TY - JOUR
T1 - Heat shock mediated labelling of Pseudomonas aeruginosa with quantum dots
AU - Kumar, Natasha
AU - Wiraja, Christian
AU - Palanisamy, Kannan
AU - Marsili, Enrico
AU - Xu, Chenjie
N1 - Funding Information:
This research was supported by NTU-Northwestern Institute for Nanomedicine. We thank Dr. Sharon Longford, Senior Assistant Director, Science Communications at SCELSE for her help with the editing of the manuscript.
Publisher Copyright:
© 2016 Elsevier B.V.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Biocompatible nanoparticles are good candidates to label bacteria for imaging and diagnosis purposes. A high labeling efficiency reduces the concentration of nanoparticles required for labeling and allows the labeled bacteria to be tracked for longer periods. This report explores the optimal labeling strategy for Pseudomonas aeruginosa, a common gram-negative opportunistic pathogen, with quantum dots. Three strategies including direct incubation, calcium chloride treatment, and heat shock are compared and the labeling efficiency is assessed through fluorescence microscopy and flow cytometry analysis. Of the three, heat shock is finally selected due to its comparable labeling efficiency and simplicity. Through the assay of the respiration rate of bacteria together with morphology analysis, the heat shock process does not show any negative effect over the cells activity even at sub-toxic concentrations.
AB - Biocompatible nanoparticles are good candidates to label bacteria for imaging and diagnosis purposes. A high labeling efficiency reduces the concentration of nanoparticles required for labeling and allows the labeled bacteria to be tracked for longer periods. This report explores the optimal labeling strategy for Pseudomonas aeruginosa, a common gram-negative opportunistic pathogen, with quantum dots. Three strategies including direct incubation, calcium chloride treatment, and heat shock are compared and the labeling efficiency is assessed through fluorescence microscopy and flow cytometry analysis. Of the three, heat shock is finally selected due to its comparable labeling efficiency and simplicity. Through the assay of the respiration rate of bacteria together with morphology analysis, the heat shock process does not show any negative effect over the cells activity even at sub-toxic concentrations.
KW - Bacteria labeling
KW - Heat shock
KW - Pseudomonas aeruginosa
KW - Quantum dots
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U2 - 10.1016/j.colsurfb.2016.02.052
DO - 10.1016/j.colsurfb.2016.02.052
M3 - Article
C2 - 26962762
AN - SCOPUS:84959560353
VL - 142
SP - 259
EP - 265
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -