GABAA receptor inhibition does not affect mGluR-dependent LTD at hippocampal Schaffer collateral-CA1 synapses

Marco Rohde, Tursonjan Tokay, Rüdiger Köhling, Timo Kirschstein

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hippocampal synaptic plasticity between Schaffer collaterals and CA1 pyramidal neurons can be induced by activation of N-methyl-d-aspartate receptors (NMDARs) or of metabotropic glutamate receptors (mGluRs). Inhibitory GABAergic interneurons in this region abundantly terminate on pyramidal neurons and may thus influence synaptic plasticity. Although NMDAR-dependent synaptic plasticity is known to be influenced by inhibitory interneurons, little is known about the role of GABA on mGluR-dependent plasticity. Here, we used field potential recordings of the Schaffer collateral-CA1 synapses in rat hippocampal slices in order to study the effect of GABAA receptor (GABAAR) inhibition on mGluR-dependent long-term depression (LTD). Without GABAAR blockade, mGluR-dependent LTD was induced pharmacologically by the group I mGluR agonist (RS)-3,5-dihydroxyphenylglycine (DHPG, 100 μM, 10 min) as well as electrically by paired-pulse low-frequency stimulation (PP-LFS, 900 paired pulses at 1 Hz) resulting in a stable depression of the field response lasting at least 80 min after LTD induction. The GABAAR antagonist gabazine (5 μM) itself caused an increase of field responses suggesting an endogenous GABA release inhibiting CA1 field potentials. However, when either DHPG or PP-LFS was applied during GABAAR inhibition, the field responses were significantly reduced. Moreover, normalizing these responses to experiments without GABAAR blockade, there was no significant effect of gabazine on both DHPG- and PP-LFS-induced LTD. Thus, our results show that mGluR-dependent LTD at Schaffer collateral-CA1 synapses is unaffected by GABAAR mediated synaptic transmission.

Original languageEnglish
Pages (from-to)20-25
Number of pages6
JournalNeuroscience Letters
Volume467
Issue number1
DOIs
Publication statusPublished - Dec 18 2009
Externally publishedYes

Fingerprint

GABA-A Receptors
Synapses
Hippocampus
Neuronal Plasticity
Pyramidal Cells
Interneurons
gamma-Aminobutyric Acid
GABA-A Receptor Antagonists
Metabotropic Glutamate Receptors
Synaptic Transmission
aspartic acid receptor
gabazine
dihydroxyphenylethylene glycol
3,5-dihydroxyphenylglycine

Keywords

  • DHPG
  • Gabazine
  • Interneurons
  • LTD

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

GABAA receptor inhibition does not affect mGluR-dependent LTD at hippocampal Schaffer collateral-CA1 synapses. / Rohde, Marco; Tokay, Tursonjan; Köhling, Rüdiger; Kirschstein, Timo.

In: Neuroscience Letters, Vol. 467, No. 1, 18.12.2009, p. 20-25.

Research output: Contribution to journalArticle

Rohde, Marco ; Tokay, Tursonjan ; Köhling, Rüdiger ; Kirschstein, Timo. / GABAA receptor inhibition does not affect mGluR-dependent LTD at hippocampal Schaffer collateral-CA1 synapses. In: Neuroscience Letters. 2009 ; Vol. 467, No. 1. pp. 20-25.
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