Immunocytochemical identification of metallothionein-positive cells in rheumatoid synovium and analysis of their cell lineage

C. Winters, B. Jasani, S. Marchant, A. J. Morgan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Metallothionein is a ubiquitous low molecular weight metalloprotein with powerful protective properties against oxygen radical-mediated cytotoxicity associated with inflammatory processes. In rheumatoid arthritis, the inflammatory damage to the synovium appears to be mediated by free radicals released by the high concentration of neutrophils found in the synovial fluid of the inflamed joint. Synovial tissue obtained during routine surgery on rheumatoid and non-rheumatoid joints was subjected to an indirect immunoperoxidase protocol for the immunolocalization of metallothionein using mouse monoclonal anti-metallothionein antibody E9, reactive against the two major isoforms of mammalian metallothionein. A layer of large dendritic-like cells situated subsynovially in the rheumatoid synovium stained very positively for the metalloprotein, both cytoplasmically and in their nuclei. These cells were not found in non-rheumatoid osteoarthritic or in undamaged synovial tissue associated with traumatic joint injury. An attempt was made to investigate their lineage using a series of antibody markers against epithelial cells, endothelial cells, smooth muscle, mesothelial cells, fibroblasts, neutrophils, dermal dendrocytes, macrophages, low and high molecular weight cytokeratin as well as a cell proliferation marker. From our results, it is suggested that these metallothionein-positive cells are probably myofibroblasts similar to the highly motile cells present in granulation tissue. They may originate from perivascular areas of synovium and their movement into the inflamed synovium may reflect the cytoprotective role of metallothionein acting as a free radical scavenger against oxidative damage.

Original languageEnglish
Pages (from-to)301-307
Number of pages7
JournalThe Histochemical Journal
Volume29
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Synovial Membrane
Metallothionein
Cell Lineage
Metalloproteins
Joints
Neutrophils
Molecular Weight
Free Radical Scavengers
Myofibroblasts
Granulation Tissue
Synovial Fluid
Keratins
Dendritic Cells
Free Radicals
Smooth Muscle Myocytes
Anti-Idiotypic Antibodies
Reactive Oxygen Species
Rheumatoid Arthritis
Protein Isoforms
Endothelial Cells

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Immunocytochemical identification of metallothionein-positive cells in rheumatoid synovium and analysis of their cell lineage. / Winters, C.; Jasani, B.; Marchant, S.; Morgan, A. J.

In: The Histochemical Journal, Vol. 29, No. 4, 1997, p. 301-307.

Research output: Contribution to journalArticle

Winters, C. ; Jasani, B. ; Marchant, S. ; Morgan, A. J. / Immunocytochemical identification of metallothionein-positive cells in rheumatoid synovium and analysis of their cell lineage. In: The Histochemical Journal. 1997 ; Vol. 29, No. 4. pp. 301-307.
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