Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16

Alexandr Shevtsov, Erlan Ramanculov, Elena Shevtsova, Alma Kairzhanova, Pavel Tarlykov, Maxim Filipenko, Maya Dymova, Gulzada Abisheva, Aygul Jailbekova, Dinara Kamalova, Andrei Chsherbakov, Samat Tulegenov, Assel Akhmetova, Igor Sytnik, Talgat Karibaev, Kasim Mukanov

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Brucellosis is an endemic disease in Central Asia characterized by high infection rates in humans and animals. Currently, little is known about the genetic diversity of Brucella spp. circulating in the region, despite the high prevalence of brucellosis. This study aimed to analyze the genetic diversity of Brucella melitensis and Brucella abortus strains circulating in the Republic of Kazakhstan. We genotyped 128 B. melitensis and 124 B. abortus strains collected in regions with the highest prevalence of brucellosis. Genotyping was performed using multi-locus variable-number tandem-repeat analysis (MLVA). Analysis of a subset of 8 loci (MLVA-8) of 128 B. melitensis strains identified genotypes 42 (n=. 108), 43 (n=. 2), and 63 (n=. 19) related to the 'East Mediterranean' group. An MLVA-16 assay sorted 128 B. melitensis strains into 25 different genotypes. Excluding one variable locus, MLVA-15 of B. melitensis was distinct from strains originating in the Mediterranean region; however, 77% of them were identical to strains isolated in China. A minimum spanning tree for B. melitensis using MLVA-15 analysis clustered the local strains together with strains previously collected in China. MLVA-8 analysis of 124 B. abortus strains identified them as genotype 36, suggesting Eurasian distribution of this lineage. Complete MLVA-16 assay analysis clustered the strains into five genotypes, revealing little diversity of B. abortus when compared on the global scale. A minimum spanning tree for B. abortus obtained using MLVA-15 analysis clustered the 2 most prevalent genotypes (n=. 117) together with strains previously collected in China. Thus, MLVA analysis was used to characterize 252 strains of Brucella collected in Kazakhstan. The analysis revealed genetic homogeneity among the strains. Interestingly, identical MLVA-15 profiles were found in seemingly unrelated outbreaks in China, Turkey, and Kazakhstan. Further analysis is needed for better understanding of the epidemiology of brucellosis in Asia.

Original languageEnglish
Pages (from-to)173-180
Number of pages8
JournalInfection, Genetics and Evolution
Volume34
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

Kazakhstan
Brucella melitensis
Brucella abortus
Minisatellite Repeats
minisatellite repeats
Brucella melitensis biovar Abortus
genetic variation
loci
Brucellosis
Genotype
brucellosis
China
Brucella
genotype
genetic diversity
analysis
Central Asia
Mediterranean Region
Endemic Diseases
Turkey

Keywords

  • Brucella abortus
  • Brucella melitensis
  • Genotyping
  • Kazakhstan
  • Multi-locus variable number tandem repeat analysis (MLVA)

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16. / Shevtsov, Alexandr; Ramanculov, Erlan; Shevtsova, Elena; Kairzhanova, Alma; Tarlykov, Pavel; Filipenko, Maxim; Dymova, Maya; Abisheva, Gulzada; Jailbekova, Aygul; Kamalova, Dinara; Chsherbakov, Andrei; Tulegenov, Samat; Akhmetova, Assel; Sytnik, Igor; Karibaev, Talgat; Mukanov, Kasim.

In: Infection, Genetics and Evolution, Vol. 34, 01.08.2015, p. 173-180.

