Expression of fibroblast growth factor 2 mRNA in early and advanced gastric cancer

Masao Noda; Takanori Hattori; Tsuguhiro Kimura; Hiroyuki Naitoh; Tadashi Kodama; Kei Kashima; Massimo Pignatelli

doi:10.3109/02841869709001339

Expression of fibroblast growth factor 2 mRNA in early and advanced gastric cancer

Masao Noda, Takanori Hattori, Tsuguhiro Kimura, Hiroyuki Naitoh, Tadashi Kodama, Kei Kashima, Massimo Pignatelli

School of Medicine

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We examined the expression of FGF-2 mRNA in 16 early and 14 advanced gastric cancer by in situ hybridization to elucidate its role in cancer progression. Anti-sense RNA probes were synthesized by transcribing the subcloned vector with T7 RNA polymerase in the presence of digoxigenin- labeled UTP. FGF-2 mRNA was located mainly in the cytoplasm around the nuclei of endothelial cells, fibroblasts and carcinoma cells. The expression was more frequently in the diffuse type carcinomas (4/7, 57%) than in the intestinal type tumours (5/23, 22%). The survival rates of advanced gastric cancers with FGF-2 mRNA expression were significantly lower than those without FGF-2 mRNA expression (p < 0.01). No significant correlation was seen with other clinicopathological factors. These results suggest that FGF-2 may play an important role for the growth of diffuse type gastric cancers, particularly at their advanced stage.

Original language	English
Pages (from-to)	695-700
Number of pages	6
Journal	Acta Oncologica
Volume	36
Issue number	7
DOIs	https://doi.org/10.3109/02841869709001339
Publication status	Published - 1997

ASJC Scopus subject areas

Hematology
Oncology
Radiology Nuclear Medicine and imaging

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title = "Expression of fibroblast growth factor 2 mRNA in early and advanced gastric cancer",

abstract = "We examined the expression of FGF-2 mRNA in 16 early and 14 advanced gastric cancer by in situ hybridization to elucidate its role in cancer progression. Anti-sense RNA probes were synthesized by transcribing the subcloned vector with T7 RNA polymerase in the presence of digoxigenin- labeled UTP. FGF-2 mRNA was located mainly in the cytoplasm around the nuclei of endothelial cells, fibroblasts and carcinoma cells. The expression was more frequently in the diffuse type carcinomas (4/7, 57%) than in the intestinal type tumours (5/23, 22%). The survival rates of advanced gastric cancers with FGF-2 mRNA expression were significantly lower than those without FGF-2 mRNA expression (p < 0.01). No significant correlation was seen with other clinicopathological factors. These results suggest that FGF-2 may play an important role for the growth of diffuse type gastric cancers, particularly at their advanced stage.",

author = "Masao Noda and Takanori Hattori and Tsuguhiro Kimura and Hiroyuki Naitoh and Tadashi Kodama and Kei Kashima and Massimo Pignatelli",

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T1 - Expression of fibroblast growth factor 2 mRNA in early and advanced gastric cancer

AU - Noda, Masao

AU - Hattori, Takanori

AU - Kimura, Tsuguhiro

AU - Naitoh, Hiroyuki

AU - Kodama, Tadashi

AU - Kashima, Kei

AU - Pignatelli, Massimo

PY - 1997

Y1 - 1997

N2 - We examined the expression of FGF-2 mRNA in 16 early and 14 advanced gastric cancer by in situ hybridization to elucidate its role in cancer progression. Anti-sense RNA probes were synthesized by transcribing the subcloned vector with T7 RNA polymerase in the presence of digoxigenin- labeled UTP. FGF-2 mRNA was located mainly in the cytoplasm around the nuclei of endothelial cells, fibroblasts and carcinoma cells. The expression was more frequently in the diffuse type carcinomas (4/7, 57%) than in the intestinal type tumours (5/23, 22%). The survival rates of advanced gastric cancers with FGF-2 mRNA expression were significantly lower than those without FGF-2 mRNA expression (p < 0.01). No significant correlation was seen with other clinicopathological factors. These results suggest that FGF-2 may play an important role for the growth of diffuse type gastric cancers, particularly at their advanced stage.

AB - We examined the expression of FGF-2 mRNA in 16 early and 14 advanced gastric cancer by in situ hybridization to elucidate its role in cancer progression. Anti-sense RNA probes were synthesized by transcribing the subcloned vector with T7 RNA polymerase in the presence of digoxigenin- labeled UTP. FGF-2 mRNA was located mainly in the cytoplasm around the nuclei of endothelial cells, fibroblasts and carcinoma cells. The expression was more frequently in the diffuse type carcinomas (4/7, 57%) than in the intestinal type tumours (5/23, 22%). The survival rates of advanced gastric cancers with FGF-2 mRNA expression were significantly lower than those without FGF-2 mRNA expression (p < 0.01). No significant correlation was seen with other clinicopathological factors. These results suggest that FGF-2 may play an important role for the growth of diffuse type gastric cancers, particularly at their advanced stage.

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