Focal adhesion disassembly requires clathrin-dependent endocytosis of integrins

Wei Ting Chao, Jeannette Kunz

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Cell migration requires the controlled disassembly of focal adhesions, but the underlying mechanisms remain poorly understood. Here, we show that adhesion turnover is mediated through dynamin- and clathrin-dependent endocytosis of activated β1 integrins. Consistent with this, clathrin and the clathrin adaptors AP-2 and disabled-2 (DAB2) distribute along with dynamin 2 to adhesion sites prior to adhesion disassembly. Moreover, knockdown of either dynamin 2 or both clathrin adaptors blocks β1 integrin internalization, leading to impaired focal adhesion disassembly and cell migration. Together, these results provide important insight into the mechanisms underlying adhesion disassembly and identify novel components of the disassembly pathway.

Original languageEnglish
Pages (from-to)1337-1343
Number of pages7
JournalFEBS Letters
Volume583
Issue number8
DOIs
Publication statusPublished - Apr 17 2009
Externally publishedYes

Fingerprint

Dynamin II
Vesicular Transport Adaptor Proteins
Clathrin
Focal Adhesions
Endocytosis
Integrins
Cell Movement
Adhesion
Dynamins

Keywords

  • Clathrin
  • DAB2
  • Dynamin
  • Endocytosis
  • Focal adhesion disassembly

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Focal adhesion disassembly requires clathrin-dependent endocytosis of integrins. / Chao, Wei Ting; Kunz, Jeannette.

In: FEBS Letters, Vol. 583, No. 8, 17.04.2009, p. 1337-1343.

Research output: Contribution to journalArticle

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