TY - JOUR
T1 - Gut modulation of dysbiosis induced by dextran sulfate sodium
AU - Kozhakhmetov, Samat
AU - Babenko, Dmitriy
AU - Kozhakhmetova, Saniya
AU - Tuyakova, Altynay
AU - Nurgaziyev, Madiyar
AU - Nurgozhina, Ayaulym
AU - Muhanbetganov, Nurislam
AU - Chulenbayeva, Laura
AU - Sergazy, Shynggys
AU - Gulyayev, Alexander
AU - Tanabayev, Baimakhan
AU - Saliev, Timur
AU - Kushugulova, Almagul
N1 - Publisher Copyright:
© 2021 The Author(s)
PY - 2021/8
Y1 - 2021/8
N2 - Inflammatory bowel disease is one of the serious burdens of clinical medicine and healthcare. This study investigated the potential of a biological product based on mare's milk and metabolites of symbiotic microflora for modulation of intestinal microflora affected by dextran sulfate sodium (DSS)-induced dysbiosis. Symbiotic microflora was isolated from the stool of healthy volunteers. Lysates for the production of short-chain fatty acids of screened microorganisms were mixed with mare's milk. The activity of the biological product was evaluated on the DSS model of induced colitis. Histological changes in the intestinal epithelium were determined. The structure of the microbiome was evaluated based on the analysis of 16S rRNA microbial sequences. Histological examination of rat intestinal tissues after application of the biological product showed reduced infiltration of granulocytes, macrophages, and lymphocytes. The results of sequencing demonstrated a decrease in the biological diversity of microbiota affected by colitis. The full recovery was observed after 21 days of the application of the biological product. The product induced the structural changes of the microbiome damaged by DSS. Likewise, the number of the pathogenic intestinal microflora was decreased Representatives of SCFA producing bacteria increased concentrations of genus Lactobacillus.
AB - Inflammatory bowel disease is one of the serious burdens of clinical medicine and healthcare. This study investigated the potential of a biological product based on mare's milk and metabolites of symbiotic microflora for modulation of intestinal microflora affected by dextran sulfate sodium (DSS)-induced dysbiosis. Symbiotic microflora was isolated from the stool of healthy volunteers. Lysates for the production of short-chain fatty acids of screened microorganisms were mixed with mare's milk. The activity of the biological product was evaluated on the DSS model of induced colitis. Histological changes in the intestinal epithelium were determined. The structure of the microbiome was evaluated based on the analysis of 16S rRNA microbial sequences. Histological examination of rat intestinal tissues after application of the biological product showed reduced infiltration of granulocytes, macrophages, and lymphocytes. The results of sequencing demonstrated a decrease in the biological diversity of microbiota affected by colitis. The full recovery was observed after 21 days of the application of the biological product. The product induced the structural changes of the microbiome damaged by DSS. Likewise, the number of the pathogenic intestinal microflora was decreased Representatives of SCFA producing bacteria increased concentrations of genus Lactobacillus.
KW - Biological product
KW - DSS-Induced colitis
KW - Gut microbiome
KW - Microbial diversity
KW - Rats
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U2 - 10.1016/j.fbio.2021.101167
DO - 10.1016/j.fbio.2021.101167
M3 - Article
AN - SCOPUS:85107728736
SN - 2212-4292
VL - 42
JO - Food Bioscience
JF - Food Bioscience
M1 - 101167
ER -