Research output: Contribution to journalArticle

Shevtsov, A, Ramanculov, E, Shevtsova, E, Kairzhanova, A, Tarlykov, P, Filipenko, M, Dymova, M, Abisheva, G, Jailbekova, A, Kamalova, D, Chsherbakov, A, Tulegenov, S, Akhmetova, A, Sytnik, I, Karibaev, T & Mukanov, K 2015, 'Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16', Infection, Genetics and Evolution, vol. 34, pp. 173-180. https://doi.org/10.1016/j.meegid.2015.07.008
Shevtsov, Alexandr ; Ramanculov, Erlan ; Shevtsova, Elena ; Kairzhanova, Alma ; Tarlykov, Pavel ; Filipenko, Maxim ; Dymova, Maya ; Abisheva, Gulzada ; Jailbekova, Aygul ; Kamalova, Dinara ; Chsherbakov, Andrei ; Tulegenov, Samat ; Akhmetova, Assel ; Sytnik, Igor ; Karibaev, Talgat ; Mukanov, Kasim. / Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16. In: Infection, Genetics and Evolution. 2015 ; Vol. 34. pp. 173-180.
@article{af17a5b00d454fd0b68216d1bb00874c,
title = "Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16",
abstract = "Brucellosis is an endemic disease in Central Asia characterized by high infection rates in humans and animals. Currently, little is known about the genetic diversity of Brucella spp. circulating in the region, despite the high prevalence of brucellosis. This study aimed to analyze the genetic diversity of Brucella melitensis and Brucella abortus strains circulating in the Republic of Kazakhstan. We genotyped 128 B. melitensis and 124 B. abortus strains collected in regions with the highest prevalence of brucellosis. Genotyping was performed using multi-locus variable-number tandem-repeat analysis (MLVA). Analysis of a subset of 8 loci (MLVA-8) of 128 B. melitensis strains identified genotypes 42 (n=. 108), 43 (n=. 2), and 63 (n=. 19) related to the 'East Mediterranean' group. An MLVA-16 assay sorted 128 B. melitensis strains into 25 different genotypes. Excluding one variable locus, MLVA-15 of B. melitensis was distinct from strains originating in the Mediterranean region; however, 77{\%} of them were identical to strains isolated in China. A minimum spanning tree for B. melitensis using MLVA-15 analysis clustered the local strains together with strains previously collected in China. MLVA-8 analysis of 124 B. abortus strains identified them as genotype 36, suggesting Eurasian distribution of this lineage. Complete MLVA-16 assay analysis clustered the strains into five genotypes, revealing little diversity of B. abortus when compared on the global scale. A minimum spanning tree for B. abortus obtained using MLVA-15 analysis clustered the 2 most prevalent genotypes (n=. 117) together with strains previously collected in China. Thus, MLVA analysis was used to characterize 252 strains of Brucella collected in Kazakhstan. The analysis revealed genetic homogeneity among the strains. Interestingly, identical MLVA-15 profiles were found in seemingly unrelated outbreaks in China, Turkey, and Kazakhstan. Further analysis is needed for better understanding of the epidemiology of brucellosis in Asia.",
keywords = "Brucella abortus, Brucella melitensis, Genotyping, Kazakhstan, Multi-locus variable number tandem repeat analysis (MLVA)",
author = "Alexandr Shevtsov and Erlan Ramanculov and Elena Shevtsova and Alma Kairzhanova and Pavel Tarlykov and Maxim Filipenko and Maya Dymova and Gulzada Abisheva and Aygul Jailbekova and Dinara Kamalova and Andrei Chsherbakov and Samat Tulegenov and Assel Akhmetova and Igor Sytnik and Talgat Karibaev and Kasim Mukanov",
year = "2015",
month = "8",
day = "1",
doi = "10.1016/j.meegid.2015.07.008",
language = "English",
volume = "34",
pages = "173--180",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",

}

TY - JOUR

T1 - Genetic diversity of Brucella abortus and Brucella melitensis in Kazakhstan using MLVA-16

