Generation of SNP markers for short straw in oat (Avena sativa L.)

Pirjo Tanhuanpää, Ruslan Kalendar, Jaana Laurila, Alan H. Schulman, Outi Manninen, Elina Kiviharju

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Short straw is a desired trait in oat germplasm (Avena sativa L.). Marker-assisted selection, a key tool for achieving this objective, is limited by the presence and number of available markers. Here, we have attempted to develop markers sufficiently linked to a gene specifying short straw so that marker-assisted selection could be applied. Bulked-segregant analysis was used to identify anonymous PCR-based markers associated with the dwarfing gene Dw6 in an F2 population from the cross between A. sativa 'Aslak' and A. sativa 'Kontant'. One random amplified polymorphic DNA (RAPD) and 1 retrotransposon-microsatellite amplified polymorphism (REMAP) marker were found to be associated with height. These were converted into codominant single-nucleotide polymorphism (SNP) markers. The SNP-REMAP and the SNP-RAPD markers were located 5.2 and 12.6 cM from Dw6, respectively. They can be used in future efforts both to enhance oat germplasm by application of molecular markers and to determine the nature of the gene through positional cloning.

Original languageEnglish
Pages (from-to)282-287
Number of pages6
JournalGenome
Volume49
Issue number3
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

Keywords

  • Avena sativa
  • Marker-assisted selection
  • RAPD
  • REMAP
  • Short straw
  • SNP

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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