Human c-myc gene contains a regulatory site similar to consensus of interferon response sequence (IRS)

N. M. Alexandrova, A. V. Itkes, L. R. Imamova, B. K. Chernov, E. M. Tulchinsky, N. B. Ulyanov, L. L. Kisselev

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Expression of c-myc proto oncogene is regulated by multiple mechanisms. Here, we report that the consensus of the regulatory region of interferondependent genes, GGAAAN1-3 GAAA, was found after computer search in the 5'-terminal flank of human c-myc gene in position (-76:-67). In vitro transcription of c-myc gene fragments showed that the consensus region competes with oligonucleotide GGGAAAATGAAACT for binding to specific protein(s). This oligonucleotide was shown to bind selectively the interferon-dependent positive transcription factor [1]. Transcription of c-myc fragments lacking 5'-terminal region up to positions -101 or +71 was initiated at two sites located in the first intron. These sites did not coincide with P1 in vivo RNA cap-site. Binding of the protein factor(s) to the regulatory region of c-myc gene -76:-67 blocked the in vitro transcription initiated in the first intron.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalFEBS Letters
Volume265
Issue number1-2
DOIs
Publication statusPublished - Jun 4 1990
Externally publishedYes

Keywords

  • c-myc gene
  • Interferon-dependent gene
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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