Fibronectin-Alginate microcapsules improve cell viability and protein secretion of encapsulated Factor IX-engineered human mesenchymal stromal cells

Bahareh Sayyar, Megan Dodd, Leah Marquez-Curtis, Anna Janowska-Wieczorek, Gonzalo Hortelano

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Continuous delivery of proteins by engineered cells encapsulated in biocompatible polymeric microcapsules is of considerable therapeutic potential. However, this technology has not lived up to expectations due to inadequate cell - matrix interactions and subsequent cell death. In this study we hypothesize that the presence of fibronectin in an alginate matrix may enhance the viability and functionality of encapsulated human cord blood-derived mesenchymal stromal cells (MSCs) expressing the human Factor IX (FIX) gene. MSCs were encapsulated in alginate-PLL microcapsules containing 10, 100, or 500 μ g/ml fibronectin to ameliorate cell survival. MSCs in microcapsules with 100 and 500 μ g/ml fibronectin demonstrated improved cell viability and proliferation and higher FIX secretion compared to MSCs in non-supplemented microcapsules. In contrast, 10 μ g/ml fibronectin did not significantly affect the viability and protein secretion from the encapsulated cells. Differentiation studies demonstrated osteogenic (but not chondrogenic or adipogenic) differentiation capability and efficient FIX secretion of the enclosed MSCs in the fibronectin-alginate suspension culture. Thus, the use of recombinant MSCs encapsulated in fibronectin-alginate microcapsules in basal or osteogenic cultures may be of practical use in the treatment of hemophilia B.

Original languageEnglish
Pages (from-to)318-327
Number of pages10
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume43
Issue number5
DOIs
Publication statusPublished - Jan 1 2015

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Keywords

  • Alginate
  • Bioactivity
  • Cell encapsulation
  • Factor IX
  • Fibronectin
  • Hemophilia B
  • Mesenchymal stromal cells

ASJC Scopus subject areas

  • Biotechnology
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science

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