Abstract
Various methods of measuring the pH within particles of small size are reviewed. In relation to the chloroplasts the authors analyse the possible reasons for the inadequacy of the measurement of transmembrane pH difference (Δ pH) from the distribution of the pH indicators within and outside the vesicles. From analysis of the published data they show that the divergences appearing on measuring Δ pH by different methods may be due not only to the binding of the pH indicators to the membrane but also the heterogeneity (morphological and functional) of the system. The question of the physical meaning of the concept of pH within subcellular particles of small size is examined.
| Original language | English |
|---|---|
| Pages (from-to) | 581-594 |
| Number of pages | 14 |
| Journal | Biophysics |
| Volume | 30 |
| Issue number | 3 |
| Publication status | Published - 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biophysics
Cite this
Hydrogen ion concentration in subcellular particles : Physical meaning and methods of determination. / Tikhonov, A. N.; Blyumenfel'd, L. A.
In: Biophysics, Vol. 30, No. 3, 1985, p. 581-594.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hydrogen ion concentration in subcellular particles
T2 - Physical meaning and methods of determination
AU - Tikhonov, A. N.
AU - Blyumenfel'd, L. A.
PY - 1985
Y1 - 1985
N2 - Various methods of measuring the pH within particles of small size are reviewed. In relation to the chloroplasts the authors analyse the possible reasons for the inadequacy of the measurement of transmembrane pH difference (Δ pH) from the distribution of the pH indicators within and outside the vesicles. From analysis of the published data they show that the divergences appearing on measuring Δ pH by different methods may be due not only to the binding of the pH indicators to the membrane but also the heterogeneity (morphological and functional) of the system. The question of the physical meaning of the concept of pH within subcellular particles of small size is examined.
AB - Various methods of measuring the pH within particles of small size are reviewed. In relation to the chloroplasts the authors analyse the possible reasons for the inadequacy of the measurement of transmembrane pH difference (Δ pH) from the distribution of the pH indicators within and outside the vesicles. From analysis of the published data they show that the divergences appearing on measuring Δ pH by different methods may be due not only to the binding of the pH indicators to the membrane but also the heterogeneity (morphological and functional) of the system. The question of the physical meaning of the concept of pH within subcellular particles of small size is examined.
UR - http://www.scopus.com/inward/record.url?scp=46549102887&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=46549102887&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:46549102887
VL - 30
SP - 581
EP - 594
JO - Biophysics (Russian Federation)
JF - Biophysics (Russian Federation)
SN - 0006-3509
IS - 3
ER -