Imaging flow cytometry for phytoplankton analysis

Veronika Dashkova, Dmitry Malashenkov, Nicole Poulton, Ivan Vorobjev, Natasha S. Barteneva

Research output: Contribution to journalReview article

25 Citations (Scopus)

Abstract

This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes many of the limitations of current techniques and contributed significantly to the advancement of phytoplankton analysis in recent years. This review presents the various instrumentation relevant to the field and currently used for assessment of complex phytoplankton communities’ composition and abundance, size structure determination, biovolume estimation, detection of harmful algal bloom species, evaluation of viability and metabolic activity and other applications. Also we present our data on viability and metabolic assessment of Aphanizomenon sp. cyanobacteria using Imagestream X Mark II imaging cytometer. Herein, we highlight the immense potential of imaging flow cytometry for microalgal research, but also discuss limitations and future developments.

Original languageEnglish
Pages (from-to)188-200
Number of pages13
JournalMethods
Volume112
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • Imaging flow cytometry
  • Metabolic activity
  • Microalgae
  • Phytoplankton
  • Viability

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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