IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling

Clinton B. Mathias, Suejy A. Hobson, Maria Garcia-Lloret, Greg Lawson, Dimitri Poddighe, Eva Jasmin Freyschmidt, Wei Xing, Michael F. Gurish, Talal A. Chatila, Hans C. Oettgen

Research output: Contribution to journalArticle

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Abstract

Background: In atopic subjects food ingestion drives the production of IgE antibodies that can trigger hypersensitivity reactions. The IL-4 pathway plays a critical role in this response, and genetic polymorphisms in its components have been linked to allergy. Objective: We sought to test whether an activating mutation in the IL-4 receptor (IL-4R) α chain enhances allergic responses to a food antigen. Methods: F709 mice, in which the IL-4Rα immunoreceptor tyrosine-based inhibitory motif is inactivated, were gavage fed with ovalbumin (OVA). Reactions to OVA challenge and immune responses, including antibody production and TH2 responses, were assessed. Results: F709 mice, but not wild-type control animals, sensitized by means of gavage with OVA and either cholera toxin or staphylococcal enterotoxin B, displayed mast cell activation and systemic anaphylaxis on enteral challenge. Anaphylaxis was elicited even in F709 mice enterally sensitized with OVA alone. Bone marrow chimera experiments established that the increased sensitivity conferred by the F709 genotype was mediated mostly by hematopoietic cells but that nonhematopoietic cells also contributed. F709 mice exhibited increased intestinal permeability to macromolecules. The F709 genotype conferred increased OVA-specific IgE but not IgG1 responses, local and systemic TH2 responses, and intestinal mast cell hyperplasia compared with wild-type mice. Anaphylaxis was abrogated in F709 mice lacking IgE or the high-affinity receptor for IgE (FcεRI). Conclusion: Augmented IL-4Rα signaling confers increased intestinal permeability and dramatically enhanced sensitivity to food allergens. Unlike anaphylaxis to injected antigens, which in rodents can be mediated by either IgE or IgG antibodies, the food-induced response in F709 mice is solely IgE dependent.

Original languageEnglish
Pages (from-to)795-805
Number of pages11
JournalJournal of Allergy and Clinical Immunology
Volume127
Issue number3
DOIs
Publication statusPublished - Mar 1 2011
Externally publishedYes

Fingerprint

Interleukin-4 Receptors
Anaphylaxis
Immunoglobulin E
Ovalbumin
Antigens
Food
Mast Cells
Antibody Formation
Permeability
Hypersensitivity
Immunoglobulin G
Genotype
IgE Receptors
Wild Animals
Cholera Toxin
Genetic Polymorphisms
Interleukin-4
Allergens
Small Intestine
Hyperplasia

Keywords

  • anaphylaxis
  • Food allergy
  • IgE
  • IL-4
  • tolerance

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling. / Mathias, Clinton B.; Hobson, Suejy A.; Garcia-Lloret, Maria; Lawson, Greg; Poddighe, Dimitri; Freyschmidt, Eva Jasmin; Xing, Wei; Gurish, Michael F.; Chatila, Talal A.; Oettgen, Hans C.

In: Journal of Allergy and Clinical Immunology, Vol. 127, No. 3, 01.03.2011, p. 795-805.

Research output: Contribution to journalArticle

Mathias, CB, Hobson, SA, Garcia-Lloret, M, Lawson, G, Poddighe, D, Freyschmidt, EJ, Xing, W, Gurish, MF, Chatila, TA & Oettgen, HC 2011, 'IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling', Journal of Allergy and Clinical Immunology, vol. 127, no. 3, pp. 795-805. https://doi.org/10.1016/j.jaci.2010.11.009
Mathias, Clinton B. ; Hobson, Suejy A. ; Garcia-Lloret, Maria ; Lawson, Greg ; Poddighe, Dimitri ; Freyschmidt, Eva Jasmin ; Xing, Wei ; Gurish, Michael F. ; Chatila, Talal A. ; Oettgen, Hans C. / IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling. In: Journal of Allergy and Clinical Immunology. 2011 ; Vol. 127, No. 3. pp. 795-805.
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AU - Hobson, Suejy A.

AU - Garcia-Lloret, Maria

AU - Lawson, Greg

AU - Poddighe, Dimitri

AU - Freyschmidt, Eva Jasmin

AU - Xing, Wei

AU - Gurish, Michael F.

AU - Chatila, Talal A.

AU - Oettgen, Hans C.

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N2 - Background: In atopic subjects food ingestion drives the production of IgE antibodies that can trigger hypersensitivity reactions. The IL-4 pathway plays a critical role in this response, and genetic polymorphisms in its components have been linked to allergy. Objective: We sought to test whether an activating mutation in the IL-4 receptor (IL-4R) α chain enhances allergic responses to a food antigen. Methods: F709 mice, in which the IL-4Rα immunoreceptor tyrosine-based inhibitory motif is inactivated, were gavage fed with ovalbumin (OVA). Reactions to OVA challenge and immune responses, including antibody production and TH2 responses, were assessed. Results: F709 mice, but not wild-type control animals, sensitized by means of gavage with OVA and either cholera toxin or staphylococcal enterotoxin B, displayed mast cell activation and systemic anaphylaxis on enteral challenge. Anaphylaxis was elicited even in F709 mice enterally sensitized with OVA alone. Bone marrow chimera experiments established that the increased sensitivity conferred by the F709 genotype was mediated mostly by hematopoietic cells but that nonhematopoietic cells also contributed. F709 mice exhibited increased intestinal permeability to macromolecules. The F709 genotype conferred increased OVA-specific IgE but not IgG1 responses, local and systemic TH2 responses, and intestinal mast cell hyperplasia compared with wild-type mice. Anaphylaxis was abrogated in F709 mice lacking IgE or the high-affinity receptor for IgE (FcεRI). Conclusion: Augmented IL-4Rα signaling confers increased intestinal permeability and dramatically enhanced sensitivity to food allergens. Unlike anaphylaxis to injected antigens, which in rodents can be mediated by either IgE or IgG antibodies, the food-induced response in F709 mice is solely IgE dependent.

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