Improvement of neurological function in rats with ischemic stroke by adipose-derived pericytes

Vyacheslav Ogay, Venera Kumasheva, Yelena Li, Sholpan Mukhlis, Aliya Sekenova, Farkhad Olzhayev, Andrey Tsoy, Baurzhan Umbayev, Sholpan Askarova, Azat Shpekov, Assylbek Kaliyev, Berik Zhetpisbayev, Yerbol Makhambetov, Serik Akshulakov, Arman Saparov, Yerlan Ramankulov

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were iso-lated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neuro-genesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline– treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalCell Transplantation
Publication statusPublished - 2020


  • Angiogenesis
  • Ischemic stroke
  • Neurogenesis
  • Neurological recovery
  • Pericytes

ASJC Scopus subject areas

  • General Medicine


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