Expression of gelatinase A and TIMP-2 mRNAs in desmoplastic fibroblasts in both mammary carcinomas and basal cell carcinomas of the skin

R. Poulsom, A. M. Hanby, M. Pignatelli, R. E. Jeffery, J. M. Longcroft, L. Rogers, G. W H Stamp

Research output: Contribution to journalArticle

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Abstract

Aims - To compare the localisation of mRNAs for the basement membrane degrading enzyme gelatinase A (72 kilodalton type IV collagenase) and its inhibitor TIMP-2 in carcinomas of the breast and basal cell carcinomas of the skin which have little or no ability to metastasise. Methods - In situ hybridisation was performed on formalin fixed, paraffin wax embedded blocks using 35S-labelled riboprobes on 16 mammary carcinomas, three fibroadenomas, and a benign phyllodes tumour, and on 15 basal cell carcinomas of the skin (BCC). Results - Labelling for both mRNAs was detectable in 14 of 16 mammary carcinomas and in 13 of 15 BCC, most often over organising desmoplastic fibroblasts in the stroma around invasive epithelial aggregates. Some sparse labelling was seen over malignant epithelial cells in six of the mammary carcinomas but not in the BCC. Some expression of gelatinase A mRNA was also seen in fibroblasts of breast lobules adjacent to the mammary carcinomas and around engulfed adnexal elements in the BCC, but not in unaffected breast tissues, fibroadenomas, the phyllodes tumour or unaffected skin. Conclusions - Maximal expression of gelatinase A and TIMP-2 mRNAs occurs in malignant neoplasms as part of the host response to the presence of established neoplastic cells rather than as an initial response to invasion. The degree to which this is present suggests this may be a highly relevant mechanism modulating tumour differentiation, growth and progression, possibly entailing uptake via specific receptors on the tumour cell surface.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalJournal of Clinical Pathology
Volume46
Issue number5
Publication statusPublished - 1993
Externally publishedYes

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Tissue Inhibitor of Metalloproteinase-2
Basal Cell Carcinoma
Matrix Metalloproteinase 2
Fibroblasts
Breast Neoplasms
Messenger RNA
Skin
Phyllodes Tumor
Fibroadenoma
Breast
Neoplasms
Matrix Metalloproteinase Inhibitors
Waxes
Basement Membrane
Paraffin
Formaldehyde
In Situ Hybridization
Epithelial Cells
Enzymes
Growth

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Expression of gelatinase A and TIMP-2 mRNAs in desmoplastic fibroblasts in both mammary carcinomas and basal cell carcinomas of the skin. / Poulsom, R.; Hanby, A. M.; Pignatelli, M.; Jeffery, R. E.; Longcroft, J. M.; Rogers, L.; Stamp, G. W H.

In: Journal of Clinical Pathology, Vol. 46, No. 5, 1993, p. 429-436.

Research output: Contribution to journalArticle

Poulsom, R. ; Hanby, A. M. ; Pignatelli, M. ; Jeffery, R. E. ; Longcroft, J. M. ; Rogers, L. ; Stamp, G. W H. / Expression of gelatinase A and TIMP-2 mRNAs in desmoplastic fibroblasts in both mammary carcinomas and basal cell carcinomas of the skin. In: Journal of Clinical Pathology. 1993 ; Vol. 46, No. 5. pp. 429-436.
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T1 - Expression of gelatinase A and TIMP-2 mRNAs in desmoplastic fibroblasts in both mammary carcinomas and basal cell carcinomas of the skin

AU - Poulsom, R.

AU - Hanby, A. M.

AU - Pignatelli, M.

AU - Jeffery, R. E.

AU - Longcroft, J. M.

AU - Rogers, L.

AU - Stamp, G. W H

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