Abstract
By using a double-affmity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.
| Original language | English |
|---|---|
| Pages (from-to) | 265-270 |
| Number of pages | 6 |
| Journal | Cell and Tissue Research |
| Volume | 244 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - May 1986 |
| Externally published | Yes |
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Keywords
- Calcium-activated proteinase
- Calpain I
- EDL muscle
- Immunogold technique
- Wistar rats
ASJC Scopus subject areas
- Anatomy
- Clinical Biochemistry
- Cell Biology
Cite this
Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats. / Yoshimura, Nagahisa; Murachi, Takashi; Heath, Robert; Kay, John; Jasani, Bharat; Newman, Geoffrey R.
In: Cell and Tissue Research, Vol. 244, No. 2, 05.1986, p. 265-270.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats
AU - Yoshimura, Nagahisa
AU - Murachi, Takashi
AU - Heath, Robert
AU - Kay, John
AU - Jasani, Bharat
AU - Newman, Geoffrey R.
PY - 1986/5
Y1 - 1986/5
N2 - By using a double-affmity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.
AB - By using a double-affmity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.
KW - Calcium-activated proteinase
KW - Calpain I
KW - EDL muscle
KW - Immunogold technique
KW - Wistar rats
UR - http://www.scopus.com/inward/record.url?scp=0022509727&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022509727&partnerID=8YFLogxK
U2 - 10.1007/BF00219201
DO - 10.1007/BF00219201
M3 - Article
C2 - 3013409
AN - SCOPUS:0022509727
VL - 244
SP - 265
EP - 270
JO - Cell and Tissue Research
JF - Cell and Tissue Research
SN - 0302-766X
IS - 2
ER -