Abstract
A method is presented which allows correlative serial section analysis by light and electron microscopy of cell surface antigens in monolayer cultures. Sites of antigenicity are shown by deposition of diaminobenzidine after pre-embedding, immunoperoxidase immunocytochemistry. Osmication is replaced by the use of gold chloride which specifically enhances the electron density of diaminobenzidine. In addition gold chloride bound to diaminobenzidine survives embedding and provides the basis for a post-embedding photochemical amplification method. Immunostained cells are embedded in LR White by a rapid technique which preserves their structure and leaves them available for subsequent post-embedding immunocytochemistry. The method is illustrated by the demonstration of epidermal growth factor (EGF) receptors on the EGF receptor-rich human carcinoma cell line A431 using a well characterized monoclonal antibody raised against EGF receptor.
| Original language | English |
|---|---|
| Pages (from-to) | 387-396 |
| Number of pages | 10 |
| Journal | The Histochemical Journal |
| Volume | 18 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
Immunohistochemical localization of cell surface receptors using a novel method permitting simple, rapid and reliable LM/EM correlation. / Wynford-Thomas, David; Jasani, Bharat; Newman, Geoffrey R.
In: The Histochemical Journal, Vol. 18, No. 7, 07.1986, p. 387-396.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunohistochemical localization of cell surface receptors using a novel method permitting simple, rapid and reliable LM/EM correlation
AU - Wynford-Thomas, David
AU - Jasani, Bharat
AU - Newman, Geoffrey R.
PY - 1986/7
Y1 - 1986/7
N2 - A method is presented which allows correlative serial section analysis by light and electron microscopy of cell surface antigens in monolayer cultures. Sites of antigenicity are shown by deposition of diaminobenzidine after pre-embedding, immunoperoxidase immunocytochemistry. Osmication is replaced by the use of gold chloride which specifically enhances the electron density of diaminobenzidine. In addition gold chloride bound to diaminobenzidine survives embedding and provides the basis for a post-embedding photochemical amplification method. Immunostained cells are embedded in LR White by a rapid technique which preserves their structure and leaves them available for subsequent post-embedding immunocytochemistry. The method is illustrated by the demonstration of epidermal growth factor (EGF) receptors on the EGF receptor-rich human carcinoma cell line A431 using a well characterized monoclonal antibody raised against EGF receptor.
AB - A method is presented which allows correlative serial section analysis by light and electron microscopy of cell surface antigens in monolayer cultures. Sites of antigenicity are shown by deposition of diaminobenzidine after pre-embedding, immunoperoxidase immunocytochemistry. Osmication is replaced by the use of gold chloride which specifically enhances the electron density of diaminobenzidine. In addition gold chloride bound to diaminobenzidine survives embedding and provides the basis for a post-embedding photochemical amplification method. Immunostained cells are embedded in LR White by a rapid technique which preserves their structure and leaves them available for subsequent post-embedding immunocytochemistry. The method is illustrated by the demonstration of epidermal growth factor (EGF) receptors on the EGF receptor-rich human carcinoma cell line A431 using a well characterized monoclonal antibody raised against EGF receptor.
UR - http://www.scopus.com/inward/record.url?scp=0022748619&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022748619&partnerID=8YFLogxK
U2 - 10.1007/BF01675220
DO - 10.1007/BF01675220
M3 - Article
C2 - 3531102
AN - SCOPUS:0022748619
VL - 18
SP - 387
EP - 396
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
SN - 1567-2379
IS - 7
ER -