IgE influences the number and function of mature mast cells, but not progenitor recruitment in allergic pulmonary inflammation

Clinton B. Mathias, Eva Jasmin Freyschmidt, Benjamin Caplan, Tatiana Jones, Dimitri Poddighe, Wei Xing, Krista L. Harrison, Michael F. Gurish, Hans C. Oettgen

Research output: Contribution to journalArticle

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Abstract

Studies performed using cultured cells indicate that IgE functions not only to trigger degranulation of mast cells following allergen exposure, but also to enhance their survival. Such an influence of IgE on mast cell homeostasis during allergic responses in vivo has not been established. In this study, we show that inhalation of Aspergillus fumigatus extract in mice induced a dramatic rise in IgE accompanied by an increase in airway mast cells. These had an activated phenotype with high levels of FcεRI. Plasma mast cell protease-1 was also increased, indicating an elevated systemic mast cell load. In addition, enhanced levels of IL-5 and eosinophils were observed in the airway. Both mast cell expansion and activation were markedly attenuated in IgE-/- animals that are incapable of producing IgE in response to A. fumigatus. The recruitment of eosinophils to the airways was also reduced in IgE-/- mice. Analyses of potential cellular targets of IgE revealed that IgE Abs are not required for the induction of mast cell progenitors in response to allergen, but rather act by sustaining the survival of mature mast cells. Our results identify an important role for IgE Abs in promoting mast cell expansion during allergic responses in vivo.

Original languageEnglish
Pages (from-to)2416-2424
Number of pages9
JournalJournal of Immunology
Volume182
Issue number4
DOIs
Publication statusPublished - Feb 15 2009
Externally publishedYes

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Mast Cells
Immunoglobulin E
Pneumonia
Aspergillus fumigatus
Eosinophils
Allergens
Interleukin-5
Plasma Cells
Inhalation
Cultured Cells
Homeostasis
Peptide Hydrolases
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

IgE influences the number and function of mature mast cells, but not progenitor recruitment in allergic pulmonary inflammation. / Mathias, Clinton B.; Freyschmidt, Eva Jasmin; Caplan, Benjamin; Jones, Tatiana; Poddighe, Dimitri; Xing, Wei; Harrison, Krista L.; Gurish, Michael F.; Oettgen, Hans C.

In: Journal of Immunology, Vol. 182, No. 4, 15.02.2009, p. 2416-2424.

Research output: Contribution to journalArticle

Mathias, CB, Freyschmidt, EJ, Caplan, B, Jones, T, Poddighe, D, Xing, W, Harrison, KL, Gurish, MF & Oettgen, HC 2009, 'IgE influences the number and function of mature mast cells, but not progenitor recruitment in allergic pulmonary inflammation', Journal of Immunology, vol. 182, no. 4, pp. 2416-2424. https://doi.org/10.4049/jimmunol.0801569
Mathias, Clinton B. ; Freyschmidt, Eva Jasmin ; Caplan, Benjamin ; Jones, Tatiana ; Poddighe, Dimitri ; Xing, Wei ; Harrison, Krista L. ; Gurish, Michael F. ; Oettgen, Hans C. / IgE influences the number and function of mature mast cells, but not progenitor recruitment in allergic pulmonary inflammation. In: Journal of Immunology. 2009 ; Vol. 182, No. 4. pp. 2416-2424.
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