Genotyping, morphology and molecular characteristics of a lytic phage of Neisseria strain obtained from infected human dental plaque

Ahmed N. Aljarbou, Mohamad Aljofan

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The lytic bacteriaphage (phage) A2 was isolated from human dental plaques along with its bacterial host. The virus was found to have an icosahedron-shaped head (60±3 nm), a sheathed and rigid long tail (~175 nm) and was categorized into the family Siphoviridae of the order Caudovirales, which are dsDNA viral family, characterised by their ability to infect bacteria and are nonenveloped with a noncontractile tail. The isolated phage contained a linear dsDNA genome having 31,703 base pairs of unique sequence, which were sorted into three contigs and 12 single sequences. A latent period of 25 minutes and burst size of 24±2 particles was determined for the virus. Bioinformatics approaches were used to identify ORFs in the genome. A phylogenetic analysis confirmed the species inter-relationship and its placement in the family.

Original languageEnglish
Pages (from-to)609-618
Number of pages10
JournalJournal of Microbiology
Volume52
Issue number7
DOIs
Publication statusPublished - Jun 2014
Externally publishedYes

Keywords

  • contigs
  • human dental plaques
  • lytic phage A2
  • Neisseria sp.
  • phylogenetic relationships
  • Siphoviridae

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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