Imaging of surface cell antigens on the tumor sections of lymph nodes using fluorescence quantum dots

E. P. Raphalovskaya-Orlovskaya, L. Gorgidze, A. A. Gladkin, S. M. Tauger, I. A. Vorobjev

Research output: Contribution to journalArticlepeer-review

Abstract

The usefulness of quantum dots for the immunofluorescent detection of surface antigens on the lymphoid cells has been studied. To optimize quantum dots detection we have upgraded fluorescent microscope that allows obtaining multiple images from different quantum dots from one section. Specimens stained with quantum dots remained stable over two weeks and practically did not bleach under mercury lamp illumination during tens of minutes. Direct conjugates of primary mouse monoclonal antibodies with quantum dots demonstrated high specificity and sufficient sensitivity in the case of double staining on the frozen sections. Because of the high stability of quantum dots' fluorescence, this method allows to analyze antigen coexpression on the lymphoid tissue sections for diagnostic purposes. The spillover of fluorescent signals from quantum dots into adjacent fluorescent channels, with maxima differing by 40 nm, did not exceed 8 %, which makes the spectral compensation is practically unnecessary.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalTsitologiya
Volume54
Issue number2
Publication statusPublished - Dec 1 2012

Keywords

  • Differential diagnostics of lymphoma
  • Immunofluorescent method
  • Immunohistochemical investigations
  • Quantum dots

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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