Immunohistochemical retrieval of the principal HIV antigens p24, gp41, and gp 120 in formalin fixed tissue

An investigation using HIV infected lymphoblasts and postmortem brain tissue from AIDS cases

H. L. Morrison, J. W. Neal, A. B. Parkes, B. Jasani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This paper describes the use of an autoclaving procedure followed by immunocytochemistry to enhance the detection of the human immunodeficiency virus (HIV) antigens p24, gp41, and gp120. This procedure greatly improved the detection rate of the p24 and gp41 HIV surface antigens in formalin fixed, paraffin wax embedded, HIV positive central nervous system (CNS) tissue while restricting staining to areas of the CNS showing evidence of neuropathology. However, the technique did not improve retrieval of the gp120 antigen in either HIV positive, formalin fixed CNS tissue or HIV infected T lymphoblasts. The inclusion of the high temperature autoclave step was validated using both HIV infected lymphoblasts and preadsorption of the specific antibodies with the appropriate recombinant HIV proteins. Using the methodology described here, formalin fixed CNS tissue from potential or known HIV positive cases can be processed reliably and safely. To ensure the reliability of this technique, it is recommended that an assessment of both the p24 and gp41 antigens is undertaken.

Original languageEnglish
Pages (from-to)227-231
Number of pages5
JournalJournal of Clinical Pathology
Volume51
Issue number4
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Formaldehyde
Acquired Immunodeficiency Syndrome
HIV
Antigens
Nerve Tissue
Brain
Central Nervous System
Human Immunodeficiency Virus Proteins
Waxes
Surface Antigens
Recombinant Proteins
Paraffin
Immunohistochemistry
Staining and Labeling
Temperature
Antibodies

Keywords

  • AIDS dementia
  • High temperature antigen retrieval
  • Human immunodeficiency virus

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

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title = "Immunohistochemical retrieval of the principal HIV antigens p24, gp41, and gp 120 in formalin fixed tissue: An investigation using HIV infected lymphoblasts and postmortem brain tissue from AIDS cases",
abstract = "This paper describes the use of an autoclaving procedure followed by immunocytochemistry to enhance the detection of the human immunodeficiency virus (HIV) antigens p24, gp41, and gp120. This procedure greatly improved the detection rate of the p24 and gp41 HIV surface antigens in formalin fixed, paraffin wax embedded, HIV positive central nervous system (CNS) tissue while restricting staining to areas of the CNS showing evidence of neuropathology. However, the technique did not improve retrieval of the gp120 antigen in either HIV positive, formalin fixed CNS tissue or HIV infected T lymphoblasts. The inclusion of the high temperature autoclave step was validated using both HIV infected lymphoblasts and preadsorption of the specific antibodies with the appropriate recombinant HIV proteins. Using the methodology described here, formalin fixed CNS tissue from potential or known HIV positive cases can be processed reliably and safely. To ensure the reliability of this technique, it is recommended that an assessment of both the p24 and gp41 antigens is undertaken.",
keywords = "AIDS dementia, High temperature antigen retrieval, Human immunodeficiency virus",
author = "Morrison, {H. L.} and Neal, {J. W.} and Parkes, {A. B.} and B. Jasani",
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AU - Neal, J. W.

AU - Parkes, A. B.

AU - Jasani, B.

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N2 - This paper describes the use of an autoclaving procedure followed by immunocytochemistry to enhance the detection of the human immunodeficiency virus (HIV) antigens p24, gp41, and gp120. This procedure greatly improved the detection rate of the p24 and gp41 HIV surface antigens in formalin fixed, paraffin wax embedded, HIV positive central nervous system (CNS) tissue while restricting staining to areas of the CNS showing evidence of neuropathology. However, the technique did not improve retrieval of the gp120 antigen in either HIV positive, formalin fixed CNS tissue or HIV infected T lymphoblasts. The inclusion of the high temperature autoclave step was validated using both HIV infected lymphoblasts and preadsorption of the specific antibodies with the appropriate recombinant HIV proteins. Using the methodology described here, formalin fixed CNS tissue from potential or known HIV positive cases can be processed reliably and safely. To ensure the reliability of this technique, it is recommended that an assessment of both the p24 and gp41 antigens is undertaken.

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