Formation of macroporous self-assembled hydrogels through cryogelation of Fmoc-Phe-Phe

Dmitriy Berillo, Bo Mattiasson, Igor Yu Galaev, Harald Kirsebom

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

In this study, it was found that macroporous hydrogels were formed when self-assembly of fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) peptides was induced using glucono-δ-lactone (GdL) in apparently frozen samples. Formed cryogels exhibited a heterogeneous structure with pore walls of densely packed fibres of assembled dipeptides and pores in the range 10-100μm. Hydrogels formed from the same composition above the freezing point exhibited a homogenous structure without any apparent porosity. The formed gels were characterised using microscopy techniques, CD-spectroscopy and stress sweeps. The cryogels exhibited less mechanical strength than the hydrogels that might be due to the heterogeneous structure of the former. It appeared that the self-assembled peptide both in the cryo- and hydrogel maintained the β-sheet structure commonly attributed to these.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalJournal of Colloid and Interface Science
Volume368
Issue number1
DOIs
Publication statusPublished - Feb 15 2012
Externally publishedYes

Fingerprint

Hydrogels
Cryogels
Peptides
Dipeptides
Hydrogel
Lactones
Freezing
Self assembly
Strength of materials
Microscopic examination
Porosity
Gels
Spectroscopy
Fibers
Chemical analysis

Keywords

  • Dipeptides
  • Hydrogels
  • Macroporous
  • Peptides
  • Self-assembly

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Colloid and Surface Chemistry

Cite this

Formation of macroporous self-assembled hydrogels through cryogelation of Fmoc-Phe-Phe. / Berillo, Dmitriy; Mattiasson, Bo; Galaev, Igor Yu; Kirsebom, Harald.

In: Journal of Colloid and Interface Science, Vol. 368, No. 1, 15.02.2012, p. 226-230.

Research output: Contribution to journalArticle

Berillo, Dmitriy ; Mattiasson, Bo ; Galaev, Igor Yu ; Kirsebom, Harald. / Formation of macroporous self-assembled hydrogels through cryogelation of Fmoc-Phe-Phe. In: Journal of Colloid and Interface Science. 2012 ; Vol. 368, No. 1. pp. 226-230.
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