TY - JOUR
T1 - Functionalized etched tilted fiber Bragg grating aptasensor for label-free protein detection
AU - Sypabekova, Marzhan
AU - Korganbayev, Sanzhar
AU - González-Vila, Álvaro
AU - Caucheteur, Christophe
AU - Shaimerdenova, Madina
AU - Ayupova, Takhmina
AU - Bekmurzayeva, Aliya
AU - Vangelista, Luca
AU - Tosi, Daniele
N1 - Funding Information:
This work has been funded by ORAU program at Nazarbayev University (grant: LIFESTART ; PI: Daniele Tosi ). The research has been supported by NATO Science for Peace and Security program (grant G5486 ). Álvaro González-Vila and Christophe Caucheteur are supported by the Belgian F.R.S.-FNRS , also through the grant no. O001518F (EOS-convention 30467715).
Funding Information:
This work has been funded by ORAU program at Nazarbayev University (grant: LIFESTART; PI: Daniele Tosi). The research has been supported by NATO Science for Peace and Security program (grant G5486). Álvaro González-Vila and Christophe Caucheteur are supported by the Belgian F.R.S.-FNRS, also through the grant no. O001518F (EOS-convention 30467715).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/12/15
Y1 - 2019/12/15
N2 - An aptasensor based on etched tilted fiber Bragg grating (eTFBG) is developed on a single-mode optical fiber targeting biomolecule detection. TFBGs were chemically etched using hydrofluoric acid (HF) to partially remove the fiber cladding. The sensor response was coarsely interrogated, resulting on a sensitivity increase from 1.25 nm/RIU (refractive index unit) at the beginning of the process, up to 23.38 nm/RIU at the end of the etching, for a RI range from 1.3418 to 1.4419 RIU. The proposed aptasensor showed improved RI sensitivity as compared to the unetched TFBG, without requiring metal depositions on the fiber surface or polarization control during the measurements. The proposed sensor was tested for the detection of thrombin-aptamer interactions based on silane-coupling surface chemistry, with thrombin concentrations ranging from 2.5 to 40 nM. Functionalized eTFBGs provided a competitive platform for biochemical interaction measurements, showing sensitivity values ranging from 2.3 to 3.3 p.m./nM for the particular case of thrombin detection.
AB - An aptasensor based on etched tilted fiber Bragg grating (eTFBG) is developed on a single-mode optical fiber targeting biomolecule detection. TFBGs were chemically etched using hydrofluoric acid (HF) to partially remove the fiber cladding. The sensor response was coarsely interrogated, resulting on a sensitivity increase from 1.25 nm/RIU (refractive index unit) at the beginning of the process, up to 23.38 nm/RIU at the end of the etching, for a RI range from 1.3418 to 1.4419 RIU. The proposed aptasensor showed improved RI sensitivity as compared to the unetched TFBG, without requiring metal depositions on the fiber surface or polarization control during the measurements. The proposed sensor was tested for the detection of thrombin-aptamer interactions based on silane-coupling surface chemistry, with thrombin concentrations ranging from 2.5 to 40 nM. Functionalized eTFBGs provided a competitive platform for biochemical interaction measurements, showing sensitivity values ranging from 2.3 to 3.3 p.m./nM for the particular case of thrombin detection.
KW - Aptasensor
KW - Detection
KW - Etching
KW - Optical fiber
KW - Thrombin
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U2 - 10.1016/j.bios.2019.111765
DO - 10.1016/j.bios.2019.111765
M3 - Article
C2 - 31606689
AN - SCOPUS:85072972118
SN - 0956-5663
VL - 146
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 111765
ER -