Abstract
The possible role of changes in TGF-β expression in the multistage development of thyroid cancer was assessed. The presence of TGF-β1 in thyroid epithelial cells was analyzed in sections of normal and tumor tissue using an immunoperoxidase technique employing an antibody directed against the amino-terminal 30 amino acids of mature TGF-β1. Specific immunostaining was clearly detected in epithelial cells in 58% of malignant thyroid tumours (including follicular, papillary, and anaplastic variants). However, no positive cells were seen in any of 7 benign tumors nor in any normal thyroid epithelium. Within the cancer group as a whole, there was no significant correlation with pathological grade or clinical stage of tumor but in one subgroup-follicular carcinomas-a significant association was noted between TGF-β immunostaining and the presence of a specific mutation of the H-ras oncogene (codon 61, glnarg). We conclude that a major alteration in expression of TGF-β occurs specifically in the malignant stage of tumor development in thyroid follicular epithelium and speculate on its possible role in this process.
| Original language | English |
|---|---|
| Pages (from-to) | 149-155 |
| Number of pages | 7 |
| Journal | Growth Factors |
| Volume | 2 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1990 |
| Externally published | Yes |
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Keywords
- Cancer
- Epithelial cells
- TGF-β
- Thyroid
ASJC Scopus subject areas
- Clinical Biochemistry
- Endocrinology
- Cell Biology
Cite this
Immunocytochemically detectable TGF-β associated with malignancy in thyroid epithelial neoplasia. / Jasani, B.; Wyllie, F. S.; Wright, P. A.; Lemoine, N. R.; Williams, E. D.; Wynford-Thomas, D.
In: Growth Factors, Vol. 2, No. 2, 1990, p. 149-155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunocytochemically detectable TGF-β associated with malignancy in thyroid epithelial neoplasia
AU - Jasani, B.
AU - Wyllie, F. S.
AU - Wright, P. A.
AU - Lemoine, N. R.
AU - Williams, E. D.
AU - Wynford-Thomas, D.
PY - 1990
Y1 - 1990
N2 - The possible role of changes in TGF-β expression in the multistage development of thyroid cancer was assessed. The presence of TGF-β1 in thyroid epithelial cells was analyzed in sections of normal and tumor tissue using an immunoperoxidase technique employing an antibody directed against the amino-terminal 30 amino acids of mature TGF-β1. Specific immunostaining was clearly detected in epithelial cells in 58% of malignant thyroid tumours (including follicular, papillary, and anaplastic variants). However, no positive cells were seen in any of 7 benign tumors nor in any normal thyroid epithelium. Within the cancer group as a whole, there was no significant correlation with pathological grade or clinical stage of tumor but in one subgroup-follicular carcinomas-a significant association was noted between TGF-β immunostaining and the presence of a specific mutation of the H-ras oncogene (codon 61, glnarg). We conclude that a major alteration in expression of TGF-β occurs specifically in the malignant stage of tumor development in thyroid follicular epithelium and speculate on its possible role in this process.
AB - The possible role of changes in TGF-β expression in the multistage development of thyroid cancer was assessed. The presence of TGF-β1 in thyroid epithelial cells was analyzed in sections of normal and tumor tissue using an immunoperoxidase technique employing an antibody directed against the amino-terminal 30 amino acids of mature TGF-β1. Specific immunostaining was clearly detected in epithelial cells in 58% of malignant thyroid tumours (including follicular, papillary, and anaplastic variants). However, no positive cells were seen in any of 7 benign tumors nor in any normal thyroid epithelium. Within the cancer group as a whole, there was no significant correlation with pathological grade or clinical stage of tumor but in one subgroup-follicular carcinomas-a significant association was noted between TGF-β immunostaining and the presence of a specific mutation of the H-ras oncogene (codon 61, glnarg). We conclude that a major alteration in expression of TGF-β occurs specifically in the malignant stage of tumor development in thyroid follicular epithelium and speculate on its possible role in this process.
KW - Cancer
KW - Epithelial cells
KW - TGF-β
KW - Thyroid
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U2 - 10.3109/08977199009071501
DO - 10.3109/08977199009071501
M3 - Article
VL - 2
SP - 149
EP - 155
JO - Growth Factors
JF - Growth Factors
SN - 0897-7194
IS - 2
ER -