Fluorescent semiconductor nanocrystals in microscopy and flow cytometry

I. A. Vorobjev, E. P. Rafalovskaya-Orlovskaya, A. A. Gladkih, D. M. Potashnikova, N. S. Barteneva

Research output: Contribution to journalReview article

Abstract

Quantum dots nanocrystals (Qdots or QDs), consisting of a CdSe core with a ZnS shell, are a novel class of fluorochromes with significant advantages over traditional organic fluorochromes and fluorescent proteins. QDs have a large extinction coefficient, high photostability, wide absorption and narrow emission spectra, and large Stokes shifts. These features make them desirable for both microscopy and flow cytometry. Applications of QD-conjugates with antibodies, streptavidin, and DNA or RNA probes have made it possible to produce highly stable multicolor specimens useful for scientific and diagnostic purposes. The current review describes the achievements in preparation of multicolor specimens based on QD-conjugates for microscopy and flow cytometry and outlines the requirements for microscope and flow cytometer reengineering for successful analysis of these specimens. However, despite considerable progress, two of the obstacles that preclude wider use of QDs include some of their chemical properties and the large size of QD-conjugates. Difficulties in the application of QDs are similar whether commercial or custom-made conjugates are used.

Original languageEnglish
Pages (from-to)321-331
Number of pages11
JournalCell and Tissue Biology
Volume5
Issue number4
DOIs
Publication statusPublished - Aug 2011
Externally publishedYes

Fingerprint

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Keywords

  • flow-cytometry
  • fluorescent nanocristalls
  • immunohystochemistry
  • quantum dots

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fluorescent semiconductor nanocrystals in microscopy and flow cytometry. / Vorobjev, I. A.; Rafalovskaya-Orlovskaya, E. P.; Gladkih, A. A.; Potashnikova, D. M.; Barteneva, N. S.

In: Cell and Tissue Biology, Vol. 5, No. 4, 08.2011, p. 321-331.

Research output: Contribution to journalReview article

Vorobjev, I. A. ; Rafalovskaya-Orlovskaya, E. P. ; Gladkih, A. A. ; Potashnikova, D. M. ; Barteneva, N. S. / Fluorescent semiconductor nanocrystals in microscopy and flow cytometry. In: Cell and Tissue Biology. 2011 ; Vol. 5, No. 4. pp. 321-331.
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