Flow fluorimetry in differential diagnosis of diffuse large B-cell lymphoma

I. A. Vorobyev; L. A. Gorgidze; E. M. Gretsov; E. P. Korneva; D. V. Kharazishvili; O. A. Khudoleeva; Zh V. Churakova

Flow fluorimetry in differential diagnosis of diffuse large B-cell lymphoma

I. A. Vorobyev, L. A. Gorgidze, E. M. Gretsov, E. P. Korneva, D. V. Kharazishvili, O. A. Khudoleeva, Zh V. Churakova

Department of Biology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Aim. To analyse immunophenotype of diffuse large B-cell lymphoma (DLBCL) with flow cytometry. Material and methods. Combinations of antibodies against the following antigens were used: CD3/CD19/CD45, CD5/CD19/CD38, CD19/CD10/CD23, CD4/CD8/CD3, cappa/lambda/CD19, CD25/CD20/FMC7; CD43/CD22/CD20; CD79a/Ki-67/CD3; cytoplasmic cappa/lambda. The analysis was made on flow fluorimeter FacsCalibur using computer program CellQuest (Beckton Dickenson, USA). Results. Specific coexpression of markers is not detectable in DLBCL, in the greatest degree the phenotype corresponds to lymphoma from the cells of the marginal zone. The study of cells with maximal direct light diffusion provides more precise assessment of clonality and proliferative potential of tumor cells than the analysis of the whole lymphocytic polygon. The proliferative index in 33 cases of DLBCL varied in the range 10-60%. Conclusion. Flow cytometry in most DLBCL cases allows identification of B-cell clonality, more precise assessment of a proliferative potential of the tumor.

Original language	English
Pages (from-to)	46-51
Number of pages	6
Journal	Terapevticheskii Arkhiv
Volume	78
Issue number	7
Publication status	Published - Aug 30 2006

Keywords

Diagnosis
Diffuse large B-cell lymphoma
Flow fluorimetry
Immunophenotype

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism

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@article{4840299515704998b7d99ad973119896,

title = "Flow fluorimetry in differential diagnosis of diffuse large B-cell lymphoma",

abstract = "Aim. To analyse immunophenotype of diffuse large B-cell lymphoma (DLBCL) with flow cytometry. Material and methods. Combinations of antibodies against the following antigens were used: CD3/CD19/CD45, CD5/CD19/CD38, CD19/CD10/CD23, CD4/CD8/CD3, cappa/lambda/CD19, CD25/CD20/FMC7; CD43/CD22/CD20; CD79a/Ki-67/CD3; cytoplasmic cappa/lambda. The analysis was made on flow fluorimeter FacsCalibur using computer program CellQuest (Beckton Dickenson, USA). Results. Specific coexpression of markers is not detectable in DLBCL, in the greatest degree the phenotype corresponds to lymphoma from the cells of the marginal zone. The study of cells with maximal direct light diffusion provides more precise assessment of clonality and proliferative potential of tumor cells than the analysis of the whole lymphocytic polygon. The proliferative index in 33 cases of DLBCL varied in the range 10-60%. Conclusion. Flow cytometry in most DLBCL cases allows identification of B-cell clonality, more precise assessment of a proliferative potential of the tumor.",

keywords = "Diagnosis, Diffuse large B-cell lymphoma, Flow fluorimetry, Immunophenotype",

author = "Vorobyev, {I. A.} and Gorgidze, {L. A.} and Gretsov, {E. M.} and Korneva, {E. P.} and Kharazishvili, {D. V.} and Khudoleeva, {O. A.} and Churakova, {Zh V.}",

year = "2006",

month = aug,

day = "30",

language = "English",

volume = "78",

pages = "46--51",

journal = "Terapevticheskii Arkhiv",

issn = "0040-3660",

publisher = "Izdatel'stvo Meditsina",

number = "7",

}

TY - JOUR

T1 - Flow fluorimetry in differential diagnosis of diffuse large B-cell lymphoma

AU - Vorobyev, I. A.

AU - Gorgidze, L. A.

AU - Gretsov, E. M.

AU - Korneva, E. P.

AU - Kharazishvili, D. V.

AU - Khudoleeva, O. A.

AU - Churakova, Zh V.

PY - 2006/8/30

Y1 - 2006/8/30

N2 - Aim. To analyse immunophenotype of diffuse large B-cell lymphoma (DLBCL) with flow cytometry. Material and methods. Combinations of antibodies against the following antigens were used: CD3/CD19/CD45, CD5/CD19/CD38, CD19/CD10/CD23, CD4/CD8/CD3, cappa/lambda/CD19, CD25/CD20/FMC7; CD43/CD22/CD20; CD79a/Ki-67/CD3; cytoplasmic cappa/lambda. The analysis was made on flow fluorimeter FacsCalibur using computer program CellQuest (Beckton Dickenson, USA). Results. Specific coexpression of markers is not detectable in DLBCL, in the greatest degree the phenotype corresponds to lymphoma from the cells of the marginal zone. The study of cells with maximal direct light diffusion provides more precise assessment of clonality and proliferative potential of tumor cells than the analysis of the whole lymphocytic polygon. The proliferative index in 33 cases of DLBCL varied in the range 10-60%. Conclusion. Flow cytometry in most DLBCL cases allows identification of B-cell clonality, more precise assessment of a proliferative potential of the tumor.

AB - Aim. To analyse immunophenotype of diffuse large B-cell lymphoma (DLBCL) with flow cytometry. Material and methods. Combinations of antibodies against the following antigens were used: CD3/CD19/CD45, CD5/CD19/CD38, CD19/CD10/CD23, CD4/CD8/CD3, cappa/lambda/CD19, CD25/CD20/FMC7; CD43/CD22/CD20; CD79a/Ki-67/CD3; cytoplasmic cappa/lambda. The analysis was made on flow fluorimeter FacsCalibur using computer program CellQuest (Beckton Dickenson, USA). Results. Specific coexpression of markers is not detectable in DLBCL, in the greatest degree the phenotype corresponds to lymphoma from the cells of the marginal zone. The study of cells with maximal direct light diffusion provides more precise assessment of clonality and proliferative potential of tumor cells than the analysis of the whole lymphocytic polygon. The proliferative index in 33 cases of DLBCL varied in the range 10-60%. Conclusion. Flow cytometry in most DLBCL cases allows identification of B-cell clonality, more precise assessment of a proliferative potential of the tumor.

KW - Diagnosis

KW - Diffuse large B-cell lymphoma

KW - Flow fluorimetry

KW - Immunophenotype

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