Abstract
We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.
Original language | English |
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Pages (from-to) | 22-36 |
Number of pages | 15 |
Journal | Chromosoma |
Volume | 111 |
Issue number | 1 |
Publication status | Published - Mar 2002 |
Keywords
- Animals
- Bisbenzimidazole
- Chromosomal Proteins, Non-Histone
- DNA
- Drosophila
- Female
- Fluorescent Antibody Technique
- Gene Silencing
- Genomic Imprinting
- Heterochromatin
- Histones
- Lysine
- Male
- Methylation
- Mice
- Microscopy, Fluorescence
- Oocytes
- Spermatozoa
- Journal Article
- Research Support, Non-U.S. Gov't