Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1

H Polioudaki, N Kourmouli, V Drosou, A Bakou, P A Theodoropoulos, P B Singh, T Giannakouros, S D Georgatos

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

We have recently shown that heterochromatin protein 1 (HP1) interacts with the nuclear envelope in an acetylation-dependent manner. Using purified components and in vitro assays, we now demonstrate that HP1 forms a quaternary complex with the inner nuclear membrane protein LBR and a sub-set of core histones. This complex involves histone H3/H4 oligomers, which mediate binding of LBR to HP1 and cross-link these two proteins that do not interact directly with each other. Consistent with previous observations, HP1 and LBR binding to core histones is strongly inhibited when H3/H4 are modified by recombinant CREB-binding protein, revealing a new mechanism for anchoring domains of under-acetylated chromatin to the inner nuclear membrane.

Original languageEnglish
Pages (from-to)920-5
Number of pages6
JournalEMBO Reports
Volume2
Issue number10
DOIs
Publication statusPublished - Oct 2001

Keywords

  • Acetylation
  • Animals
  • Binding Sites
  • Blotting, Western
  • Cell Nucleus
  • Dose-Response Relationship, Drug
  • Electrophoresis, Polyacrylamide Gel
  • Fishes
  • Glutathione Transferase
  • Heterochromatin
  • Histones
  • Intracellular Membranes
  • Mice
  • Models, Biological
  • Plasmids
  • Precipitin Tests
  • Protein Binding
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Turkeys
  • Journal Article
  • Research Support, Non-U.S. Gov't

Fingerprint Dive into the research topics of 'Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1'. Together they form a unique fingerprint.

  • Cite this

    Polioudaki, H., Kourmouli, N., Drosou, V., Bakou, A., Theodoropoulos, P. A., Singh, P. B., Giannakouros, T., & Georgatos, S. D. (2001). Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1. EMBO Reports, 2(10), 920-5. https://doi.org/10.1093/embo-reports/kve199