Human papillomavirus subtype 16 is common in Pakistani women with cervical carcinoma

Saeed Khan, Nadeem N. Jaffer, Mohammad Nasherwan Khan, Mohammad A. Rai, Majid Shafiq, Adnan Ali, Shahid Pervez, Nusrat Khan, Aliya Aziz, Syed H. Ali

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Introduction: Human papillomavirus (HPV) is recognized as a major causative agent for cervical carcinomas. Based on their oncogenic potential, HPV subtypes have been divided into high- and low-risk. In Pakistan, screening for HPV in female patients is not commonly practiced, and as a consequence, the degree of HPV prevalence and its correlation with cervical cancer is unknown. Objective: In this study, we have attempted to estimate the prevalence of HPV infection, and also the HPV subtype profile, among Pakistani women with cervical cancer from varied geographical, racial, and social backgrounds within Pakistan. Methodology: Women visiting two tertiary care hospitals in Karachi, diagnosed with carcinoma of the cervix within the past 15 years, were analyzed for HPV subtypes in their cancer specimens. Retrospectively, 60 paraffin-embedded cervical cancer biopsies were examined for the presence of HPV DNA. After DNA extraction from these samples, polymerase chain reaction (PCR) was used to amplify the HPV L1 gene using the consensus (general) primers, and primers specific for subtypes 16 and 18. Results: Of the 60 samples analyzed, only one sample was HPV negative; the rest of the samples were positive for the presence of HPV. Of the 59 HPV positive samples, 56 showed the presence of HPV16 and one sample was positive for HPV18; HPV subtype could not be determined in two samples. Conclusion: Our results show a strong relationship between HPV infection and cervical cancer among Pakistani women. These results underscore the need to implement regular HPV screening for Pakistani women. An early diagnosis of HPV infection will allow better health management to reduce the risk of developing cervical cancer.

Original languageEnglish
Pages (from-to)313-317
Number of pages5
JournalInternational Journal of Infectious Diseases
Volume11
Issue number4
DOIs
Publication statusPublished - Jul 2007
Externally publishedYes

Fingerprint

Human papillomavirus 16
Carcinoma
Uterine Cervical Neoplasms
Papillomavirus Infections
Pakistan
DNA
Tertiary Healthcare
Tertiary Care Centers
Cervix Uteri
Paraffin

Keywords

  • Cervical cancer
  • Epidemiology
  • HPV
  • Pakistan
  • Subtypes

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Human papillomavirus subtype 16 is common in Pakistani women with cervical carcinoma. / Khan, Saeed; Jaffer, Nadeem N.; Khan, Mohammad Nasherwan; Rai, Mohammad A.; Shafiq, Majid; Ali, Adnan; Pervez, Shahid; Khan, Nusrat; Aziz, Aliya; Ali, Syed H.

In: International Journal of Infectious Diseases, Vol. 11, No. 4, 07.2007, p. 313-317.

Research output: Contribution to journalArticle

Khan, S, Jaffer, NN, Khan, MN, Rai, MA, Shafiq, M, Ali, A, Pervez, S, Khan, N, Aziz, A & Ali, SH 2007, 'Human papillomavirus subtype 16 is common in Pakistani women with cervical carcinoma', International Journal of Infectious Diseases, vol. 11, no. 4, pp. 313-317. https://doi.org/10.1016/j.ijid.2006.06.007
Khan, Saeed ; Jaffer, Nadeem N. ; Khan, Mohammad Nasherwan ; Rai, Mohammad A. ; Shafiq, Majid ; Ali, Adnan ; Pervez, Shahid ; Khan, Nusrat ; Aziz, Aliya ; Ali, Syed H. / Human papillomavirus subtype 16 is common in Pakistani women with cervical carcinoma. In: International Journal of Infectious Diseases. 2007 ; Vol. 11, No. 4. pp. 313-317.
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