Gut microbiome diversity in kazakhstani women of different age groups

Dinara Baiskhanova, Almagul Kushugulova, Samat Kozhakhmetov, Zhanagul Khassenbekova, Talgat Nurgozhin, Francesco Marotta, Zhaxybay Zhumadilov

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Abstract

Human gut microflora includes three enterotypes and composes the largest part of the whole microbiome. Because gut microflora play a crucial role in health and disease, knowing the microbial composition of the gut microbiome is an important task. Currently, the gut microbiome composition in the Kazakhstani population is poorly understood. Therefore, we studied the phylogenetic composition and types of the gut microbiome in Kazakhstani women and stratified the data by age. Female subjects were divided into three groups: six young (<44 years old), eighteen middle-aged (50–70 years old), and six elderly (>90 years old). For the gut microbiome composition analysis, the 16S rDNA locus was amplified, ligated into high copy plasmid vectors, and sequenced. We observed that two gut microbiome types, Phyla Bacteroidetes and Firmicutes, were age independent. However, there were significant differences in species composition between groups that can be attributed to age. The highest species diversity occurred in the middle-aged group, which subsequently decreased in the elderly group.
Original languageEnglish
Pages (from-to)97-108
Number of pages12
JournalInternational Journal of Probiotics and Prebiotics
Publication statusPublished - 2015

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digestive system
Age Groups
intestinal microorganisms
middle-aged adults
species diversity
plasmid vectors
Bacteroidetes
Firmicutes
Microbiota
Ribosomal DNA
microbiome
Gastrointestinal Microbiome
Plasmids
loci
phylogeny
Health
Population

Keywords

  • Age, Compositional structure, Diversity, Gut, Microbiome, Species

Cite this

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abstract = "Human gut microflora includes three enterotypes and composes the largest part of the whole microbiome. Because gut microflora play a crucial role in health and disease, knowing the microbial composition of the gut microbiome is an important task. Currently, the gut microbiome composition in the Kazakhstani population is poorly understood. Therefore, we studied the phylogenetic composition and types of the gut microbiome in Kazakhstani women and stratified the data by age. Female subjects were divided into three groups: six young (<44 years old), eighteen middle-aged (50–70 years old), and six elderly (>90 years old). For the gut microbiome composition analysis, the 16S rDNA locus was amplified, ligated into high copy plasmid vectors, and sequenced. We observed that two gut microbiome types, Phyla Bacteroidetes and Firmicutes, were age independent. However, there were significant differences in species composition between groups that can be attributed to age. The highest species diversity occurred in the middle-aged group, which subsequently decreased in the elderly group.",
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T1 - Gut microbiome diversity in kazakhstani women of different age groups

AU - Baiskhanova, Dinara

AU - Kushugulova, Almagul

AU - Kozhakhmetov, Samat

AU - Khassenbekova, Zhanagul

AU - Nurgozhin, Talgat

AU - Marotta, Francesco

AU - Zhumadilov, Zhaxybay

PY - 2015

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AB - Human gut microflora includes three enterotypes and composes the largest part of the whole microbiome. Because gut microflora play a crucial role in health and disease, knowing the microbial composition of the gut microbiome is an important task. Currently, the gut microbiome composition in the Kazakhstani population is poorly understood. Therefore, we studied the phylogenetic composition and types of the gut microbiome in Kazakhstani women and stratified the data by age. Female subjects were divided into three groups: six young (<44 years old), eighteen middle-aged (50–70 years old), and six elderly (>90 years old). For the gut microbiome composition analysis, the 16S rDNA locus was amplified, ligated into high copy plasmid vectors, and sequenced. We observed that two gut microbiome types, Phyla Bacteroidetes and Firmicutes, were age independent. However, there were significant differences in species composition between groups that can be attributed to age. The highest species diversity occurred in the middle-aged group, which subsequently decreased in the elderly group.

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