Expression of early hematopoietic markers in cord and mobilized blood

A. V. Panteleev, I. A. Vorobjev

Research output: Contribution to journalArticle

Abstract

G-CSF mobilized peripheral blood and cord blood are major sources of hematopoietic progenitor cells. These cells are characterized by expression of "early" antigens. We have evaluated the coexpression of hematopoietic markers CD34, CD133, CD90, CDCP1, and CD117 and activation antigen CD38 using multicolor flow cytometry. We showed that cells positive for each particular antigen generate a separate population. The percentage of cells expressing each particular "early" antigen is twice as high in the cord blood as in the mobilized blood. The cell number with complex progenitor phenotype (CD34+/CD38-/CD117-, CD133+/CD34+/CD38-, CDCP1+/CD34+/CD38-, etc.) is equal in mobilized and cord blood. There is a strong positive correlation between CD34, CD133, CD117, and CDCP1 expression in both groups. Positive correlation was observed between CD90 and CD34, CD133, CDCP1, and CD117 expression only in cord blood; it was not significant in mobilized blood. The analyses of early antigens' coexpression with activation CD38 marker did not confirm the hypothesis of sequential activation and loss of expression of the aforementioned antigens. We assume that there is global regulation of CD34, CD133, CDCP1, and CD117 expression. Expression of CD38 may be reversibly suppressed during maturation of the hematopoietic cells, and CD117 may be expressed on not only myeloid cells.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalCell and Tissue Biology
Volume7
Issue number1
DOIs
Publication statusPublished - Feb 27 2013

Keywords

  • CD117
  • CD133
  • CD34
  • CDCP1
  • G-CSF mobilized peripheral blood
  • cord blood
  • flow cytometry
  • hematopoietic stem cell

ASJC Scopus subject areas

  • Cell Biology

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