Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production

Rosa Aragão Börner, Oksana Zaushitsyna, Dmitriy Berillo, Nazareno Scaccia, Bo Mattiasson, Harald Kirsebom

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

In this study, a new cell immobilization technique is presented. Cells of Clostridium acetobutylicum DSM 792 form a macroporous aggregate through cryogelation with concomitant crosslinking together with activated polyethyleneimine (PEI) and poly(vinyl alcohol) (PVA). The cell based cryogel presents a highly porous, elastic structure with walls consisting of densely packed crosslinked cells. The immobilization process maintained the viability of cells as new bacterial cells were observed when gel-plugs were incubated in liquid medium, glucose was consumed and solvent production was observed. Solvent production was improved 2.7-fold in immobilized cells in comparison to free cells. It was possible to reuse the gel-plugs 3-5 times in partial or completely fresh medium, reaching a maximum butanol concentration in the broth of 18.2 g/l and yield of 0.41 (g/g) in one of the cycles. The use of cells based cryogels can be a good alternative for improvement of acetone-butanol-ethanol (ABE) process as cells are immobilized in a macroporous structure with low limitations for mass transfer with potential for high yield production.

Original languageEnglish
Pages (from-to)10-18
Number of pages9
JournalProcess Biochemistry
Volume49
Issue number1
DOIs
Publication statusPublished - Jan 1 2014

Keywords

  • Butanol
  • Cell immobilization
  • Clostridium acetobutylicum
  • Cryogel

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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