Abstract
We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 22-36 |
| Number of pages | 15 |
| Journal | Chromosoma |
| Volume | 111 |
| Issue number | 1 |
| Publication status | Published - Mar 2002 |
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Keywords
- Animals
- Bisbenzimidazole
- Chromosomal Proteins, Non-Histone
- DNA
- Drosophila
- Female
- Fluorescent Antibody Technique
- Gene Silencing
- Genomic Imprinting
- Heterochromatin
- Histones
- Lysine
- Male
- Methylation
- Mice
- Microscopy, Fluorescence
- Oocytes
- Spermatozoa
- Journal Article
- Research Support, Non-U.S. Gov't
Cite this
Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. / Cowell, Ian G; Aucott, Rebecca; Mahadevaiah, Shantha K; Burgoyne, Paul S; Huskisson, Neville; Bongiorni, Silvia; Prantera, Giorgio; Fanti, Laura; Pimpinelli, Sergio; Wu, Rong; Gilbert, David M; Shi, Wei; Fundele, Reinald; Morrison, Harris; Jeppesen, Peter; Singh, Prim B.
In: Chromosoma, Vol. 111, No. 1, 03.2002, p. 22-36.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals
AU - Cowell, Ian G
AU - Aucott, Rebecca
AU - Mahadevaiah, Shantha K
AU - Burgoyne, Paul S
AU - Huskisson, Neville
AU - Bongiorni, Silvia
AU - Prantera, Giorgio
AU - Fanti, Laura
AU - Pimpinelli, Sergio
AU - Wu, Rong
AU - Gilbert, David M
AU - Shi, Wei
AU - Fundele, Reinald
AU - Morrison, Harris
AU - Jeppesen, Peter
AU - Singh, Prim B
PY - 2002/3
Y1 - 2002/3
N2 - We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.
AB - We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.
KW - Animals
KW - Bisbenzimidazole
KW - Chromosomal Proteins, Non-Histone
KW - DNA
KW - Drosophila
KW - Female
KW - Fluorescent Antibody Technique
KW - Gene Silencing
KW - Genomic Imprinting
KW - Heterochromatin
KW - Histones
KW - Lysine
KW - Male
KW - Methylation
KW - Mice
KW - Microscopy, Fluorescence
KW - Oocytes
KW - Spermatozoa
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Article
VL - 111
SP - 22
EP - 36
JO - Chromosoma
JF - Chromosoma
SN - 0009-5915
IS - 1
ER -