Abstract
We examined the expression of FGF-2 mRNA in 16 early and 14 advanced gastric cancer by in situ hybridization to elucidate its role in cancer progression. Anti-sense RNA probes were synthesized by transcribing the subcloned vector with T7 RNA polymerase in the presence of digoxigenin- labeled UTP. FGF-2 mRNA was located mainly in the cytoplasm around the nuclei of endothelial cells, fibroblasts and carcinoma cells. The expression was more frequently in the diffuse type carcinomas (4/7, 57%) than in the intestinal type tumours (5/23, 22%). The survival rates of advanced gastric cancers with FGF-2 mRNA expression were significantly lower than those without FGF-2 mRNA expression (p <0.01). No significant correlation was seen with other clinicopathological factors. These results suggest that FGF-2 may play an important role for the growth of diffuse type gastric cancers, particularly at their advanced stage.
| Original language | English |
|---|---|
| Pages (from-to) | 695-700 |
| Number of pages | 6 |
| Journal | Acta Oncologica |
| Volume | 36 |
| Issue number | 7 |
| Publication status | Published - 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Oncology
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Expression of fibroblast growth factor 2 mRNA in early and advanced gastric cancer. / Noda, Masao; Hattori, Takanori; Kimura, Tsuguhiro; Naitoh, Hiroyuki; Kodama, Tadashi; Kashima, Kei; Pignatelli, Massimo.
In: Acta Oncologica, Vol. 36, No. 7, 1997, p. 695-700.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of fibroblast growth factor 2 mRNA in early and advanced gastric cancer
AU - Noda, Masao
AU - Hattori, Takanori
AU - Kimura, Tsuguhiro
AU - Naitoh, Hiroyuki
AU - Kodama, Tadashi
AU - Kashima, Kei
AU - Pignatelli, Massimo
PY - 1997
Y1 - 1997
N2 - We examined the expression of FGF-2 mRNA in 16 early and 14 advanced gastric cancer by in situ hybridization to elucidate its role in cancer progression. Anti-sense RNA probes were synthesized by transcribing the subcloned vector with T7 RNA polymerase in the presence of digoxigenin- labeled UTP. FGF-2 mRNA was located mainly in the cytoplasm around the nuclei of endothelial cells, fibroblasts and carcinoma cells. The expression was more frequently in the diffuse type carcinomas (4/7, 57%) than in the intestinal type tumours (5/23, 22%). The survival rates of advanced gastric cancers with FGF-2 mRNA expression were significantly lower than those without FGF-2 mRNA expression (p <0.01). No significant correlation was seen with other clinicopathological factors. These results suggest that FGF-2 may play an important role for the growth of diffuse type gastric cancers, particularly at their advanced stage.
AB - We examined the expression of FGF-2 mRNA in 16 early and 14 advanced gastric cancer by in situ hybridization to elucidate its role in cancer progression. Anti-sense RNA probes were synthesized by transcribing the subcloned vector with T7 RNA polymerase in the presence of digoxigenin- labeled UTP. FGF-2 mRNA was located mainly in the cytoplasm around the nuclei of endothelial cells, fibroblasts and carcinoma cells. The expression was more frequently in the diffuse type carcinomas (4/7, 57%) than in the intestinal type tumours (5/23, 22%). The survival rates of advanced gastric cancers with FGF-2 mRNA expression were significantly lower than those without FGF-2 mRNA expression (p <0.01). No significant correlation was seen with other clinicopathological factors. These results suggest that FGF-2 may play an important role for the growth of diffuse type gastric cancers, particularly at their advanced stage.
UR - http://www.scopus.com/inward/record.url?scp=0031415992&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031415992&partnerID=8YFLogxK
M3 - Article
C2 - 9490085
AN - SCOPUS:0031415992
VL - 36
SP - 695
EP - 700
JO - Acta Oncologica
JF - Acta Oncologica
SN - 0284-186X
IS - 7
ER -