Heterochromatin and tri-methylated lysine 20 of histone H4 in animals

Niki Kourmouli, Peter Jeppesen, Shantha Mahadevhaiah, Paul Burgoyne, Rong Wu, David M Gilbert, Silvia Bongiorni, Giorgio Prantera, Laura Fanti, Sergio Pimpinelli, Wei Shi, Reinald Fundele, Prim B Singh

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Tri-methylated lysine 20 on histone H4 (Me(3)K20H4) is a marker of constitutive heterochromatin in murine interphase and metaphase cells. Heterochromatin marked by Me(3)K20H4 replicates late during S phase of the cell cycle. Serum starvation increases the number of cells that exhibit high levels of Me(3)K20H4 at constitutive heterochromatin. Me(3)K20H4 is also present at the centromeric heterochromatin of most meiotic chromosomes during spermatogenesis and at the pseudoautosomal region, as well as at some telomeres. It is not present on the XY-body. During murine embryogenesis the maternal pronucleus contains Me(3)K20H4; Me(3)K20H4 is absent from the paternal pronucleus. On Drosophila polytene chromosomes Me(3)K20H4 is present in a 'punctate pattern' at many chromosomal bands, including the chromocenter. In coccids it is present on the facultatively heterochromatinised paternal chromosome set. We also present evidence that Me(3)K20H4 is dependent upon H3-specific Suv(3)9 histone methyltransferase activity, suggesting that there may be 'epigenetic cross-talk' between histones H3 and H4.

Original languageEnglish
Pages (from-to)2491-501
Number of pages11
JournalJournal of Cell Science
Volume117
Issue numberPt 12
DOIs
Publication statusPublished - May 15 2004

Fingerprint

Heterochromatin
Histones
Lysine
Chromosomes
Polytene Chromosomes
Chromosomes, Human, Pair 3
Interphase
Telomere
Spermatogenesis
Metaphase
Starvation
S Phase
Epigenomics
Drosophila
Embryonic Development
Cell Cycle
Cell Count
Mothers
Serum

Keywords

  • Animals
  • Cell Line
  • Cell Nucleus
  • Cells, Cultured
  • Centromere
  • Chromosomes
  • Culture Media, Serum-Free
  • DNA
  • Drosophila
  • Embryo, Mammalian
  • Embryo, Nonmammalian
  • Female
  • Fibroblasts
  • Fluorescent Antibody Technique, Indirect
  • Genetic Markers
  • Hemiptera
  • Heterochromatin
  • Histones
  • Lysine
  • Male
  • Methylation
  • Mice
  • Microscopy, Fluorescence
  • Oocytes
  • S Phase
  • Spermatozoa
  • Telomere
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Kourmouli, N., Jeppesen, P., Mahadevhaiah, S., Burgoyne, P., Wu, R., Gilbert, D. M., ... Singh, P. B. (2004). Heterochromatin and tri-methylated lysine 20 of histone H4 in animals. Journal of Cell Science, 117(Pt 12), 2491-501. https://doi.org/10.1242/jcs.01238

Heterochromatin and tri-methylated lysine 20 of histone H4 in animals. / Kourmouli, Niki; Jeppesen, Peter; Mahadevhaiah, Shantha; Burgoyne, Paul; Wu, Rong; Gilbert, David M; Bongiorni, Silvia; Prantera, Giorgio; Fanti, Laura; Pimpinelli, Sergio; Shi, Wei; Fundele, Reinald; Singh, Prim B.

In: Journal of Cell Science, Vol. 117, No. Pt 12, 15.05.2004, p. 2491-501.

Research output: Contribution to journalArticle

Kourmouli, N, Jeppesen, P, Mahadevhaiah, S, Burgoyne, P, Wu, R, Gilbert, DM, Bongiorni, S, Prantera, G, Fanti, L, Pimpinelli, S, Shi, W, Fundele, R & Singh, PB 2004, 'Heterochromatin and tri-methylated lysine 20 of histone H4 in animals', Journal of Cell Science, vol. 117, no. Pt 12, pp. 2491-501. https://doi.org/10.1242/jcs.01238
Kourmouli N, Jeppesen P, Mahadevhaiah S, Burgoyne P, Wu R, Gilbert DM et al. Heterochromatin and tri-methylated lysine 20 of histone H4 in animals. Journal of Cell Science. 2004 May 15;117(Pt 12):2491-501. https://doi.org/10.1242/jcs.01238
Kourmouli, Niki ; Jeppesen, Peter ; Mahadevhaiah, Shantha ; Burgoyne, Paul ; Wu, Rong ; Gilbert, David M ; Bongiorni, Silvia ; Prantera, Giorgio ; Fanti, Laura ; Pimpinelli, Sergio ; Shi, Wei ; Fundele, Reinald ; Singh, Prim B. / Heterochromatin and tri-methylated lysine 20 of histone H4 in animals. In: Journal of Cell Science. 2004 ; Vol. 117, No. Pt 12. pp. 2491-501.
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AU - Kourmouli, Niki

AU - Jeppesen, Peter

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AU - Wu, Rong

AU - Gilbert, David M

AU - Bongiorni, Silvia

AU - Prantera, Giorgio

AU - Fanti, Laura

AU - Pimpinelli, Sergio

AU - Shi, Wei

AU - Fundele, Reinald

AU - Singh, Prim B

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AB - Tri-methylated lysine 20 on histone H4 (Me(3)K20H4) is a marker of constitutive heterochromatin in murine interphase and metaphase cells. Heterochromatin marked by Me(3)K20H4 replicates late during S phase of the cell cycle. Serum starvation increases the number of cells that exhibit high levels of Me(3)K20H4 at constitutive heterochromatin. Me(3)K20H4 is also present at the centromeric heterochromatin of most meiotic chromosomes during spermatogenesis and at the pseudoautosomal region, as well as at some telomeres. It is not present on the XY-body. During murine embryogenesis the maternal pronucleus contains Me(3)K20H4; Me(3)K20H4 is absent from the paternal pronucleus. On Drosophila polytene chromosomes Me(3)K20H4 is present in a 'punctate pattern' at many chromosomal bands, including the chromocenter. In coccids it is present on the facultatively heterochromatinised paternal chromosome set. We also present evidence that Me(3)K20H4 is dependent upon H3-specific Suv(3)9 histone methyltransferase activity, suggesting that there may be 'epigenetic cross-talk' between histones H3 and H4.

KW - Animals

KW - Cell Line

KW - Cell Nucleus

KW - Cells, Cultured

KW - Centromere

KW - Chromosomes

KW - Culture Media, Serum-Free

KW - DNA

KW - Drosophila

KW - Embryo, Mammalian

KW - Embryo, Nonmammalian

KW - Female

KW - Fibroblasts

KW - Fluorescent Antibody Technique, Indirect

KW - Genetic Markers

KW - Hemiptera

KW - Heterochromatin

KW - Histones

KW - Lysine

KW - Male

KW - Methylation

KW - Mice

KW - Microscopy, Fluorescence

KW - Oocytes

KW - S Phase

KW - Spermatozoa

KW - Telomere

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1242/jcs.01238

DO - 10.1242/jcs.01238

M3 - Article

VL - 117

SP - 2491

EP - 2501

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - Pt 12

ER -