Formation of macroporous self-assembled hydrogels through cryogelation of Fmoc-Phe-Phe

Dmitriy Berillo; Bo Mattiasson; Igor Yu Galaev; Harald Kirsebom

doi:10.1016/j.jcis.2011.11.006

Formation of macroporous self-assembled hydrogels through cryogelation of Fmoc-Phe-Phe

Dmitriy Berillo, Bo Mattiasson, Igor Yu Galaev, Harald Kirsebom

National Laboratory Astana

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

In this study, it was found that macroporous hydrogels were formed when self-assembly of fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) peptides was induced using glucono-δ-lactone (GdL) in apparently frozen samples. Formed cryogels exhibited a heterogeneous structure with pore walls of densely packed fibres of assembled dipeptides and pores in the range 10-100μm. Hydrogels formed from the same composition above the freezing point exhibited a homogenous structure without any apparent porosity. The formed gels were characterised using microscopy techniques, CD-spectroscopy and stress sweeps. The cryogels exhibited less mechanical strength than the hydrogels that might be due to the heterogeneous structure of the former. It appeared that the self-assembled peptide both in the cryo- and hydrogel maintained the β-sheet structure commonly attributed to these.

Original language	English
Pages (from-to)	226-230
Number of pages	5
Journal	Journal of Colloid and Interface Science
Volume	368
Issue number	1
DOIs	https://doi.org/10.1016/j.jcis.2011.11.006
Publication status	Published - Feb 15 2012

Keywords

Dipeptides
Hydrogels
Macroporous
Peptides
Self-assembly

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Surfaces, Coatings and Films
Colloid and Surface Chemistry

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title = "Formation of macroporous self-assembled hydrogels through cryogelation of Fmoc-Phe-Phe",

abstract = "In this study, it was found that macroporous hydrogels were formed when self-assembly of fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) peptides was induced using glucono-δ-lactone (GdL) in apparently frozen samples. Formed cryogels exhibited a heterogeneous structure with pore walls of densely packed fibres of assembled dipeptides and pores in the range 10-100μm. Hydrogels formed from the same composition above the freezing point exhibited a homogenous structure without any apparent porosity. The formed gels were characterised using microscopy techniques, CD-spectroscopy and stress sweeps. The cryogels exhibited less mechanical strength than the hydrogels that might be due to the heterogeneous structure of the former. It appeared that the self-assembled peptide both in the cryo- and hydrogel maintained the β-sheet structure commonly attributed to these.",

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AU - Galaev, Igor Yu

AU - Kirsebom, Harald

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