TY - JOUR
T1 - Immunoelectronmicroscopy
T2 - Immunogold and immunoperoxidase compared using a new post-embedding system
AU - Newman, G. R.
AU - Jasani, B.
PY - 1984/12/1
Y1 - 1984/12/1
N2 - Immunogold and immunoperoxidase methods have been compared on ultra-thin sections of the same tissue prepared by a new system of fixation and embedding. Surgically removed human pituitary was fixed in buffered glutaraldehyde/picric acid and, without osmium post-fixation, embedded in a new acrylic plastic, LR White, in order to preserve both antigenicity and ultrastructure. Ultra-thin sections, without any pre-treatment such as etching or trypsin digestion, were than exposed to dinitrophenyl (DNA)-labelled anti-'Synacthen', an antibody to human ACTH. Some of these sections were then stained with colloidal gold using the indirect immunogold staining method and others using a DNP-hapten bridge antibody immunoperoxidase technique. The merits and demerits of the two immunotechniques are discussed.
AB - Immunogold and immunoperoxidase methods have been compared on ultra-thin sections of the same tissue prepared by a new system of fixation and embedding. Surgically removed human pituitary was fixed in buffered glutaraldehyde/picric acid and, without osmium post-fixation, embedded in a new acrylic plastic, LR White, in order to preserve both antigenicity and ultrastructure. Ultra-thin sections, without any pre-treatment such as etching or trypsin digestion, were than exposed to dinitrophenyl (DNA)-labelled anti-'Synacthen', an antibody to human ACTH. Some of these sections were then stained with colloidal gold using the indirect immunogold staining method and others using a DNP-hapten bridge antibody immunoperoxidase technique. The merits and demerits of the two immunotechniques are discussed.
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M3 - Article
C2 - 6384716
AN - SCOPUS:0021685447
VL - 41
SP - 238
EP - 245
JO - British Journal of Biomedical Science
JF - British Journal of Biomedical Science
SN - 0967-4845
IS - 3
ER -