Functionalized etched tilted fiber Bragg grating aptasensor for label-free protein detection

Marzhan Sypabekova, Sanzhar Korganbayev, Álvaro González-Vila, Christophe Caucheteur, Madina Shaimerdenova, Takhmina Ayupova, Aliya Bekmurzayeva, Luca Vangelista, Daniele Tosi

Research output: Contribution to journalArticle

Abstract

An aptasensor based on etched tilted fiber Bragg grating (eTFBG) is developed on a single-mode optical fiber targeting biomolecule detection. TFBGs were chemically etched using hydrofluoric acid (HF) to partially remove the fiber cladding. The sensor response was coarsely interrogated, resulting on a sensitivity increase from 1.25 nm/RIU (refractive index unit) at the beginning of the process, up to 23.38 nm/RIU at the end of the etching, for a RI range from 1.3418 to 1.4419 RIU. The proposed aptasensor showed improved RI sensitivity as compared to the unetched TFBG, without requiring metal depositions on the fiber surface or polarization control during the measurements. The proposed sensor was tested for the detection of thrombin-aptamer interactions based on silane-coupling surface chemistry, with thrombin concentrations ranging from 2.5 to 40 nM. Functionalized eTFBGs provided a competitive platform for biochemical interaction measurements, showing sensitivity values ranging from 2.3 to 3.3 p.m./nM for the particular case of thrombin detection.

Original languageEnglish
Article number111765
JournalBiosensors and Bioelectronics
Volume146
DOIs
Publication statusPublished - Dec 15 2019

Fingerprint

Fiber Bragg gratings
Thrombin
Labels
Hydrofluoric Acid
Proteins
Optical Fibers
Silanes
Refractometry
Hydrofluoric acid
Fibers
Sensors
Biomolecules
Single mode fibers
Surface chemistry
Optical fibers
Etching
Refractive index
Metals
Polarization
thrombin aptamer

Keywords

  • Aptasensor
  • Detection
  • Etching
  • Optical fiber
  • Thrombin

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Functionalized etched tilted fiber Bragg grating aptasensor for label-free protein detection. / Sypabekova, Marzhan; Korganbayev, Sanzhar; González-Vila, Álvaro; Caucheteur, Christophe; Shaimerdenova, Madina; Ayupova, Takhmina; Bekmurzayeva, Aliya; Vangelista, Luca; Tosi, Daniele.

In: Biosensors and Bioelectronics , Vol. 146, 111765, 15.12.2019.

Research output: Contribution to journalArticle

Sypabekova, Marzhan ; Korganbayev, Sanzhar ; González-Vila, Álvaro ; Caucheteur, Christophe ; Shaimerdenova, Madina ; Ayupova, Takhmina ; Bekmurzayeva, Aliya ; Vangelista, Luca ; Tosi, Daniele. / Functionalized etched tilted fiber Bragg grating aptasensor for label-free protein detection. In: Biosensors and Bioelectronics . 2019 ; Vol. 146.
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