Fe₃O₄ Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy

Kanat Dukenbayev, Ilya V. Korolkov, Daria I. Tishkevich, Artem L. Kozlovskiy, Sergey V. Trukhanov, Yevgeniy G. Gorin, Elena E. Shumskaya, Egor Y. Kaniukov, Denis A. Vinnik, Maxim V. Zdorovets, Marina Anisovich, Alex V. Trukhanov, Daniele Tosi, Carlo Molardi

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Magnetic Fe 3 O 4 nanoparticles (NPs) and their surface modification with therapeutic substances are of great interest, especially drug delivery for cancer therapy, including boron-neutron capture therapy (BNCT). In this paper, we present the results of boron-rich compound (carborane borate) attachment to previously aminated by (3-aminopropyl)-trimethoxysilane (APTMS) iron oxide NPs. Fourier transform infrared spectroscopy with Attenuated total reflectance accessory (ATR-FTIR) and energy-dispersive X-ray analysis confirmed the change of the element content of NPs after modification and formation of new bonds between Fe3O4 NPs and the attached molecules. Transmission (TEM) and scanning electron microscopy (SEM) showed Fe3O4 NPs’ average size of 18.9 nm. Phase parameters were studied by powder X-ray diffraction (XRD), and the magnetic behavior of Fe 3 O 4 NPs was elucidated by Mössbauer spectroscopy. The colloidal and chemical stability of NPs was studied using simulated body fluid (phosphate buffer-PBS). Modified NPs have shown excellent stability in PBS (pH = 7.4), characterized by XRD, Mössbauer spectroscopy, and dynamic light scattering (DLS). Biocompatibility was evaluated in-vitro using cultured mouse embryonic fibroblasts (MEFs). The results show us an increasing of IC50 from 0.110 mg/mL for Fe 3 O 4 NPs to 0.405 mg/mL for Fe 3 O 4 -Carborane NPs. The obtained data confirm the biocompatibility and stability of synthesized NPs and the potential to use them in BNCT.

Original languageEnglish
Article number494
JournalNanomaterials
Volume9
Issue number4
DOIs
Publication statusPublished - Apr 1 2019

Keywords

  • APTMS
  • Carborane
  • Iron oxide
  • Magnetic nanoparticles
  • Surface functionalization

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

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