Imaging Flow Cytometry: Coping with Heterogeneity in Biological Systems

Natasha S. Barteneva, Elizaveta Fasler-Kan, Ivan A. Vorobjev

Research output: Contribution to journalReview article

62 Citations (Scopus)

Abstract

Imaging flow cytometry (IFC) platforms combine features of flow cytometry and fluorescent microscopy with advances in data-processing algorithms. IFC allows multiparametric fluorescent and morphological analysis of thousands of cellular events and has the unique capability of identifying collected events by their real images. IFC allows the analysis of heterogeneous cell populations, where one of the cellular components has low expression (<0.03%) and can be described by Poisson distribution. With the help of IFC, one can address a critical question of statistical analysis of subcellular distribution of proteins in a cell. Here the authors review advantages of IFC in comparison with more traditional technologies, such as Western blotting and flow cytometry (FC), as well as new high-throughput fluorescent microscopy (HTFM), and discuss further developments of this novel analytical technique.

Original languageEnglish
Pages (from-to)723-733
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume60
Issue number10
DOIs
Publication statusPublished - Oct 1 2012

Keywords

  • Poisson distribution
  • biological heterogeneity
  • cellular morphology
  • flow cytometry
  • fluorescence
  • high-throughput fluorescent microscopy
  • imaging flow cytometry
  • single cell

ASJC Scopus subject areas

  • Anatomy
  • Histology

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