Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats

Nagahisa Yoshimura; Takashi Murachi; Robert Heath; John Kay; Bharat Jasani; Geoffrey R. Newman

doi:10.1007/BF00219201

Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats

Nagahisa Yoshimura, Takashi Murachi, Robert Heath, John Kay, Bharat Jasani, Geoffrey R. Newman

School of Medicine

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

By using a double-affmity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca²⁺) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.

Original language	English
Pages (from-to)	265-270
Number of pages	6
Journal	Cell and Tissue Research
Volume	244
Issue number	2
DOIs	https://doi.org/10.1007/BF00219201
Publication status	Published - May 1986

Keywords

Calcium-activated proteinase
Calpain I
EDL muscle
Immunogold technique
Wistar rats

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology
Cell Biology

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title = "Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats",

abstract = "By using a double-affmity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.",

keywords = "Calcium-activated proteinase, Calpain I, EDL muscle, Immunogold technique, Wistar rats",

author = "Nagahisa Yoshimura and Takashi Murachi and Robert Heath and John Kay and Bharat Jasani and Newman, {Geoffrey R.}",

year = "1986",

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language = "English",

volume = "244",

pages = "265--270",

journal = "Cell and Tissue Research",

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T1 - Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats

AU - Yoshimura, Nagahisa

AU - Murachi, Takashi

AU - Heath, Robert

AU - Kay, John

AU - Jasani, Bharat

AU - Newman, Geoffrey R.

PY - 1986/5

Y1 - 1986/5

N2 - By using a double-affmity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.

AB - By using a double-affmity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.

KW - Calcium-activated proteinase

KW - Calpain I

KW - EDL muscle

KW - Immunogold technique

KW - Wistar rats

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JO - Cell and Tissue Research

JF - Cell and Tissue Research

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