TY - JOUR
T1 - Expression of early hematopoietic markers in cord and mobilized blood
AU - Panteleev, A. V.
AU - Vorobjev, I. A.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - G-CSF mobilized peripheral blood and cord blood are major sources of hematopoietic progenitor cells. These cells are characterized by expression of "early" antigens. We have evaluated the coexpression of hematopoietic markers CD34, CD133, CD90, CDCP1, and CD117 and activation antigen CD38 using multicolor flow cytometry. We showed that cells positive for each particular antigen generate a separate population. The percentage of cells expressing each particular "early" antigen is twice as high in the cord blood as in the mobilized blood. The cell number with complex progenitor phenotype (CD34+/CD38-/CD117-, CD133+/CD34+/CD38-, CDCP1+/CD34+/CD38-, etc.) is equal in mobilized and cord blood. There is a strong positive correlation between CD34, CD133, CD117, and CDCP1 expression in both groups. Positive correlation was observed between CD90 and CD34, CD133, CDCP1, and CD117 expression only in cord blood; it was not significant in mobilized blood. The analyses of early antigens' coexpression with activation CD38 marker did not confirm the hypothesis of sequential activation and loss of expression of the aforementioned antigens. We assume that there is global regulation of CD34, CD133, CDCP1, and CD117 expression. Expression of CD38 may be reversibly suppressed during maturation of the hematopoietic cells, and CD117 may be expressed on not only myeloid cells.
AB - G-CSF mobilized peripheral blood and cord blood are major sources of hematopoietic progenitor cells. These cells are characterized by expression of "early" antigens. We have evaluated the coexpression of hematopoietic markers CD34, CD133, CD90, CDCP1, and CD117 and activation antigen CD38 using multicolor flow cytometry. We showed that cells positive for each particular antigen generate a separate population. The percentage of cells expressing each particular "early" antigen is twice as high in the cord blood as in the mobilized blood. The cell number with complex progenitor phenotype (CD34+/CD38-/CD117-, CD133+/CD34+/CD38-, CDCP1+/CD34+/CD38-, etc.) is equal in mobilized and cord blood. There is a strong positive correlation between CD34, CD133, CD117, and CDCP1 expression in both groups. Positive correlation was observed between CD90 and CD34, CD133, CDCP1, and CD117 expression only in cord blood; it was not significant in mobilized blood. The analyses of early antigens' coexpression with activation CD38 marker did not confirm the hypothesis of sequential activation and loss of expression of the aforementioned antigens. We assume that there is global regulation of CD34, CD133, CDCP1, and CD117 expression. Expression of CD38 may be reversibly suppressed during maturation of the hematopoietic cells, and CD117 may be expressed on not only myeloid cells.
KW - CD117
KW - CD133
KW - CD34
KW - CDCP1
KW - G-CSF mobilized peripheral blood
KW - cord blood
KW - flow cytometry
KW - hematopoietic stem cell
UR - http://www.scopus.com/inward/record.url?scp=84874219506&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874219506&partnerID=8YFLogxK
U2 - 10.1134/S1990519X13010112
DO - 10.1134/S1990519X13010112
M3 - Article
AN - SCOPUS:84874219506
VL - 7
SP - 29
EP - 36
JO - Cell and Tissue Biology
JF - Cell and Tissue Biology
SN - 1990-519X
IS - 1
ER -