TY - JOUR
T1 - Genomic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Strains From Patients in Kazakhstan
AU - Daniyarov, Asset
AU - Molkenov, Askhat
AU - Rakhimova, Saule
AU - Akhmetova, Ainur
AU - Yerezhepov, Dauren
AU - Chingissova, Lyailya
AU - Bismilda, Venera
AU - Toksanbayeva, Bekzat
AU - Rakisheva, Anar
AU - Akilzhanova, Ainur
AU - Kozhamkulov, Ulan
AU - Kairov, Ulykbek
N1 - Funding Information:
This work was supported by the research grant of the Ministry of Education and Science of the Republic of Kazakhstan (AP09259750, Prevalence, drug resistance and genotypic diversity of M.tuberculosis Beijing family in Kazakhstan).
Publisher Copyright:
Copyright © 2021 Daniyarov, Molkenov, Rakhimova, Akhmetova, Yerezhepov, Chingissova, Bismilda, Toksanbayeva, Rakisheva, Akilzhanova, Kozhamkulov and Kairov.
PY - 2021/11/9
Y1 - 2021/11/9
N2 - Tuberculosis (TB) is an infectious disease that remains an essential public health problem in many countries. Despite decreasing numbers of new cases worldwide, the incidence of antibiotic-resistant forms (multidrug resistant and extensively drug-resistant) of TB is increasing. Next-generation sequencing technologies provide a high-throughput approach to identify known and novel potential genetic variants that are associated with drug resistance in Mycobacterium tuberculosis (Mtb). There are limited reports and data related to whole-genome characteristics of drug-resistant Mtb strains circulating in Kazakhstan. Here, we report whole-genome sequencing and analysis results of eight multidrug-resistant strains collected from TB patients in Kazakhstan. Genotyping and validation of all strains by MIRU-VNTR and spoligotyping methodologies revealed that these strains belong to the Beijing family. The spectrum of specific and potentially novel genomic variants (single-nucleotide polymorphisms, insertions, and deletions) related to drug resistance was identified and annotated. ResFinder, CARD, and CASTB antibiotic resistance databases were used for the characterization of genetic variants in genes associated with drug resistance. Our results provide reference data and genomic profiles of multidrug-resistant isolates for further comparative studies and investigations of genetic patterns in drug-resistant Mtb strains.
AB - Tuberculosis (TB) is an infectious disease that remains an essential public health problem in many countries. Despite decreasing numbers of new cases worldwide, the incidence of antibiotic-resistant forms (multidrug resistant and extensively drug-resistant) of TB is increasing. Next-generation sequencing technologies provide a high-throughput approach to identify known and novel potential genetic variants that are associated with drug resistance in Mycobacterium tuberculosis (Mtb). There are limited reports and data related to whole-genome characteristics of drug-resistant Mtb strains circulating in Kazakhstan. Here, we report whole-genome sequencing and analysis results of eight multidrug-resistant strains collected from TB patients in Kazakhstan. Genotyping and validation of all strains by MIRU-VNTR and spoligotyping methodologies revealed that these strains belong to the Beijing family. The spectrum of specific and potentially novel genomic variants (single-nucleotide polymorphisms, insertions, and deletions) related to drug resistance was identified and annotated. ResFinder, CARD, and CASTB antibiotic resistance databases were used for the characterization of genetic variants in genes associated with drug resistance. Our results provide reference data and genomic profiles of multidrug-resistant isolates for further comparative studies and investigations of genetic patterns in drug-resistant Mtb strains.
KW - genetic variants
KW - genomic analysis
KW - MIRU-VNTR analysis
KW - multidrug resistant TB
KW - Mycobacterium tuberculosis
KW - spoligotyping
KW - tuberculosis
KW - whole genome
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UR - http://www.scopus.com/inward/citedby.url?scp=85120673760&partnerID=8YFLogxK
U2 - 10.3389/fgene.2021.683515
DO - 10.3389/fgene.2021.683515
M3 - Article
AN - SCOPUS:85120673760
SN - 1664-8021
VL - 12
JO - Frontiers in Genetics
JF - Frontiers in Genetics
M1 - 683515
ER -
