HER2 gene amplification in breast cancer - A rogues' gallery of challenging diagnostic cases: UKNEQAS interpretation guidelines and research recommendations

Jane Starczynski, Neil Atkey, Yvonne Connelly, Tony O'Grady, Fiona M. Campbell, Silvana Di Palma, Peter Wencyk, Bharat Jasani, Michael Gandy, J. M.S. Bartlett

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57 Citations (Scopus)

Abstract

International and national guidelines highlight the importance of accuracy, reproducibility, and quality control of in situ hybridization (ISH) methods for testing breast carcinomas. However, few guidelines cover the reporting of ISH cases with "unusual" signal patterns, including, eg, heterogeneity and loss of chromosome enumeration probe or gene signals. These cases are, in fact, relatively frequent, and there is a need for developing evidence- or consensusbased reporting guidelines to ensure consistency of treatment. Following an audit of cases from a single center (including >1,700 cases) we show that approximately 10% of ISH results reflect unusual signal patterns. We illustrate the most common of these patterns and provide reporting guidelines for diagnosticians and recommendations for future research. Our goal is to ensure that in the future such "rogues" are reported in a consistent manner that, ultimately, will be supported by molecular and biochemical evidence. Copyright

Original languageEnglish
Pages (from-to)595-605
Number of pages11
JournalAmerican Journal of Clinical Pathology
Volume137
Issue number4
DOIs
Publication statusPublished - Apr 1 2012

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Keywords

  • Breast cancer
  • Evidence-based reporting
  • Guidelines
  • HER2 gene amplification
  • In situ hybridization
  • Research

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Starczynski, J., Atkey, N., Connelly, Y., O'Grady, T., Campbell, F. M., Di Palma, S., Wencyk, P., Jasani, B., Gandy, M., & Bartlett, J. M. S. (2012). HER2 gene amplification in breast cancer - A rogues' gallery of challenging diagnostic cases: UKNEQAS interpretation guidelines and research recommendations. American Journal of Clinical Pathology, 137(4), 595-605. https://doi.org/10.1309/AJCPATBZ2JFN1QQC