HP1-beta is required for development of the cerebral neocortex and neuromuscular junctions

Rebecca Aucott, Jörn Bullwinkel, Yang Yu, Wei Shi, Mustafa Billur, Jeremy P Brown, Ursula Menzel, Dimitris Kioussis, Guozheng Wang, Ingrid Reisert, Jörg Weimer, Raj K Pandita, Girdhar G Sharma, Tej K Pandita, Reinald Fundele, Prim B Singh

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69 Citations (Scopus)

Abstract

HP1 proteins are thought to be modulators of chromatin organization in all mammals, yet their exact physiological function remains unknown. In a first attempt to elucidate the function of these proteins in vivo, we disrupted the murine Cbx1 gene, which encodes the HP1-beta isotype, and show that the Cbx1(-/-) -null mutation leads to perinatal lethality. The newborn mice succumbed to acute respiratory failure, whose likely cause is the defective development of neuromuscular junctions within the endplate of the diaphragm. We also observe aberrant cerebral cortex development in Cbx1(-/-) mutant brains, which have reduced proliferation of neuronal precursors, widespread cell death, and edema. In vitro cultures of neurospheres from Cbx1(-/-) mutant brains reveal a dramatic genomic instability. Our results demonstrate that HP1 proteins are not functionally redundant and that they are likely to regulate lineage-specific changes in heterochromatin organization.

Original languageEnglish
Pages (from-to)597-606
Number of pages10
JournalJournal of Cell Biology
Volume183
Issue number4
DOIs
Publication statusPublished - Nov 17 2008

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Keywords

  • Animals
  • Chromosomal Proteins, Non-Histone
  • Diaphragm
  • Genomic Instability
  • Heterochromatin
  • Humans
  • Mice
  • Mice, Knockout
  • Motor Endplate
  • Neocortex
  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

Cite this

Aucott, R., Bullwinkel, J., Yu, Y., Shi, W., Billur, M., Brown, J. P., Menzel, U., Kioussis, D., Wang, G., Reisert, I., Weimer, J., Pandita, R. K., Sharma, G. G., Pandita, T. K., Fundele, R., & Singh, P. B. (2008). HP1-beta is required for development of the cerebral neocortex and neuromuscular junctions. Journal of Cell Biology, 183(4), 597-606. https://doi.org/10.1083/jcb.200804041