Immunomodulating activity of chemotactic peptide conjugated with liposomal antigen

K. P. Kashkin, L. N. Dmitrieva, V. N. Medvedkin, L. V. Shepshelevich, L. V. Klimenko, N. S. Barteneva, D. V. Ioffe

Research output: Contribution to journalArticle

Abstract

The capacity for changing immunogenicity of bovine serum albumin (BSA) and for inducing primary and secondary immune response in mice against BSA was investigated in the macrophage-activating chemotactic peptide N-f-Met-Leu-Phe-Gly conjugated on the basis of liposomas with BSA. To elucidate the mechanism of immunomodulating activity of chemotactic peptide (CP) with an antigen, the capacity of the synthetic immunogen for inducing macrophage-dependent and independent lymphocyte proliferation was evaluated. It has been shown that coimmobilization of BSA and CP based on lyposomas permits obtaining preparations that being injected subcutaneously induce in CBF1 mice humoral immune response similar to that observed when BSA with Freund's adjuvant is administered. CP has a macrophage-mediated mitogenic action upon mouse splenocytes. Immunomodulating activity of CP is manifested only in cases when the immunomodulator and antigen are coimmobilized on the surface of liposomas (are involved into the lipid bi-layer of liposomas). It is suggested that the immunostimulating effect observed is caused by immunomodulator capacity for involving the maximum number of clones of the antigen-reactive cells into the immune response to CP immobilized with the antigen.

Original languageEnglish
JournalImmunologiya
Issue number6
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Bovine Serum Albumin
Antigens
Peptides
Macrophages
Immunologic Factors
Synthetic Vaccines
Freund's Adjuvant
Humoral Immunity
Clone Cells
Lymphocytes
Lipids

ASJC Scopus subject areas

  • Immunology

Cite this

Kashkin, K. P., Dmitrieva, L. N., Medvedkin, V. N., Shepshelevich, L. V., Klimenko, L. V., Barteneva, N. S., & Ioffe, D. V. (1987). Immunomodulating activity of chemotactic peptide conjugated with liposomal antigen. Immunologiya, (6).

Immunomodulating activity of chemotactic peptide conjugated with liposomal antigen. / Kashkin, K. P.; Dmitrieva, L. N.; Medvedkin, V. N.; Shepshelevich, L. V.; Klimenko, L. V.; Barteneva, N. S.; Ioffe, D. V.

In: Immunologiya, No. 6, 1987.

Research output: Contribution to journalArticle

Kashkin, KP, Dmitrieva, LN, Medvedkin, VN, Shepshelevich, LV, Klimenko, LV, Barteneva, NS & Ioffe, DV 1987, 'Immunomodulating activity of chemotactic peptide conjugated with liposomal antigen', Immunologiya, no. 6.
Kashkin KP, Dmitrieva LN, Medvedkin VN, Shepshelevich LV, Klimenko LV, Barteneva NS et al. Immunomodulating activity of chemotactic peptide conjugated with liposomal antigen. Immunologiya. 1987;(6).
Kashkin, K. P. ; Dmitrieva, L. N. ; Medvedkin, V. N. ; Shepshelevich, L. V. ; Klimenko, L. V. ; Barteneva, N. S. ; Ioffe, D. V. / Immunomodulating activity of chemotactic peptide conjugated with liposomal antigen. In: Immunologiya. 1987 ; No. 6.
@article{7a8b6aed51e9475da77faa2c9e2a616f,
title = "Immunomodulating activity of chemotactic peptide conjugated with liposomal antigen",
abstract = "The capacity for changing immunogenicity of bovine serum albumin (BSA) and for inducing primary and secondary immune response in mice against BSA was investigated in the macrophage-activating chemotactic peptide N-f-Met-Leu-Phe-Gly conjugated on the basis of liposomas with BSA. To elucidate the mechanism of immunomodulating activity of chemotactic peptide (CP) with an antigen, the capacity of the synthetic immunogen for inducing macrophage-dependent and independent lymphocyte proliferation was evaluated. It has been shown that coimmobilization of BSA and CP based on lyposomas permits obtaining preparations that being injected subcutaneously induce in CBF1 mice humoral immune response similar to that observed when BSA with Freund's adjuvant is administered. CP has a macrophage-mediated mitogenic action upon mouse splenocytes. Immunomodulating activity of CP is manifested only in cases when the immunomodulator and antigen are coimmobilized on the surface of liposomas (are involved into the lipid bi-layer of liposomas). It is suggested that the immunostimulating effect observed is caused by immunomodulator capacity for involving the maximum number of clones of the antigen-reactive cells into the immune response to CP immobilized with the antigen.",
author = "Kashkin, {K. P.} and Dmitrieva, {L. N.} and Medvedkin, {V. N.} and Shepshelevich, {L. V.} and Klimenko, {L. V.} and Barteneva, {N. S.} and Ioffe, {D. V.}",
year = "1987",
language = "English",
journal = "Immunologiya",
issn = "0206-4952",
publisher = "Meditsina Publishers",
number = "6",

