Abstract
Several protocols have been described for virus-based gene transfer in human embryonic stem (hES) cells, while efficient non-viral methods are currently non-existing. In this study, we investigated the efficiency of mRNA-based gene transfer in feeder-free cultured H9 hES cells, based on electroporation of in vitro transcribed mRNA encoding the enhanced green fluorescent protein (EGFP). Optimisation of culture and electroporation conditions for feeder-free cultured H9 hES cells resulted a highly pure, transgene-expressing (90% positive cells) H9 hES cell population.
Original language | English |
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Pages (from-to) | 211-6 |
Number of pages | 6 |
Journal | Cellular Reprogramming |
Volume | 6 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2004 |
Keywords
- Cell Survival
- Cells, Cultured
- Electroporation
- Flow Cytometry
- Green Fluorescent Proteins
- Humans
- Stem Cells
- Transfection
- Journal Article
- Research Support, Non-U.S. Gov't