Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1

H Polioudaki, N Kourmouli, V Drosou, A Bakou, P A Theodoropoulos, P B Singh, T Giannakouros, S D Georgatos

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

We have recently shown that heterochromatin protein 1 (HP1) interacts with the nuclear envelope in an acetylation-dependent manner. Using purified components and in vitro assays, we now demonstrate that HP1 forms a quaternary complex with the inner nuclear membrane protein LBR and a sub-set of core histones. This complex involves histone H3/H4 oligomers, which mediate binding of LBR to HP1 and cross-link these two proteins that do not interact directly with each other. Consistent with previous observations, HP1 and LBR binding to core histones is strongly inhibited when H3/H4 are modified by recombinant CREB-binding protein, revealing a new mechanism for anchoring domains of under-acetylated chromatin to the inner nuclear membrane.

Original languageEnglish
Pages (from-to)920-5
Number of pages6
JournalEMBO Reports
Volume2
Issue number10
DOIs
Publication statusPublished - Oct 2001

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Nuclear Envelope
Nuclear Proteins
Histones
Membrane Proteins
CREB-Binding Protein
Acetylation
Recombinant Proteins
Oligomers
Chromatin
Assays
Membranes
heterochromatin-specific nonhistone chromosomal protein HP-1
Proteins

Keywords

  • Acetylation
  • Animals
  • Binding Sites
  • Blotting, Western
  • Cell Nucleus
  • Dose-Response Relationship, Drug
  • Electrophoresis, Polyacrylamide Gel
  • Fishes
  • Glutathione Transferase
  • Heterochromatin
  • Histones
  • Intracellular Membranes
  • Mice
  • Models, Biological
  • Plasmids
  • Precipitin Tests
  • Protein Binding
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Turkeys
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Polioudaki, H., Kourmouli, N., Drosou, V., Bakou, A., Theodoropoulos, P. A., Singh, P. B., ... Georgatos, S. D. (2001). Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1. EMBO Reports, 2(10), 920-5. https://doi.org/10.1093/embo-reports/kve199

Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1. / Polioudaki, H; Kourmouli, N; Drosou, V; Bakou, A; Theodoropoulos, P A; Singh, P B; Giannakouros, T; Georgatos, S D.

In: EMBO Reports, Vol. 2, No. 10, 10.2001, p. 920-5.

Research output: Contribution to journalArticle

Polioudaki, H, Kourmouli, N, Drosou, V, Bakou, A, Theodoropoulos, PA, Singh, PB, Giannakouros, T & Georgatos, SD 2001, 'Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1', EMBO Reports, vol. 2, no. 10, pp. 920-5. https://doi.org/10.1093/embo-reports/kve199
Polioudaki, H ; Kourmouli, N ; Drosou, V ; Bakou, A ; Theodoropoulos, P A ; Singh, P B ; Giannakouros, T ; Georgatos, S D. / Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1. In: EMBO Reports. 2001 ; Vol. 2, No. 10. pp. 920-5.
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KW - Binding Sites

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KW - Turkeys

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