Immunophenotyping of blood and lymphoid malignancies. Part I. Mature cell lymphomas and lymphosarcomas

I. A. Vorobyev, O. A. Khudoleeva, T. D. Roschupkina, E. M. Gretsov

Research output: Contribution to journalArticlepeer-review

Abstract

The method of flow fluorimetry is effective for diagnosis of lymphatic tumors and blood tumors. High sensitivity and efficiency of modern flow fluori- meters and ability to analyse coexpression of 2-3 markers make this method more sensitive and informative compared to fluorescent microscopy. Diagnostic studies on a flow fluorimeter use both peripheral blood and bone marrow, spleen, lymph nodes and some other samples. Classification of lymphatic tissue tumors by results of immunophenotyping isconducted primarily in the scope of WHO classification (2001). Basing on the data of examination of various patients we constructed a panel of monoclonal antibodies for immunophenotyping of chronic lymphatic tumors and lymphosarcomas. The panel employs antibodies conjugated with FITS, PE and PE-Cy-5. Methods of analysis of some cell and antigen populations and difficulties arising in analysis of some aggressive tumors are discussed.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalGematologiya i Transfusiologiya
Volume50
Issue number1
Publication statusPublished - Dec 1 2005

Keywords

  • Antibodies panel
  • Diagnosis
  • Flow fluorimetry
  • Lymphomas
  • Lymphomas classification
  • Lymphosarcomas
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Hematology

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