Focal adhesion disassembly requires clathrin-dependent endocytosis of integrins

Wei Ting Chao, Jeannette Kunz

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Cell migration requires the controlled disassembly of focal adhesions, but the underlying mechanisms remain poorly understood. Here, we show that adhesion turnover is mediated through dynamin- and clathrin-dependent endocytosis of activated β1 integrins. Consistent with this, clathrin and the clathrin adaptors AP-2 and disabled-2 (DAB2) distribute along with dynamin 2 to adhesion sites prior to adhesion disassembly. Moreover, knockdown of either dynamin 2 or both clathrin adaptors blocks β1 integrin internalization, leading to impaired focal adhesion disassembly and cell migration. Together, these results provide important insight into the mechanisms underlying adhesion disassembly and identify novel components of the disassembly pathway.

Original languageEnglish
Pages (from-to)1337-1343
Number of pages7
JournalFEBS Letters
Volume583
Issue number8
DOIs
Publication statusPublished - Apr 17 2009

Keywords

  • Clathrin
  • DAB2
  • Dynamin
  • Endocytosis
  • Focal adhesion disassembly

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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