Improvement of neurological function in rats with ischemic stroke by adipose-derived pericytes

Vyacheslav Ogay; Venera Kumasheva; Yelena Li; Sholpan Mukhlis; Aliya Sekenova; Farkhad Olzhayev; Andrey Tsoy; Baurzhan Umbayev; Sholpan Askarova; Azat Shpekov; Assylbek Kaliyev; Berik Zhetpisbayev; Yerbol Makhambetov; Serik Akshulakov; Arman Saparov; Yerlan Ramankulov

doi:10.1177/0963689720956956

Improvement of neurological function in rats with ischemic stroke by adipose-derived pericytes

Vyacheslav Ogay
, Venera Kumasheva
, Yelena Li
, Sholpan Mukhlis
, Aliya Sekenova
, Farkhad Olzhayev
, Andrey Tsoy
, Baurzhan Umbayev
, Sholpan Askarova
, Azat Shpekov
, Assylbek Kaliyev
, Berik Zhetpisbayev
, Yerbol Makhambetov
, Serik Akshulakov
, Arman Saparov
, Yerlan Ramankulov

National Center for Biotechnology
Stem Cell Laboratory
The Medical Centre Hospital of the President’s Affairs Administration of the Republic of Kazakhstan
National Neurosurgery Center

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were iso-lated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neuro-genesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline– treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients.

Original language	English
Pages (from-to)	1-12
Number of pages	12
Journal	Cell Transplantation
Volume	29
DOIs	https://doi.org/10.1177/0963689720956956
Publication status	Published - 2020

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was financially supported by Ministry of Education and Science of the Republic of Kazakhstan (Grants AP05134447 and 4131/ GF4).

Keywords

Angiogenesis
Ischemic stroke
Neurogenesis
Neurological recovery
Pericytes

ASJC Scopus subject areas

General Medicine

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10.1177/0963689720956956

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Ogay, V., Kumasheva, V., Li, Y., Mukhlis, S., Sekenova, A., Olzhayev, F., Tsoy, A., Umbayev, B., Askarova, S., Shpekov, A., Kaliyev, A., Zhetpisbayev, B., Makhambetov, Y., Akshulakov, S., Saparov, A., & Ramankulov, Y. (2020). Improvement of neurological function in rats with ischemic stroke by adipose-derived pericytes. Cell Transplantation, 29, 1-12. https://doi.org/10.1177/0963689720956956

Ogay, V, Kumasheva, V, Li, Y, Mukhlis, S, Sekenova, A, Olzhayev, F , Tsoy, A , Umbayev, B , Askarova, S, Shpekov, A, Kaliyev, A, Zhetpisbayev, B, Makhambetov, Y, Akshulakov, S, Saparov, A & Ramankulov, Y 2020, 'Improvement of neurological function in rats with ischemic stroke by adipose-derived pericytes', Cell Transplantation, vol. 29, pp. 1-12. https://doi.org/10.1177/0963689720956956

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title = "Improvement of neurological function in rats with ischemic stroke by adipose-derived pericytes",

abstract = "Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were iso-lated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neuro-genesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline{\textendash} treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients.",

keywords = "Angiogenesis, Ischemic stroke, Neurogenesis, Neurological recovery, Pericytes",

author = "Vyacheslav Ogay and Venera Kumasheva and Yelena Li and Sholpan Mukhlis and Aliya Sekenova and Farkhad Olzhayev and Andrey Tsoy and Baurzhan Umbayev and Sholpan Askarova and Azat Shpekov and Assylbek Kaliyev and Berik Zhetpisbayev and Yerbol Makhambetov and Serik Akshulakov and Arman Saparov and Yerlan Ramankulov",

note = "Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was financially supported by Ministry of Education and Science of the Republic of Kazakhstan (Grants AP05134447 and 4131/ GF4). Publisher Copyright: {\textcopyright} The Author(s) 2020.",

year = "2020",

doi = "10.1177/0963689720956956",

language = "English",

volume = "29",

pages = "1--12",

journal = "Cell Transplantation",

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T1 - Improvement of neurological function in rats with ischemic stroke by adipose-derived pericytes

AU - Ogay, Vyacheslav

AU - Kumasheva, Venera

AU - Li, Yelena

AU - Mukhlis, Sholpan

AU - Sekenova, Aliya

AU - Olzhayev, Farkhad

AU - Tsoy, Andrey

AU - Umbayev, Baurzhan

AU - Askarova, Sholpan

AU - Shpekov, Azat

AU - Kaliyev, Assylbek

AU - Zhetpisbayev, Berik

AU - Makhambetov, Yerbol

AU - Akshulakov, Serik

AU - Saparov, Arman

AU - Ramankulov, Yerlan

N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was financially supported by Ministry of Education and Science of the Republic of Kazakhstan (Grants AP05134447 and 4131/ GF4). Publisher Copyright: © The Author(s) 2020.

PY - 2020

Y1 - 2020

N2 - Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were iso-lated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neuro-genesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline– treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients.

AB - Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were iso-lated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neuro-genesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline– treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients.

KW - Angiogenesis

KW - Ischemic stroke

KW - Neurogenesis

KW - Neurological recovery

KW - Pericytes

UR - https://www.scopus.com/pages/publications/85090318704

UR - https://www.scopus.com/pages/publications/85090318704#tab=citedBy

U2 - 10.1177/0963689720956956

DO - 10.1177/0963689720956956

M3 - Article

C2 - 32885682

AN - SCOPUS:85090318704

SN - 0963-6897

VL - 29

SP - 1

EP - 12

JO - Cell Transplantation

JF - Cell Transplantation

ER -

Improvement of neurological function in rats with ischemic stroke by adipose-derived pericytes

Abstract

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Keywords

ASJC Scopus subject areas

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