}

TY - JOUR

T1 - Immunomodulating activity of chemotactic peptide conjugated with liposomal antigen

AU - Kashkin, K. P.

AU - Dmitrieva, L. N.

AU - Medvedkin, V. N.

AU - Shepshelevich, L. V.

AU - Klimenko, L. V.

AU - Barteneva, N. S.

AU - Ioffe, D. V.

PY - 1987

Y1 - 1987

N2 - The capacity for changing immunogenicity of bovine serum albumin (BSA) and for inducing primary and secondary immune response in mice against BSA was investigated in the macrophage-activating chemotactic peptide N-f-Met-Leu-Phe-Gly conjugated on the basis of liposomas with BSA. To elucidate the mechanism of immunomodulating activity of chemotactic peptide (CP) with an antigen, the capacity of the synthetic immunogen for inducing macrophage-dependent and independent lymphocyte proliferation was evaluated. It has been shown that coimmobilization of BSA and CP based on lyposomas permits obtaining preparations that being injected subcutaneously induce in CBF1 mice humoral immune response similar to that observed when BSA with Freund's adjuvant is administered. CP has a macrophage-mediated mitogenic action upon mouse splenocytes. Immunomodulating activity of CP is manifested only in cases when the immunomodulator and antigen are coimmobilized on the surface of liposomas (are involved into the lipid bi-layer of liposomas). It is suggested that the immunostimulating effect observed is caused by immunomodulator capacity for involving the maximum number of clones of the antigen-reactive cells into the immune response to CP immobilized with the antigen.

AB - The capacity for changing immunogenicity of bovine serum albumin (BSA) and for inducing primary and secondary immune response in mice against BSA was investigated in the macrophage-activating chemotactic peptide N-f-Met-Leu-Phe-Gly conjugated on the basis of liposomas with BSA. To elucidate the mechanism of immunomodulating activity of chemotactic peptide (CP) with an antigen, the capacity of the synthetic immunogen for inducing macrophage-dependent and independent lymphocyte proliferation was evaluated. It has been shown that coimmobilization of BSA and CP based on lyposomas permits obtaining preparations that being injected subcutaneously induce in CBF1 mice humoral immune response similar to that observed when BSA with Freund's adjuvant is administered. CP has a macrophage-mediated mitogenic action upon mouse splenocytes. Immunomodulating activity of CP is manifested only in cases when the immunomodulator and antigen are coimmobilized on the surface of liposomas (are involved into the lipid bi-layer of liposomas). It is suggested that the immunostimulating effect observed is caused by immunomodulator capacity for involving the maximum number of clones of the antigen-reactive cells into the immune response to CP immobilized with the antigen.

UR - http://www.scopus.com/inward/record.url?scp=0023580125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023580125&partnerID=8YFLogxK

M3 - Article

JO - Immunologiya

JF - Immunologiya

SN - 0206-4952

IS - 6

ER -