Highly efficient mRNA-based gene transfer in feeder-free cultured H9 human embryonic stem cells

Peter Ponsaerts, Sjaak van der Sar, Viggo F I Van Tendeloo, Philippe G Jorens, Zwi N Berneman, Prim B Singh

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Several protocols have been described for virus-based gene transfer in human embryonic stem (hES) cells, while efficient non-viral methods are currently non-existing. In this study, we investigated the efficiency of mRNA-based gene transfer in feeder-free cultured H9 hES cells, based on electroporation of in vitro transcribed mRNA encoding the enhanced green fluorescent protein (EGFP). Optimisation of culture and electroporation conditions for feeder-free cultured H9 hES cells resulted a highly pure, transgene-expressing (90% positive cells) H9 hES cell population.

Original languageEnglish
Pages (from-to)211-6
Number of pages6
JournalCellular Reprogramming
Volume6
Issue number3
DOIs
Publication statusPublished - 2004

Keywords

  • Cell Survival
  • Cells, Cultured
  • Electroporation
  • Flow Cytometry
  • Green Fluorescent Proteins
  • Humans
  • Stem Cells
  • Transfection
  • Journal Article
  • Research Support, Non-U.S. Gov't

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    Ponsaerts, P., van der Sar, S., Van Tendeloo, V. F. I., Jorens, P. G., Berneman, Z. N., & Singh, P. B. (2004). Highly efficient mRNA-based gene transfer in feeder-free cultured H9 human embryonic stem cells. Cellular Reprogramming, 6(3), 211-6. https://doi.org/10.1089/clo.2004.6.211