Further evaluation of immunocytochemical staining in the diagnosis of early myocardial ischaemic/hypoxic damage

Stephen Leadbeatter, Helen M. Wawman, Bharat Jasani

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The pattern of immunocytochemical staining with antibodies to caeruloplasmin, myosin, myoglobin and C-reactive protein seen in myocardium taken from deaths with macroscopic evidence of myocardial infarction and/or significant coronary artery atherosclerosis and from deaths with neither of these lesions has been correlated with H&E, PTAH and HBFP staining of myocardium and circumstances of each death indicative of antemortem hypoxia and/or ischaemia. Loss of staining with these antibodies correlated well with fuchsinorrhagia and both techniques are more sensitive than H&E and PTAH staining in the detection of early ischaemic/hypoxic damage to myocardium. However, their sensitivity is such that they appear to detect agonal changes and, therefore, cannot be used for specific diagnosis of early myocardial infarction.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalForensic Science International
Volume45
Issue number1-2
DOIs
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Staining and Labeling
Myocardium
Ceruloplasmin
Antibodies
Myoglobin
Myosins
C-Reactive Protein
Myocardial Infarction
Coronary Artery Disease
Coronary Vessels
Ischemia
Hypoxia

Keywords

  • Immunoperoxidase staining
  • Ischaemic/hypoxic damage
  • Myocardium

ASJC Scopus subject areas

  • Genetics
  • Analytical Chemistry
  • Pathology and Forensic Medicine
  • Psychiatry and Mental health

Cite this

Further evaluation of immunocytochemical staining in the diagnosis of early myocardial ischaemic/hypoxic damage. / Leadbeatter, Stephen; Wawman, Helen M.; Jasani, Bharat.

In: Forensic Science International, Vol. 45, No. 1-2, 1990, p. 135-141.

Research output: Contribution to journalArticle

Leadbeatter, Stephen ; Wawman, Helen M. ; Jasani, Bharat. / Further evaluation of immunocytochemical staining in the diagnosis of early myocardial ischaemic/hypoxic damage. In: Forensic Science International. 1990 ; Vol. 45, No. 1-2. pp. 135-141.
@article{e88b407598eb4a0d8470e7c47bbf437b,
title = "Further evaluation of immunocytochemical staining in the diagnosis of early myocardial ischaemic/hypoxic damage",
abstract = "The pattern of immunocytochemical staining with antibodies to caeruloplasmin, myosin, myoglobin and C-reactive protein seen in myocardium taken from deaths with macroscopic evidence of myocardial infarction and/or significant coronary artery atherosclerosis and from deaths with neither of these lesions has been correlated with H&E, PTAH and HBFP staining of myocardium and circumstances of each death indicative of antemortem hypoxia and/or ischaemia. Loss of staining with these antibodies correlated well with fuchsinorrhagia and both techniques are more sensitive than H&E and PTAH staining in the detection of early ischaemic/hypoxic damage to myocardium. However, their sensitivity is such that they appear to detect agonal changes and, therefore, cannot be used for specific diagnosis of early myocardial infarction.",
keywords = "Immunoperoxidase staining, Ischaemic/hypoxic damage, Myocardium",
author = "Stephen Leadbeatter and Wawman, {Helen M.} and Bharat Jasani",
year = "1990",
doi = "10.1016/0379-0738(90)90230-V",
language = "English",
volume = "45",
pages = "135--141",
journal = "Forensic Science International",
issn = "0379-0738",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Further evaluation of immunocytochemical staining in the diagnosis of early myocardial ischaemic/hypoxic damage

AU - Leadbeatter, Stephen

AU - Wawman, Helen M.

AU - Jasani, Bharat

PY - 1990

Y1 - 1990

N2 - The pattern of immunocytochemical staining with antibodies to caeruloplasmin, myosin, myoglobin and C-reactive protein seen in myocardium taken from deaths with macroscopic evidence of myocardial infarction and/or significant coronary artery atherosclerosis and from deaths with neither of these lesions has been correlated with H&E, PTAH and HBFP staining of myocardium and circumstances of each death indicative of antemortem hypoxia and/or ischaemia. Loss of staining with these antibodies correlated well with fuchsinorrhagia and both techniques are more sensitive than H&E and PTAH staining in the detection of early ischaemic/hypoxic damage to myocardium. However, their sensitivity is such that they appear to detect agonal changes and, therefore, cannot be used for specific diagnosis of early myocardial infarction.

AB - The pattern of immunocytochemical staining with antibodies to caeruloplasmin, myosin, myoglobin and C-reactive protein seen in myocardium taken from deaths with macroscopic evidence of myocardial infarction and/or significant coronary artery atherosclerosis and from deaths with neither of these lesions has been correlated with H&E, PTAH and HBFP staining of myocardium and circumstances of each death indicative of antemortem hypoxia and/or ischaemia. Loss of staining with these antibodies correlated well with fuchsinorrhagia and both techniques are more sensitive than H&E and PTAH staining in the detection of early ischaemic/hypoxic damage to myocardium. However, their sensitivity is such that they appear to detect agonal changes and, therefore, cannot be used for specific diagnosis of early myocardial infarction.

KW - Immunoperoxidase staining

KW - Ischaemic/hypoxic damage

KW - Myocardium

UR - http://www.scopus.com/inward/record.url?scp=0025228388&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025228388&partnerID=8YFLogxK

U2 - 10.1016/0379-0738(90)90230-V

DO - 10.1016/0379-0738(90)90230-V

M3 - Article

VL - 45

SP - 135

EP - 141

JO - Forensic Science International

JF - Forensic Science International

SN - 0379-0738

IS - 1-2

ER -