AU - Shevtsov, Alexandr

AU - Ramanculov, Erlan

AU - Shevtsova, Elena

AU - Kairzhanova, Alma

AU - Tarlykov, Pavel

AU - Filipenko, Maxim

AU - Dymova, Maya

AU - Abisheva, Gulzada

AU - Jailbekova, Aygul

AU - Kamalova, Dinara

AU - Chsherbakov, Andrei

AU - Tulegenov, Samat

AU - Akhmetova, Assel

AU - Sytnik, Igor

AU - Karibaev, Talgat

AU - Mukanov, Kasim

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Brucellosis is an endemic disease in Central Asia characterized by high infection rates in humans and animals. Currently, little is known about the genetic diversity of Brucella spp. circulating in the region, despite the high prevalence of brucellosis. This study aimed to analyze the genetic diversity of Brucella melitensis and Brucella abortus strains circulating in the Republic of Kazakhstan. We genotyped 128 B. melitensis and 124 B. abortus strains collected in regions with the highest prevalence of brucellosis. Genotyping was performed using multi-locus variable-number tandem-repeat analysis (MLVA). Analysis of a subset of 8 loci (MLVA-8) of 128 B. melitensis strains identified genotypes 42 (n=. 108), 43 (n=. 2), and 63 (n=. 19) related to the 'East Mediterranean' group. An MLVA-16 assay sorted 128 B. melitensis strains into 25 different genotypes. Excluding one variable locus, MLVA-15 of B. melitensis was distinct from strains originating in the Mediterranean region; however, 77% of them were identical to strains isolated in China. A minimum spanning tree for B. melitensis using MLVA-15 analysis clustered the local strains together with strains previously collected in China. MLVA-8 analysis of 124 B. abortus strains identified them as genotype 36, suggesting Eurasian distribution of this lineage. Complete MLVA-16 assay analysis clustered the strains into five genotypes, revealing little diversity of B. abortus when compared on the global scale. A minimum spanning tree for B. abortus obtained using MLVA-15 analysis clustered the 2 most prevalent genotypes (n=. 117) together with strains previously collected in China. Thus, MLVA analysis was used to characterize 252 strains of Brucella collected in Kazakhstan. The analysis revealed genetic homogeneity among the strains. Interestingly, identical MLVA-15 profiles were found in seemingly unrelated outbreaks in China, Turkey, and Kazakhstan. Further analysis is needed for better understanding of the epidemiology of brucellosis in Asia.

AB - Brucellosis is an endemic disease in Central Asia characterized by high infection rates in humans and animals. Currently, little is known about the genetic diversity of Brucella spp. circulating in the region, despite the high prevalence of brucellosis. This study aimed to analyze the genetic diversity of Brucella melitensis and Brucella abortus strains circulating in the Republic of Kazakhstan. We genotyped 128 B. melitensis and 124 B. abortus strains collected in regions with the highest prevalence of brucellosis. Genotyping was performed using multi-locus variable-number tandem-repeat analysis (MLVA). Analysis of a subset of 8 loci (MLVA-8) of 128 B. melitensis strains identified genotypes 42 (n=. 108), 43 (n=. 2), and 63 (n=. 19) related to the 'East Mediterranean' group. An MLVA-16 assay sorted 128 B. melitensis strains into 25 different genotypes. Excluding one variable locus, MLVA-15 of B. melitensis was distinct from strains originating in the Mediterranean region; however, 77% of them were identical to strains isolated in China. A minimum spanning tree for B. melitensis using MLVA-15 analysis clustered the local strains together with strains previously collected in China. MLVA-8 analysis of 124 B. abortus strains identified them as genotype 36, suggesting Eurasian distribution of this lineage. Complete MLVA-16 assay analysis clustered the strains into five genotypes, revealing little diversity of B. abortus when compared on the global scale. A minimum spanning tree for B. abortus obtained using MLVA-15 analysis clustered the 2 most prevalent genotypes (n=. 117) together with strains previously collected in China. Thus, MLVA analysis was used to characterize 252 strains of Brucella collected in Kazakhstan. The analysis revealed genetic homogeneity among the strains. Interestingly, identical MLVA-15 profiles were found in seemingly unrelated outbreaks in China, Turkey, and Kazakhstan. Further analysis is needed for better understanding of the epidemiology of brucellosis in Asia.

KW - Brucella abortus

KW - Brucella melitensis

KW - Genotyping

KW - Kazakhstan

KW - Multi-locus variable number tandem repeat analysis (MLVA)

UR - http://www.scopus.com/inward/record.url?scp=84937954850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937954850&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2015.07.008

DO - 10.1016/j.meegid.2015.07.008

M3 - Article

C2 - 26160544

AN - SCOPUS:84937954850

VL - 34

SP - 173

EP - 180

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

ER -