H3K9 and H4K20 methyltransferases are directly involved in the heterochromatinization of the paternal chromosomes in male Planococcus citri embryos

Yakov A. Osipov; Olga V. Posukh; Darya A. Kalashnikova; Polina A. Antoshina; Petr P. Laktionov; Polina A. Skrypnik; Stepan N. Belyakin; Prim B. Singh

doi:10.1007/s00412-023-00809-3

H3K9 and H4K20 methyltransferases are directly involved in the heterochromatinization of the paternal chromosomes in male Planococcus citri embryos

Yakov A. Osipov, Olga V. Posukh, Darya A. Kalashnikova, Polina A. Antoshina, Petr P. Laktionov, Polina A. Skrypnik, Stepan N. Belyakin, Prim B. Singh

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Using a new method for bulk preparation of early stage embryos, we have investigated the role played by putative Planococcus citri H3K9 and H4K20 histone methyl transferases (HMTases) in regulating heterochromatinization of the imprinted paternal chromosomal set in male embryos. We found that H3K9 and H420 HMTases are required for heterochromatinization of the paternal chromosomes. We present evidence that both HMTases maintain the paternal “imprint” during the cleavage divisions when both parental chromosome sets are euchromatic. A testable model that accommodates our findings is proposed.

Original language	English
Pages (from-to)	317-328
Number of pages	12
Journal	Chromosoma
Volume	132
Issue number	4
DOIs	https://doi.org/10.1007/s00412-023-00809-3
Publication status	Published - Nov 2023
Externally published	Yes

Funding

This work was funded by a grant from the Ministry of Education and Science of Russian Federation #14.Y26.31.0024 and Nazarbayev University Faculty Development Grant 021220FD2451 awarded to PBS. It was partially supported by the Russian Fundamental Scientific Research Project: FWGZ-2021–0017.

Funders	Funder number
Ministry for Education and Science of the Russian Federation
Nazarbayev University	021220FD2451, FWGZ-2021–0017

Keywords

Epigenetics
Genomic imprinting
H3K9me3:HP1:H4K20me3 pathway
Heterochromatin

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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10.1007/s00412-023-00809-3

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title = "H3K9 and H4K20 methyltransferases are directly involved in the heterochromatinization of the paternal chromosomes in male Planococcus citri embryos",

abstract = "Using a new method for bulk preparation of early stage embryos, we have investigated the role played by putative Planococcus citri H3K9 and H4K20 histone methyl transferases (HMTases) in regulating heterochromatinization of the imprinted paternal chromosomal set in male embryos. We found that H3K9 and H420 HMTases are required for heterochromatinization of the paternal chromosomes. We present evidence that both HMTases maintain the paternal “imprint” during the cleavage divisions when both parental chromosome sets are euchromatic. A testable model that accommodates our findings is proposed.",

keywords = "Epigenetics, Genomic imprinting, H3K9me3:HP1:H4K20me3 pathway, Heterochromatin",

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note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2023",

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doi = "10.1007/s00412-023-00809-3",

language = "English",

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T1 - H3K9 and H4K20 methyltransferases are directly involved in the heterochromatinization of the paternal chromosomes in male Planococcus citri embryos

AU - Osipov, Yakov A.

AU - Posukh, Olga V.

AU - Kalashnikova, Darya A.

AU - Antoshina, Polina A.

AU - Laktionov, Petr P.

AU - Skrypnik, Polina A.

AU - Belyakin, Stepan N.

AU - Singh, Prim B.

PY - 2023/11

Y1 - 2023/11

N2 - Using a new method for bulk preparation of early stage embryos, we have investigated the role played by putative Planococcus citri H3K9 and H4K20 histone methyl transferases (HMTases) in regulating heterochromatinization of the imprinted paternal chromosomal set in male embryos. We found that H3K9 and H420 HMTases are required for heterochromatinization of the paternal chromosomes. We present evidence that both HMTases maintain the paternal “imprint” during the cleavage divisions when both parental chromosome sets are euchromatic. A testable model that accommodates our findings is proposed.

AB - Using a new method for bulk preparation of early stage embryos, we have investigated the role played by putative Planococcus citri H3K9 and H4K20 histone methyl transferases (HMTases) in regulating heterochromatinization of the imprinted paternal chromosomal set in male embryos. We found that H3K9 and H420 HMTases are required for heterochromatinization of the paternal chromosomes. We present evidence that both HMTases maintain the paternal “imprint” during the cleavage divisions when both parental chromosome sets are euchromatic. A testable model that accommodates our findings is proposed.

KW - Epigenetics

KW - Genomic imprinting

KW - H3K9me3:HP1:H4K20me3 pathway

KW - Heterochromatin

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H3K9 and H4K20 methyltransferases are directly involved in the heterochromatinization of the paternal chromosomes in male Planococcus citri embryos

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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