HEAT repeats mediate plasma membrane localization of Tor2p in yeast

Jeannette Kunz, Ulrich Schneider, Isabelle Howald, Anja Schmidt, Michael N. Hall

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

The subcellular distribution of Tor1p and Tor2p, two phosphatidylinositol kinase homologs and targets of the immunosuppressive drug rapamycin in Saccharomyces cerevisiae, was analyzed. We found that Tor protein is peripherally associated with membranes. Subcellular fractionation and immunofluorescence studies showed that Tor1p and Tor2p associate with the plasma membrane and a second fraction that is distinct from Golgi, vacuoles, mitochondria, and nucleus and may represent vesicular structures. Pulse-chase experiments showed that association of Tot protein with plasma membrane and the second compartment is fast, does not appear to involve components of endocytic, secretory, or Golgi to vacuole transport pathways, and is not affected by the immunosuppressive drug rapamycin. Deletion analysis reveals that two domains within Tor2p independently mediate localization to both compartments. These domains are composed of HEAT repeats that are thought to act as protein-protein interaction surfaces. Our studies therefore place Tor proteins at the site of action of their known downstream effectors and suggest that they may be part of a multiprotein complex.

Original languageEnglish
Pages (from-to)37011-37020
Number of pages10
JournalJournal of Biological Chemistry
Volume275
Issue number47
DOIs
Publication statusPublished - Nov 24 2000
Externally publishedYes

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Cell membranes
Yeast
Yeasts
Cell Membrane
Sirolimus
Immunosuppressive Agents
Vacuoles
Proteins
Secretory Component
Multiprotein Complexes
Phosphatidylinositols
Mitochondria
Pharmaceutical Preparations
Fluorescent Antibody Technique
Saccharomyces cerevisiae
Fractionation
Membrane Proteins
Phosphotransferases
Membranes
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

HEAT repeats mediate plasma membrane localization of Tor2p in yeast. / Kunz, Jeannette; Schneider, Ulrich; Howald, Isabelle; Schmidt, Anja; Hall, Michael N.

In: Journal of Biological Chemistry, Vol. 275, No. 47, 24.11.2000, p. 37011-37020.

Research output: Contribution to journalArticle

Kunz, J, Schneider, U, Howald, I, Schmidt, A & Hall, MN 2000, 'HEAT repeats mediate plasma membrane localization of Tor2p in yeast', Journal of Biological Chemistry, vol. 275, no. 47, pp. 37011-37020. https://doi.org/10.1074/jbc.M007296200
Kunz, Jeannette ; Schneider, Ulrich ; Howald, Isabelle ; Schmidt, Anja ; Hall, Michael N. / HEAT repeats mediate plasma membrane localization of Tor2p in yeast. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 47. pp. 37011-37020.
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