Abstract
Expression of c-myc proto oncogene is regulated by multiple mechanisms. Here, we report that the consensus of the regulatory region of interferondependent genes, GGAAAN1-3 GAAA, was found after computer search in the 5'-terminal flank of human c-myc gene in position (-76:-67). In vitro transcription of c-myc gene fragments showed that the consensus region competes with oligonucleotide GGGAAAATGAAACT for binding to specific protein(s). This oligonucleotide was shown to bind selectively the interferon-dependent positive transcription factor [1]. Transcription of c-myc fragments lacking 5'-terminal region up to positions -101 or +71 was initiated at two sites located in the first intron. These sites did not coincide with P1 in vivo RNA cap-site. Binding of the protein factor(s) to the regulatory region of c-myc gene -76:-67 blocked the in vitro transcription initiated in the first intron.
Original language | English |
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Pages (from-to) | 67-70 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 265 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - Jun 4 1990 |
Externally published | Yes |
Keywords
- c-myc gene
- Interferon-dependent gene
- Transcription
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology