Improved reliability of lymphoma diagnostics via PCR-based clonality testing: - Report of the BIOMED-2 Concerted Action BHM4-CT98-3936

J. H.J.M. van Krieken, A. W. Langerak, E. A. Macintyre, M. Kneba, E. Hodges, R. Garcia Sanz, G. J. Morgan, A. Parreira, T. J. Molina, J. Cabeçadas, P. Gaulard, B. Jasani, J. F. Garcia, M. Ott, M. L. Hannsmann, F. Berger, M. Hummel, F. Davi, M. Brüggemann, F. L. LavenderE. Schuuring, P. A.S. Evans, H. White, G. Salles, P. J.T.A. Groenen, P. Gameiro, Ch Pott, J. J.M. van Dongen

Research output: Contribution to journalArticlepeer-review

219 Citations (Scopus)

Abstract

The diagnosis of malignant lymphoma is a recognized difficult area in histopathology. Therefore, detection of clonality in a suspected lymphoproliferation is a valuable diagnostic criterion. We have developed primer sets for the detection of rearrangements in the B- and T-cell receptor genes as reliable tools for clonality assessment in lymphoproliferations suspected for lymphoma. In this issue of Leukemia, the participants of the BIOMED-2 Concerted Action CT98-3936 report on the validation of the newly developed clonality assays in various disease entities. Clonality was detected in 99% of all B-cell malignancies and in 94% of all T-cell malignancies, whereas the great majority of reactive lesions showed polyclonality. The combined BIOMED-2 results are summarized in a guideline, which can now be implemented in routine lymphoma diagnostics. The use of this standardized approach in patients with a suspect lymphoproliferation will result in improved diagnosis of malignant lymphoma.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalLeukemia
Volume21
Issue number2
DOIs
Publication statusPublished - Feb 2007

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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