Immortalized human pituitary cells express glycoprotein α-subunit and thyrotropin β (TSHβ)

J. Ham, J. Webster, J. A. Bond, B. Jasani, M. D. Lewis, P. J. Hepburn, J. S. Davies, B. M. Lewis, D. Wynford Thomas, M. F. Scanlon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A major problem in the study of human pituitary cells is their lack of proliferative capacity in vitro. To address this issue, we have infected normal human, postmortem pituitary cells in monolayer culture with a temperature-sensitive (tsA58) mutant of SV40 large T antigen. Several epithelial-like colonies were isolated; and one, designated CHP2, has been studied in detail to identify its functional characteristics. CHP2 cells have undergone more than 150 culture passages and retain an epithelial morphology. They exhibit tight temperature-dependent growth, in the presence and absence of serum, with cell division at 33 C and growth inhibition at 39 C. CHP2 cells, at both temperatures, showed diffuse immunostaining for human α- subunit and focal staining for TSHβ. Gene expression was confirmed by RT- PCR and sequencing. TRH and GnRH receptors Were not detectable, and their absence was confirmed by their lack of effects on intracellular calcium and inositol phospholipids. Cytogenetic analysis showed that the cells had a modal peak in the diploid range and a smaller peak in the tetraploid range. There was also a consistent loss of chromosome 22 and a normal chromosome 2 homologue, the latter being replaced by one of two chromosome 2 markers, M2A or M2B. In conclusion, we have immortalized human pituitary cells using SV40 tsT, from whicH we have cloned a cell line expressing a-subunit and TSHβ.

Original languageEnglish
Pages (from-to)1598-1603
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume83
Issue number5
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Thyrotropin
Chromosomes
Glycoproteins
Thyrotropin Releasing Hormone Receptors
Cells
LHRH Receptors
Chromosomes, Human, Pair 2
Viral Tumor Antigens
Phosphatidylinositols
Gene expression
Temperature
Monolayers
Calcium
Polyomavirus Transforming Antigens
Chromosomes, Human, Pair 22
Tetraploidy
Cytogenetic Analysis
Growth
Diploidy
Genetic Markers

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Ham, J., Webster, J., Bond, J. A., Jasani, B., Lewis, M. D., Hepburn, P. J., ... Scanlon, M. F. (1998). Immortalized human pituitary cells express glycoprotein α-subunit and thyrotropin β (TSHβ). Journal of Clinical Endocrinology and Metabolism, 83(5), 1598-1603. https://doi.org/10.1210/jc.83.5.1598

Immortalized human pituitary cells express glycoprotein α-subunit and thyrotropin β (TSHβ). / Ham, J.; Webster, J.; Bond, J. A.; Jasani, B.; Lewis, M. D.; Hepburn, P. J.; Davies, J. S.; Lewis, B. M.; Wynford Thomas, D.; Scanlon, M. F.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 83, No. 5, 1998, p. 1598-1603.

Research output: Contribution to journalArticle

Ham, J, Webster, J, Bond, JA, Jasani, B, Lewis, MD, Hepburn, PJ, Davies, JS, Lewis, BM, Wynford Thomas, D & Scanlon, MF 1998, 'Immortalized human pituitary cells express glycoprotein α-subunit and thyrotropin β (TSHβ)', Journal of Clinical Endocrinology and Metabolism, vol. 83, no. 5, pp. 1598-1603. https://doi.org/10.1210/jc.83.5.1598
Ham, J. ; Webster, J. ; Bond, J. A. ; Jasani, B. ; Lewis, M. D. ; Hepburn, P. J. ; Davies, J. S. ; Lewis, B. M. ; Wynford Thomas, D. ; Scanlon, M. F. / Immortalized human pituitary cells express glycoprotein α-subunit and thyrotropin β (TSHβ). In: Journal of Clinical Endocrinology and Metabolism. 1998 ; Vol. 83, No. 5. pp. 1598-1603.
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