Highly efficient mRNA-based gene transfer in feeder-free cultured H9 human embryonic stem cells

Peter Ponsaerts, Sjaak van der Sar, Viggo F I Van Tendeloo, Philippe G Jorens, Zwi N Berneman, Prim B Singh

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Several protocols have been described for virus-based gene transfer in human embryonic stem (hES) cells, while efficient non-viral methods are currently non-existing. In this study, we investigated the efficiency of mRNA-based gene transfer in feeder-free cultured H9 hES cells, based on electroporation of in vitro transcribed mRNA encoding the enhanced green fluorescent protein (EGFP). Optimisation of culture and electroporation conditions for feeder-free cultured H9 hES cells resulted a highly pure, transgene-expressing (90% positive cells) H9 hES cell population.

Original languageEnglish
Pages (from-to)211-6
Number of pages6
JournalCellular Reprogramming
Volume6
Issue number3
DOIs
Publication statusPublished - 2004

Fingerprint

Messenger RNA
Electroporation
Genes
Transgenes
Viruses
Human Embryonic Stem Cells
Population
enhanced green fluorescent protein
In Vitro Techniques

Keywords

  • Cell Survival
  • Cells, Cultured
  • Electroporation
  • Flow Cytometry
  • Green Fluorescent Proteins
  • Humans
  • Stem Cells
  • Transfection
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Highly efficient mRNA-based gene transfer in feeder-free cultured H9 human embryonic stem cells. / Ponsaerts, Peter; van der Sar, Sjaak; Van Tendeloo, Viggo F I; Jorens, Philippe G; Berneman, Zwi N; Singh, Prim B.

In: Cellular Reprogramming, Vol. 6, No. 3, 2004, p. 211-6.

Research output: Contribution to journalArticle

Ponsaerts, P, van der Sar, S, Van Tendeloo, VFI, Jorens, PG, Berneman, ZN & Singh, PB 2004, 'Highly efficient mRNA-based gene transfer in feeder-free cultured H9 human embryonic stem cells', Cellular Reprogramming, vol. 6, no. 3, pp. 211-6. https://doi.org/10.1089/clo.2004.6.211
Ponsaerts, Peter ; van der Sar, Sjaak ; Van Tendeloo, Viggo F I ; Jorens, Philippe G ; Berneman, Zwi N ; Singh, Prim B. / Highly efficient mRNA-based gene transfer in feeder-free cultured H9 human embryonic stem cells. In: Cellular Reprogramming. 2004 ; Vol. 6, No. 3. pp. 211-6.
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