Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats

Nagahisa Yoshimura, Takashi Murachi, Robert Heath, John Kay, Bharat Jasani, Geoffrey R. Newman

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

By using a double-affmity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalCell and Tissue Research
Volume244
Issue number2
DOIs
Publication statusPublished - May 1 1986

Keywords

  • Calcium-activated proteinase
  • Calpain I
  • EDL muscle
  • Immunogold technique
  • Wistar rats

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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    Yoshimura, N., Murachi, T., Heath, R., Kay, J., Jasani, B., & Newman, G. R. (1986). Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats. Cell and Tissue Research, 244(2), 265-270. https://doi.org/10.1007/BF00219201