Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals

Ian G Cowell, Rebecca Aucott, Shantha K Mahadevaiah, Paul S Burgoyne, Neville Huskisson, Silvia Bongiorni, Giorgio Prantera, Laura Fanti, Sergio Pimpinelli, Rong Wu, David M Gilbert, Wei Shi, Reinald Fundele, Harris Morrison, Peter Jeppesen, Prim B Singh

Research output: Contribution to journalArticle

218 Citations (Scopus)

Abstract

We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.

Original languageEnglish
Pages (from-to)22-36
Number of pages15
JournalChromosoma
Volume111
Issue number1
Publication statusPublished - Mar 2002

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Heterochromatin
Histones
Methylation
Lysine
Chromosomes
Mammalian Chromosomes
Meiosis
X Chromosome
S Phase
Cell Cycle
Embryonic Structures
Mothers
DNA
heterochromatin-specific nonhistone chromosomal protein HP-1

Keywords

  • Animals
  • Bisbenzimidazole
  • Chromosomal Proteins, Non-Histone
  • DNA
  • Drosophila
  • Female
  • Fluorescent Antibody Technique
  • Gene Silencing
  • Genomic Imprinting
  • Heterochromatin
  • Histones
  • Lysine
  • Male
  • Methylation
  • Mice
  • Microscopy, Fluorescence
  • Oocytes
  • Spermatozoa
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Cowell, I. G., Aucott, R., Mahadevaiah, S. K., Burgoyne, P. S., Huskisson, N., Bongiorni, S., ... Singh, P. B. (2002). Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. Chromosoma, 111(1), 22-36.

Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. / Cowell, Ian G; Aucott, Rebecca; Mahadevaiah, Shantha K; Burgoyne, Paul S; Huskisson, Neville; Bongiorni, Silvia; Prantera, Giorgio; Fanti, Laura; Pimpinelli, Sergio; Wu, Rong; Gilbert, David M; Shi, Wei; Fundele, Reinald; Morrison, Harris; Jeppesen, Peter; Singh, Prim B.

In: Chromosoma, Vol. 111, No. 1, 03.2002, p. 22-36.

Research output: Contribution to journalArticle

Cowell, IG, Aucott, R, Mahadevaiah, SK, Burgoyne, PS, Huskisson, N, Bongiorni, S, Prantera, G, Fanti, L, Pimpinelli, S, Wu, R, Gilbert, DM, Shi, W, Fundele, R, Morrison, H, Jeppesen, P & Singh, PB 2002, 'Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals', Chromosoma, vol. 111, no. 1, pp. 22-36.
Cowell IG, Aucott R, Mahadevaiah SK, Burgoyne PS, Huskisson N, Bongiorni S et al. Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. Chromosoma. 2002 Mar;111(1):22-36.
Cowell, Ian G ; Aucott, Rebecca ; Mahadevaiah, Shantha K ; Burgoyne, Paul S ; Huskisson, Neville ; Bongiorni, Silvia ; Prantera, Giorgio ; Fanti, Laura ; Pimpinelli, Sergio ; Wu, Rong ; Gilbert, David M ; Shi, Wei ; Fundele, Reinald ; Morrison, Harris ; Jeppesen, Peter ; Singh, Prim B. / Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. In: Chromosoma. 2002 ; Vol. 111, No. 1. pp. 22-36.
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AU - Gilbert, David M

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