Abstract
We examined the expression of FGF-2 mRNA in 16 early and 14 advanced gastric cancer by in situ hybridization to elucidate its role in cancer progression. Anti-sense RNA probes were synthesized by transcribing the subcloned vector with T7 RNA polymerase in the presence of digoxigenin- labeled UTP. FGF-2 mRNA was located mainly in the cytoplasm around the nuclei of endothelial cells, fibroblasts and carcinoma cells. The expression was more frequently in the diffuse type carcinomas (4/7, 57%) than in the intestinal type tumours (5/23, 22%). The survival rates of advanced gastric cancers with FGF-2 mRNA expression were significantly lower than those without FGF-2 mRNA expression (p < 0.01). No significant correlation was seen with other clinicopathological factors. These results suggest that FGF-2 may play an important role for the growth of diffuse type gastric cancers, particularly at their advanced stage.
Original language | English |
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Pages (from-to) | 695-700 |
Number of pages | 6 |
Journal | Acta Oncologica |
Volume | 36 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1997 |
ASJC Scopus subject areas
- Hematology
- Oncology
- Radiology Nuclear Medicine and imaging