays critical roles in auxin-mediated plant development. Here, we report the isolation and characterization of a putative sequence from Prunus salicina L., designated PslABP1. The expected protein exhibits a similar molecular structure to that of well-characterized maize-ABP1; however, PslABP1 displays more sequence polarity in the active-binding site due to substitution of some crucial amino-acid residues predicted to be involved in auxin-binding. Further, PslABP1 expression was assessed throughout fruit ontogeny to determine its role in fruit development. Comparing the expression data with the physiological aspects that characterize fruit-development stages indicates that PslABP1 up-regulation is usually associated with the signature events that are triggered in an auxin-dependent manner such as floral induction, fruit initiation, embryogenesis, and cell division and elongation. However, the diversity in PslABP1 expression profile during the ripening process of early and late plum cultivars seems to be due to the variability of endogenous auxin levels among the two cultivars, which consequently can change the levels of autocatalytic ethylene available for the fruit to co-ordinate ripening. The effect of auxin on stimulating ethylene production and in regulating PslABP1 was investigated. Our data suggest that auxin is involved in the transition of the mature green fruit into the ripening phase and in enhancing the ripening process in both auxin- and ethylene-dependent manners thereafter.
| Original language | English |
|---|---|
| Pages (from-to) | 1911-1921 |
| Number of pages | 11 |
| Journal | Plant Cell Reports |
| Volume | 31 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2012 |
| Externally published | Yes |
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Keywords
- Auxin
- Fruit development and ripening
- Protein structure
- Subcellular localization
ASJC Scopus subject areas
- Plant Science
- Agronomy and Crop Science
Cite this
Expression of auxin-binding protein1 during plum fruit ontogeny supports the potential role of auxin in initiating and enhancing climacteric ripening. / El-Sharkawy, I.; Sherif, S.; Mahboob, A.; Abubaker, K.; Bouzayen, M.; Jayasankar, S.
In: Plant Cell Reports, Vol. 31, No. 10, 10.2012, p. 1911-1921.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of auxin-binding protein1 during plum fruit ontogeny supports the potential role of auxin in initiating and enhancing climacteric ripening
AU - El-Sharkawy, I.
AU - Sherif, S.
AU - Mahboob, A.
AU - Abubaker, K.
AU - Bouzayen, M.
AU - Jayasankar, S.
PY - 2012/10
Y1 - 2012/10
N2 - Auxin-binding protein1 (ABP1) is an active element involved in auxin signaling and plays critical roles in auxin-mediated plant development. Here, we report the isolation and characterization of a putative sequence from Prunus salicina L., designated PslABP1. The expected protein exhibits a similar molecular structure to that of well-characterized maize-ABP1; however, PslABP1 displays more sequence polarity in the active-binding site due to substitution of some crucial amino-acid residues predicted to be involved in auxin-binding. Further, PslABP1 expression was assessed throughout fruit ontogeny to determine its role in fruit development. Comparing the expression data with the physiological aspects that characterize fruit-development stages indicates that PslABP1 up-regulation is usually associated with the signature events that are triggered in an auxin-dependent manner such as floral induction, fruit initiation, embryogenesis, and cell division and elongation. However, the diversity in PslABP1 expression profile during the ripening process of early and late plum cultivars seems to be due to the variability of endogenous auxin levels among the two cultivars, which consequently can change the levels of autocatalytic ethylene available for the fruit to co-ordinate ripening. The effect of auxin on stimulating ethylene production and in regulating PslABP1 was investigated. Our data suggest that auxin is involved in the transition of the mature green fruit into the ripening phase and in enhancing the ripening process in both auxin- and ethylene-dependent manners thereafter.
AB - Auxin-binding protein1 (ABP1) is an active element involved in auxin signaling and plays critical roles in auxin-mediated plant development. Here, we report the isolation and characterization of a putative sequence from Prunus salicina L., designated PslABP1. The expected protein exhibits a similar molecular structure to that of well-characterized maize-ABP1; however, PslABP1 displays more sequence polarity in the active-binding site due to substitution of some crucial amino-acid residues predicted to be involved in auxin-binding. Further, PslABP1 expression was assessed throughout fruit ontogeny to determine its role in fruit development. Comparing the expression data with the physiological aspects that characterize fruit-development stages indicates that PslABP1 up-regulation is usually associated with the signature events that are triggered in an auxin-dependent manner such as floral induction, fruit initiation, embryogenesis, and cell division and elongation. However, the diversity in PslABP1 expression profile during the ripening process of early and late plum cultivars seems to be due to the variability of endogenous auxin levels among the two cultivars, which consequently can change the levels of autocatalytic ethylene available for the fruit to co-ordinate ripening. The effect of auxin on stimulating ethylene production and in regulating PslABP1 was investigated. Our data suggest that auxin is involved in the transition of the mature green fruit into the ripening phase and in enhancing the ripening process in both auxin- and ethylene-dependent manners thereafter.
KW - Auxin
KW - Fruit development and ripening
KW - Protein structure
KW - Subcellular localization
UR - http://www.scopus.com/inward/record.url?scp=84866437231&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866437231&partnerID=8YFLogxK
U2 - 10.1007/s00299-012-1304-2
DO - 10.1007/s00299-012-1304-2
M3 - Article
C2 - 22739723
AN - SCOPUS:84866437231
VL - 31
SP - 1911
EP - 1921
JO - Plant Cell Reports
JF - Plant Cell Reports
SN - 0721-7714
IS - 10
ER -