TY - JOUR
T1 - HER2 gene amplification in breast cancer - A rogues' gallery of challenging diagnostic cases
T2 - UKNEQAS interpretation guidelines and research recommendations
AU - Starczynski, Jane
AU - Atkey, Neil
AU - Connelly, Yvonne
AU - O'Grady, Tony
AU - Campbell, Fiona M.
AU - Di Palma, Silvana
AU - Wencyk, Peter
AU - Jasani, Bharat
AU - Gandy, Michael
AU - Bartlett, J. M.S.
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/4
Y1 - 2012/4
N2 - International and national guidelines highlight the importance of accuracy, reproducibility, and quality control of in situ hybridization (ISH) methods for testing breast carcinomas. However, few guidelines cover the reporting of ISH cases with "unusual" signal patterns, including, eg, heterogeneity and loss of chromosome enumeration probe or gene signals. These cases are, in fact, relatively frequent, and there is a need for developing evidence- or consensusbased reporting guidelines to ensure consistency of treatment. Following an audit of cases from a single center (including >1,700 cases) we show that approximately 10% of ISH results reflect unusual signal patterns. We illustrate the most common of these patterns and provide reporting guidelines for diagnosticians and recommendations for future research. Our goal is to ensure that in the future such "rogues" are reported in a consistent manner that, ultimately, will be supported by molecular and biochemical evidence. Copyright
AB - International and national guidelines highlight the importance of accuracy, reproducibility, and quality control of in situ hybridization (ISH) methods for testing breast carcinomas. However, few guidelines cover the reporting of ISH cases with "unusual" signal patterns, including, eg, heterogeneity and loss of chromosome enumeration probe or gene signals. These cases are, in fact, relatively frequent, and there is a need for developing evidence- or consensusbased reporting guidelines to ensure consistency of treatment. Following an audit of cases from a single center (including >1,700 cases) we show that approximately 10% of ISH results reflect unusual signal patterns. We illustrate the most common of these patterns and provide reporting guidelines for diagnosticians and recommendations for future research. Our goal is to ensure that in the future such "rogues" are reported in a consistent manner that, ultimately, will be supported by molecular and biochemical evidence. Copyright
KW - Breast cancer
KW - Evidence-based reporting
KW - Guidelines
KW - HER2 gene amplification
KW - In situ hybridization
KW - Research
UR - http://www.scopus.com/inward/record.url?scp=84860595433&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860595433&partnerID=8YFLogxK
U2 - 10.1309/AJCPATBZ2JFN1QQC
DO - 10.1309/AJCPATBZ2JFN1QQC
M3 - Article
C2 - 22431536
AN - SCOPUS:84860595433
VL - 137
SP - 595
EP - 605
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 4
ER -