Human c-myc gene contains a regulatory site similar to consensus of interferon response sequence (IRS)

N. M. Alexandrova; A. V. Itkes; L. R. Imamova; B. K. Chernov; E. M. Tulchinsky; N. B. Ulyanov; L. L. Kisselev

doi:10.1016/0014-5793(90)80885-M

Human c-myc gene contains a regulatory site similar to consensus of interferon response sequence (IRS)

N. M. Alexandrova, A. V. Itkes, L. R. Imamova, B. K. Chernov, E. M. Tulchinsky, N. B. Ulyanov, L. L. Kisselev

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Expression of c-myc proto oncogene is regulated by multiple mechanisms. Here, we report that the consensus of the regulatory region of interferondependent genes, GGAAAN_1-3 GAAA, was found after computer search in the 5'-terminal flank of human c-myc gene in position (-76:-67). In vitro transcription of c-myc gene fragments showed that the consensus region competes with oligonucleotide GGGAAAATGAAACT for binding to specific protein(s). This oligonucleotide was shown to bind selectively the interferon-dependent positive transcription factor [1]. Transcription of c-myc fragments lacking 5'-terminal region up to positions -101 or +71 was initiated at two sites located in the first intron. These sites did not coincide with P₁ in vivo RNA cap-site. Binding of the protein factor(s) to the regulatory region of c-myc gene -76:-67 blocked the in vitro transcription initiated in the first intron.

Original language	English
Pages (from-to)	67-70
Number of pages	4
Journal	FEBS Letters
Volume	265
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(90)80885-M
Publication status	Published - Jun 4 1990
Externally published	Yes

Keywords

c-myc gene
Interferon-dependent gene
Transcription

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Human c-myc gene contains a regulatory site similar to consensus of interferon response sequence (IRS)",

abstract = "Expression of c-myc proto oncogene is regulated by multiple mechanisms. Here, we report that the consensus of the regulatory region of interferondependent genes, GGAAAN1-3 GAAA, was found after computer search in the 5'-terminal flank of human c-myc gene in position (-76:-67). In vitro transcription of c-myc gene fragments showed that the consensus region competes with oligonucleotide GGGAAAATGAAACT for binding to specific protein(s). This oligonucleotide was shown to bind selectively the interferon-dependent positive transcription factor [1]. Transcription of c-myc fragments lacking 5'-terminal region up to positions -101 or +71 was initiated at two sites located in the first intron. These sites did not coincide with P1 in vivo RNA cap-site. Binding of the protein factor(s) to the regulatory region of c-myc gene -76:-67 blocked the in vitro transcription initiated in the first intron.",

keywords = "c-myc gene, Interferon-dependent gene, Transcription",

author = "Alexandrova, {N. M.} and Itkes, {A. V.} and Imamova, {L. R.} and Chernov, {B. K.} and Tulchinsky, {E. M.} and Ulyanov, {N. B.} and Kisselev, {L. L.}",

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doi = "10.1016/0014-5793(90)80885-M",

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T1 - Human c-myc gene contains a regulatory site similar to consensus of interferon response sequence (IRS)

AU - Alexandrova, N. M.

AU - Itkes, A. V.

AU - Imamova, L. R.

AU - Chernov, B. K.

AU - Tulchinsky, E. M.

AU - Ulyanov, N. B.

AU - Kisselev, L. L.

PY - 1990/6/4

Y1 - 1990/6/4

N2 - Expression of c-myc proto oncogene is regulated by multiple mechanisms. Here, we report that the consensus of the regulatory region of interferondependent genes, GGAAAN1-3 GAAA, was found after computer search in the 5'-terminal flank of human c-myc gene in position (-76:-67). In vitro transcription of c-myc gene fragments showed that the consensus region competes with oligonucleotide GGGAAAATGAAACT for binding to specific protein(s). This oligonucleotide was shown to bind selectively the interferon-dependent positive transcription factor [1]. Transcription of c-myc fragments lacking 5'-terminal region up to positions -101 or +71 was initiated at two sites located in the first intron. These sites did not coincide with P1 in vivo RNA cap-site. Binding of the protein factor(s) to the regulatory region of c-myc gene -76:-67 blocked the in vitro transcription initiated in the first intron.

AB - Expression of c-myc proto oncogene is regulated by multiple mechanisms. Here, we report that the consensus of the regulatory region of interferondependent genes, GGAAAN1-3 GAAA, was found after computer search in the 5'-terminal flank of human c-myc gene in position (-76:-67). In vitro transcription of c-myc gene fragments showed that the consensus region competes with oligonucleotide GGGAAAATGAAACT for binding to specific protein(s). This oligonucleotide was shown to bind selectively the interferon-dependent positive transcription factor [1]. Transcription of c-myc fragments lacking 5'-terminal region up to positions -101 or +71 was initiated at two sites located in the first intron. These sites did not coincide with P1 in vivo RNA cap-site. Binding of the protein factor(s) to the regulatory region of c-myc gene -76:-67 blocked the in vitro transcription initiated in the first intron.

KW - c-myc gene

KW - Interferon-dependent gene

KW - Transcription

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JF - FEBS Letters

SN - 0014-5793

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ER -

Human c-myc gene contains a regulatory site similar to consensus of interferon response sequence (IRS)

Abstract

Keywords

ASJC Scopus subject areas

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