Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals

Ian G Cowell; Rebecca Aucott; Shantha K Mahadevaiah; Paul S Burgoyne; Neville Huskisson; Silvia Bongiorni; Giorgio Prantera; Laura Fanti; Sergio Pimpinelli; Rong Wu; David M Gilbert; Wei Shi; Reinald Fundele; Harris Morrison; Peter Jeppesen; Prim B Singh

Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals

Ian G Cowell, Rebecca Aucott, Shantha K Mahadevaiah, Paul S Burgoyne, Neville Huskisson, Silvia Bongiorni, Giorgio Prantera, Laura Fanti, Sergio Pimpinelli, Rong Wu, David M Gilbert, Wei Shi, Reinald Fundele, Harris Morrison, Peter Jeppesen, Prim B Singh

Department of Medicine

Research output: Contribution to journal › Article

220 Citations (Scopus)

Abstract

We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.

Original language	English
Pages (from-to)	22-36
Number of pages	15
Journal	Chromosoma
Volume	111
Issue number	1
Publication status	Published - Mar 2002

Keywords

Animals
Bisbenzimidazole
Chromosomal Proteins, Non-Histone
DNA
Drosophila
Female
Fluorescent Antibody Technique
Gene Silencing
Genomic Imprinting
Heterochromatin
Histones
Lysine
Male
Methylation
Mice
Microscopy, Fluorescence
Oocytes
Spermatozoa
Journal Article
Research Support, Non-U.S. Gov't

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Cowell, I. G., Aucott, R., Mahadevaiah, S. K., Burgoyne, P. S., Huskisson, N., Bongiorni, S., Prantera, G., Fanti, L., Pimpinelli, S., Wu, R., Gilbert, D. M., Shi, W., Fundele, R., Morrison, H., Jeppesen, P., & Singh, P. B. (2002). Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. Chromosoma, 111(1), 22-36.

Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. / Cowell, Ian G; Aucott, Rebecca; Mahadevaiah, Shantha K; Burgoyne, Paul S; Huskisson, Neville; Bongiorni, Silvia; Prantera, Giorgio; Fanti, Laura; Pimpinelli, Sergio; Wu, Rong; Gilbert, David M; Shi, Wei; Fundele, Reinald; Morrison, Harris; Jeppesen, Peter; Singh, Prim B.

In: Chromosoma, Vol. 111, No. 1, 03.2002, p. 22-36.

Research output: Contribution to journal › Article

Cowell, Ian G ; Aucott, Rebecca ; Mahadevaiah, Shantha K ; Burgoyne, Paul S ; Huskisson, Neville ; Bongiorni, Silvia ; Prantera, Giorgio ; Fanti, Laura ; Pimpinelli, Sergio ; Wu, Rong ; Gilbert, David M ; Shi, Wei ; Fundele, Reinald ; Morrison, Harris ; Jeppesen, Peter ; Singh, Prim B. / Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals. In: Chromosoma. 2002 ; Vol. 111, No. 1. pp. 22-36.

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abstract = "We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.",

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AU - Cowell, Ian G

AU - Aucott, Rebecca

AU - Mahadevaiah, Shantha K

AU - Burgoyne, Paul S

AU - Huskisson, Neville

AU - Bongiorni, Silvia

AU - Prantera, Giorgio

AU - Fanti, Laura

AU - Pimpinelli, Sergio

AU - Wu, Rong

AU - Gilbert, David M

AU - Shi, Wei

AU - Fundele, Reinald

AU - Morrison, Harris

AU - Jeppesen, Peter

AU - Singh, Prim B

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N2 - We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.

AB - We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.

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KW - Bisbenzimidazole

KW - Chromosomal Proteins, Non-Histone

KW - DNA

KW - Drosophila

KW - Female

KW - Fluorescent Antibody Technique

KW - Gene Silencing

KW - Genomic Imprinting

KW - Heterochromatin

KW - Histones

KW - Lysine

KW - Male

KW - Methylation

KW - Mice

KW - Microscopy, Fluorescence

KW - Oocytes

KW - Spermatozoa

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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JO - Chromosoma

JF - Chromosoma

SN - 0009-5915

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