TY - JOUR
T1 - Imaging Flow Cytometry
T2 - Coping with Heterogeneity in Biological Systems
AU - Barteneva, Natasha S.
AU - Fasler-Kan, Elizaveta
AU - Vorobjev, Ivan A.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/10
Y1 - 2012/10
N2 - Imaging flow cytometry (IFC) platforms combine features of flow cytometry and fluorescent microscopy with advances in data-processing algorithms. IFC allows multiparametric fluorescent and morphological analysis of thousands of cellular events and has the unique capability of identifying collected events by their real images. IFC allows the analysis of heterogeneous cell populations, where one of the cellular components has low expression (<0.03%) and can be described by Poisson distribution. With the help of IFC, one can address a critical question of statistical analysis of subcellular distribution of proteins in a cell. Here the authors review advantages of IFC in comparison with more traditional technologies, such as Western blotting and flow cytometry (FC), as well as new high-throughput fluorescent microscopy (HTFM), and discuss further developments of this novel analytical technique.
AB - Imaging flow cytometry (IFC) platforms combine features of flow cytometry and fluorescent microscopy with advances in data-processing algorithms. IFC allows multiparametric fluorescent and morphological analysis of thousands of cellular events and has the unique capability of identifying collected events by their real images. IFC allows the analysis of heterogeneous cell populations, where one of the cellular components has low expression (<0.03%) and can be described by Poisson distribution. With the help of IFC, one can address a critical question of statistical analysis of subcellular distribution of proteins in a cell. Here the authors review advantages of IFC in comparison with more traditional technologies, such as Western blotting and flow cytometry (FC), as well as new high-throughput fluorescent microscopy (HTFM), and discuss further developments of this novel analytical technique.
KW - Poisson distribution
KW - biological heterogeneity
KW - cellular morphology
KW - flow cytometry
KW - fluorescence
KW - high-throughput fluorescent microscopy
KW - imaging flow cytometry
KW - single cell
UR - http://www.scopus.com/inward/record.url?scp=84869824905&partnerID=8YFLogxK
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U2 - 10.1369/0022155412453052
DO - 10.1369/0022155412453052
M3 - Review article
C2 - 22740345
AN - SCOPUS:84869824905
VL - 60
SP - 723
EP - 733
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 10
ER -